Download Table S4

Table S4. Motility and chemotaxis protein-encoding genes
Gene
cheA
cheB
cheC
cheD
cheW
cheY
flgB
flgC
flgE
flhA
flhB
flhF
fliE
fliF
fliG
fliH
fliI
fliJ
fliK
fliL
fliM
fliP
fliQ
fliR
fliY
fliZ
mcpA
mcpB
flhO
flhP
cheR
Signal Ratio (LB + glucose/LB)
WT ccpA ptsH1
hprK
Product
0.42 1.43
0.93
1.11
two-component sensor histidine kinase
0.46 1.40
0.90
1.05
methyl-accepting chemotaxis proteins (MCP)-glutamate
methylesterase and two-component response regulator-like
0.41 1.56
0.97
1.19
inhibition of CheR-mediated methylation of methylaccepting chemotaxis proteins (MCPs) through control of
the binding of CheD to the MCPs
0.51 1.35
0.88
1.32
required for methylation of methyl-accepting chemotaxis
proteins (MCPs) by CheR
0.53 1.24
0.94
1.17
modulation of CheA activity in response to attractants
(chemotaxis)
0.62 1.41
0.72
1.08
two-component response regulator
0.66 1.04
0.71
1.11
flagellar basal-body rod protein
0.61 1.04
0.73
1.07
flagellar basal-body rod protein
0.49 1.35
0.69
0.91
flagellar hook protein
0.53 1.54
0.91
0.98
flagella-associated protein
0.53 1.80
0.89
1.08
flagella-associated protein
0.49 1.68
0.88
1.10
flagella-associated protein
0.46 1.12
0.77
1.04
flagellar hook-basal body protein
0.51 1.10
0.67
1.11
flagellar basal-body M-ring protein
0.53 1.13
0.81
1.06
flagellar motor switch protein
0.49 1.21
0.69
1.06
flagellar assembly protein
0.53 1.34
0.76
1.06
flagellar-specific ATP synthase
0.51 1.18
1.03
0.99
flagellar protein
0.43 1.36
0.59
0.97
flagellar hook-length control
0.41 1.67
0.71
0.93
flagellar protein
0.54 1.48
0.79
1.02
flagellar motor switch protein
0.45 1.82
0.77
1.12
flagellar protein
0.46 1.66
0.81
1.25
flagellar protein
0.48 1.72
0.80
1.14
flagellar protein
0.57 1.42
0.70
1.15
flagellar motor switch protein
0.52 1.63
0.75
1.18
flagellar protein
0.54 1.55
1.01
0.71
methyl-accepting chemotaxis protein
0.66 1.48
0.93
0.89
methyl-accepting chemotaxis protein
0.84 1.30
1.03
1.23
flagellar basal-body rod protein
1.00 1.29
1.06
0.99
flagellar hook-basal body protein
0.99 1.06
0.74
1.50
methyl-accepting chemotaxis proteins (MCPs)
methyltransferase
Rank
CcpA Binding Site
Sequence
Position
473
AGCAAGCGGTATCA
-966
833
TGAAAGCATAACCT
615
632
TGTAAAGGTTTACA
cheV
0.75
1.59
0.92
0.58
mcpC
fliD
fliS
fliT
flgK
flgL
flgM
0.21
0.62
0.57
0.58
0.70
0.70
0.57
1.16
1.42
1.47
1.42
1.31
1.28
1.14
0.73
0.83
0.88
0.91
0.98
0.93
1.11
0.91
0.93
0.97
0.97
0.97
0.79
1.02
hag
motA
motB
0.89
0.75
0.72
1.49
1.89
2.01
1.21
1.09
1.17
0.92
0.58
0.51
Average 0.58
ratio
1.42
0.87
1.02
modulation of CheA activity in response to attractants
(chemotaxis)
methyl-accepting chemotaxis protein
flagellar hook-associated protein 2 (HAP2)
flagellar protein
flagellar protein
flagellar hook-associated protein 1 (HAP1)
flagellar hook-associated protein 3 (HAP3)
anti-sigma factor repressor of sigma-D-dependent
transcription
flagellin protein
motility protein A
motility protein B