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Name the media used in the Kirby Bauer test. a. Mueller Hinton b. Mannitol salt c. Ashby plus d. Pseudocel a. Mueller Hinton The Kirby-Bauer technique: a. measures the susceptibility of a test microorganism to a wide range of antibiotics. b. standardizes medium variables. c. involves measuring the zone of inhibition about an antibiotic impregnated disc. d. All of the above. d. All of the above. Antibiotics display selective toxicity --this means that they a. inhibit some bacteria but not others. b. effect the functions of bacterial cells without effecting host cell functions. c. they effect viral activity and not bacterial activity. d. can sometimes act as disinfectants and sometimes as antiseptics depending upon their concentration. b. effect the functions of bacterial cells without effecting host cell functions. All E.coli are resistant to penicillin while Staphylococcus aureus is susceptible. This indicates that a. E.coli has inate resistance to penicillin. b. E.coli is a mutant that is antibiotic resistant. a. E.coli has inate resistance to penicillin. E.coli is inately resistant to penicillin. The cells produce an enzyme, Beta lactamase that destroys the antibiotic. Name the technique that allows you determine the susceptibility of an organism to antibiotics. a. Presumptive test b. Contact time test c. Kirby Bauer Test d. Growth inhibition assay c. Kirby Bauer Test This is correct. This is a standardized test that uses Mueller Hinton media and exact growth conditions. Antibiotics are molecules that are produced by _________ which kill or inhibit the growth of bacteria a. bacteria and fungi. b. animal cells c. viruses and viroids d. antibodies a. bacteria and fungi. Choose the antibiotic that is produced by a fungus. a. Streptomycin b. Penicillin c. Chloramphenicol d. Tetracycline b. Penicillin From the data presented in the image the test organism is (antibiotics) a. susceptible to chloramphenicol and penicillin. b. susceptible to chloramphenicol but not penicillin. c. susceptible to penicillin but not chloramphenicol. d. sufficient information is not available to interpret the data. d. sufficient information is not available to interpret the data. In the Kirby Bauer procedure the diameter of the zone is measured and this measurement is compared to a standard chart of Zone sizes. The chart will indicate if the zone size is sufficient for that particular organism is of sufficient size for that organism to be considered susceptible. Data regarding the interpretation of zone sizes is not given. A conclusion cannot be made. In the Kirby Bauer test for antibiotic susceptibility, resistance to an antibiotic is determined from the a. observation of any size of zone around the filter disk. b. observation of a particular size zone, the diameter is measured to determine the size. c. observation of a particular size zone, the radius is measured to determine the size. d. observation of no zone of growth inhibition around the filter disk. b. observation of a particular size zone, the diameter is measured to determine the size. In the Kirby Bauer test for antibiotic susceptibility, the antibiotic to be tested is found in a. the media. b. the filter disks. c. the bacterial sample that is swabbed on the plate. d. the petri plate. b. the filter disks.