Download and only - Phibro Vaccines

Phibro
Vaccines
Phibro
Vaccines
poultry vaccination experts TM
poultry vaccination experts TM
sE.D.S Vaccine
Combination Package
sE.D.S. Monovalent single vaccine
sE.D.S. + N.D.V Bivalent double vaccine
sE.D.S. + N.D.V + I.B Trivalent triple vaccine
Inactivated, oil emulsion vaccines for the protection of Layers and Breeders
against Egg Drop Syndrome, Newcastle Disease and Infectious Bronchitis.
(sE.D.S is a subunit of E.D.S virus. ND component is the V.H. clone of Newcastle disease virus. I.B
component is the M41 virus of infectious bronchitis)
What is the difference between an inactivated, standard whole virus E.D.S
and a sub-unit inactivated vaccine?
The standard vaccine contains the whole E.D.S. virus grown in duck eggs, whereas the sE.D.S.
vaccin contains only the relevant antigen which produces the protective immune response.
Phibro
Vaccines
poultry vaccination experts TM
sE.D.S Vaccine
Why you should prefer the sE.D.S vaccine?
Inactivated Sub-Unit EDS Vaccine
The standard vaccine originating from duck eggs may pose a potential risk of contaminant
pathogens, since there are no SPF Duck eggs available. This risk is avoided when using the subunit vaccine produced in completely safe systems.
How do we monitor the immune response to sE.D.S vaccination?
We recommend to use the HI test. This test is more sensitive and can detect low titers and reduce
false negative results, compared with the ELISA test.
Good perfomance maintained in layers vaccinated with sE.D.S
Layers vaccinated with sE.D.S+N.D.V+I.B maintained good antibody levels throughout production
cycle of observation against ND, I.B and E.D.S.
www.phibro-vaccines.com
1
st and only
subunit EDS vaccine
sE.D.S Vaccination
E. coli expressed Knob proteins
Neutralized E.D.S Virus
Layer type chickens were vaccinated with sE.D.S + N.D.V + I.B at 5 weeks of age. 20 week later - sera were
tested for HI titers and the SN test. The birds were then challenged with 104.6 EID50 of E.D.S 76 virus. Birds
were sacrificed 4 days later. Virus re-isolation was done by using cloacal swabs and spleen homogenates
inoculated into the chorio-allantoic cavity of 10 day old duck embryos.
SPF layers were vaccinated at 16 weeks of age with sE.D.S+N.D.V+I.B and kept under commercial conditions.
The layers were monitored for antibody titers at 2, 3, 4, 5, 6, 8 months post vaccination and for weekly egg
production from 20 weeks of age onward.
A non-vaccinated group of SPF layers served as controls.
Full protection against the E.D.S 76 field virus was shown in vaccinated chickens as demonstrated by the
high antibody level and high neutralization index compared to the un-vaccinated controls. The challenge
virus was completely absent in the vaccinated chickens, whereas it was re-isolated from spleens and cloaca
of the control group.
No. of reisolation of E.D.S from 8 Spleen
and 8 Cloaca 4 days post challenge
HI & SN values of E.D.S Ab in test &
control 20 weeks post vaccination
Vaccinated
Viral Knob protein
8
Vaccinated
Control
E.D.S Vaccinated flock
100%
6
5
4
3
2.37
2
1.2
1
80%
HI
6
5
5
4
3
2
1
1
0
0
70%
7
0
0
SN
Spleen
12
10
10
8
8
6
Exposure to Newcastle disease virus
4
2
2
0
2
3
Cloaca
4
5
6
8
2
N.D.V titers High antibody titers and long duration of
immunity against Newcastle disease virus and protection
from field after a single vaccination with sE.D.S + N.D.V + I.B
compared to non-vaccinated control
10%
HI Values (Log2)
0
25
30
35
40
45
50
12
2000
Age in Weeks
9.44
1600
Egg production was recorded as the percentage of eggs produced calculated each week.
The sE.D.S+N.D.V+I.B vaccinated layers performed as good as the E.D.S+N.D.V+I.B vaccinated controls.
1400
Elisa OD
10.89
10.33
1800
7.80
1200
1000
11.00
1,820
10
1,550
not vaccinated control
1,365
1,246
8
6
814
800
4
600
200
0
10
8
6
4
400
2
0
0
2
3
4
Weeks from vaccination
5
6
0
sE.D.S + N.D.V + I.B vaccinated
12
HI Log 2
20
Elisa titers
Seroconversion
Exposure to Newcastle disease virus
2
0
2
3
4
Month post vaccination
3
4
5
Month post vaccination
Month post vaccination
Monitoring of sE.D.S serological response by HI
Comparison of ELISA OD and HI log2 in sE.D.S vaccinated SPF chickens
20%
sE.D.S + N.D.V + I.B vaccinated
6
4
50%
30%
not vaccinated control
12
60%
40%
I.B titers Protective levels of Infectious
Bronchitis antibodiesare kept for 8 months
after a single vaccination with sE.D.S + N.D.V
+ I.B compared to non-vaccinated controls
sE.D.S + N.D.V + I.B vaccinated
0
0
HI Log2
% of egg production
90%
No. of positive Organ
HI & SN values Log 2
sE.D.S Vaccinated flock
not vaccinated control
Control
8
7.6
7
Full layer production cycle week
E.D.S titers High titers, Long and stable duration of
immunity in the group vaccinated with sE.D.S + N.D.V
+I.B compared to non-vaccinated control
HI Log2
E.D.S Virus
Efficacy of the sE.D.S. + N.D.V + I.B vaccine
2
After an intensive research it was found that the protective antigen of the virus is the tip of the
fiber, known as the “knob”. This protein is expressed in an E. coli expression system.
sE.D.S fully protects chickens against virulent field virus
HI Log
Which part of the E.D.S virus is used in the
sub-unit vaccine?
5
6
8
6
8