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Mr. Kalin Biology 10 Where do heterotrophs get Energy? FOOD: building blocks for growth & life Food molecules contain chemical energy stored in their chemical bonds Carbohydrates, Lipids, & Proteins Energy stored in bonds depends on type of Food Energy Measurement calories = amount of energy needed to raise 1 gram of H2O 1 degree Celsius Calorie = 1,000 calories = 1 kilocalorie 1 gram of glucose = 3811 calories 1 gram of triglyceride = 8893 calories Carbs & Proteins store approx. 4,000 calories/gram Fats store approx. 9,000 calories/gram Analyzing Data pg. 251 Most protein = Eggs, Most carbohydrate = Chocolate, Most fat = Chocolate 2. 2 slices of Bacon = 5g(4Cal) + 6g(9Cal) = 74 Calories, 3 slices of turkey = 11g(4Cal) + 3g(4Cal) + 1g(9Cal) = 65 Calories 3. Chocolate = 3g(4Cal) + 23g(4Cal) + 13g(9Cal) = 221 Calories 1. 1. 221/300 = 44.2 minutes Cell Respiration Cell’s role in making “E” for itself Cellular “E” = ATP Adenosine Tri-Phosphate We eat food (carbos, protein, & lipids) & the mitochondria turn it into ATP Heterotrophs – must eat food to get energy (cannot make their own) Cell Respiration Occurs in the cytoplasm and in the “Mighty” Mitochondria Cell Respiration can occur with or without Oxygen (O2) Aerobic (yields more “E”) Anaerobic (fermentation = less “E”) C6H12O6 + 6O2 6CO2 + 6H2O + “E” (ATP) Where does the “E” come from? C6H12O6 – the bonds between the molecules are where the energy is stored! Cell Respiration is a step-by-step process that breaks the bonds of the glucose and converts the “E” to ATP Structural Formula of Glucose 3 Stages of Cell Respiration Glycolysis Occurs in cytoplasm Splits the glucose in 2 Yields 2 ATP Kreb’s Cycle Breaks glucose completely apart Electron Carriers store bond “E” and take it to E.T.C. Yields 2 ATP Electron Transport Chain Takes bond “E” and makes MAJOR amount of ATP Yields 34 ATP!!! Glycolysis Glyco – sugar Lysis – breaking 2 ATP required to begin process 4 ATP are produced = net gain of 2 ATP Glucose (6C) is broken into 2 pyruvic acids (3C) 2NAD+ + 2NADH (4 e) Fast – can produce thousands of ATP & does not require O2 Kreb’s Cycle Occurs in the matrix (center) of mitochondria Pyruvic Acid is broken down through a series of chemical rx Extracts electrons and some energy and releases CO2 Begins by… 1 C molecule is removed from Pyruvic Acid (CO2), coenzyme A is added Acetyl CoA Joins with 4 C molecule Citric Acid Kreb’s Cycle Continued Citirc Acid is then broken down into a 4 C molecule 2 CO2 molecules are released 1 ATP is produced 8 Electrons are transferred to electron carriers Because glycolysis produces 2 pyruvic acid molecules from each glucose molecule, the Kreb’s Cycle “turns” twice (double the output above) Electron Transport Chain Occurs in the membrane space of mitochondria Products from the Kreb’s and glycolysis feed this cycle Only works in the presence of O2 This chain uses the energy from the “high energy” electrons to turn ADP ATP ETC Continued (Electron Transport) NADH & FADH2 dump their electrons off within the membrane of the mitochondria The ETC contains a series of electron carriers that pass the electrons down to an enzyme that uses their energy to form ATP For every 2 high energy electron that pass down the chain, H+ ions are transferred to the intermembrane space, making it more + compared to the matrix ETC Cont. (ATP Production) Chemiosmosis – process by which a cell produces ATP The H+ ions use the established concentration gradient to move through the enzyme, ATP synthase, an O atom accepts 2 H+ ions and 2 electrons H2O As they move down their gradient, ATP synthase spins, each spin grabs an ADP and joins it with a P to make ATP Each pair of electrons can generate 3 ATP Cell Respiration Totals All Totals per Glucose (C6H12O6) molecule Glycolysis 2 ATP Kreb’s Cycle 2 ATP Electron Transport Chain 32-34 ATP What happens when NO O2 is present? In Animals? Lactic Acid Fermentation Muscle Cramps Why does this happen? NADH builds up but there is no where for the electrons to go! NAD+ needs to be recycled or it will run out Lactate removes the electrons from NADH NAD+ Energy & Exercise 3 sources of muscle energy ATP stored in muscles seconds ATP made from lactic acid fermentation Up to 90 seconds ATP made from cellular respiration Any activity above 90 seconds Prokaryotic Energy Production In Smaller Organisms (bacteria)? Fermentation Milk, Yogurt, Cheese Alcoholic Fermentation Yeasts and a few other organisms Produces ethanol & CO2 Makes spirits & bread Alcoholic Fermentation