Download 2/5.DMD – theory

Division of Studies in English MICROBIOLOGY class content theory
(2/5 DMD – summer semester)
Microbiology sources:
1) Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone Elsevier,
2010;
2) Marsh PD, Martin MV: Oral Microbiology. Churchill Livingstone Elsevier, 2017 (or 2009).
Class 1. General microbiology –
Bacteriological media, methods of the microbiological inoculation and cultivation, preparation of
pure cultures. Physiology of bacteria. The influence of the physical and chemical factors on
bacteria.
Knowledge - The student knows:
- the bacterial physiology, the optimal conditions for their growth in vitro: nutritional requirements
(chemical components of the bacterial cell, various requirements of nutrients); temperature
(psychrophilic bacteria, mesophiles, and thermophiles);
- gaseous requirements of bacteria (strictly aerobic bacteria, facultative anaerobic bacteria, strictly
anaerobic bacteria, microaerophilic bacteria, capnophiles), pH and the osmotic pressure requirements;
- the microbiological media (liquid, semi-solid and solid, simple and enriched, selective, diagnostic
and selective-diagnostic);
- the techniques of the microbiological inoculation, and the methods used to get pure cultures;
- the phases of the bacterial growth;
- the students is able to describe the bacterial growth in the liquid medium (surface growth, turbidity,
and sediment) and in the solid medium (bacterial colony characteristics);
- definitions: resident and transient microflora, carriage of microorganisms; source, reservoir, vector
and ways of transmission of pathogens;
- the following methods: sanitization, asepsis, antisepsis, disinfection and sterilization.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 6 – Diagnostic microbiology and laboratory methods.
Class 2: General microbiology.
Structure of bacterial cell.
Methods of staining.
Knowledge: The student knows:
- the structure of the bacterial cell, shapes and arrangement of bacterial cells under the microscope;
- the staining methods of the bacterial preparations (simple and complex, positive and negative,
positive-negative);
- the types of microscopes used in bacteriology and their application;
- the role of the microscopic slides in the microbiological diagnostics;
- methods of the bacterial identification on the basis of the biochemical features and antigenic
structure of the bacterium.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 6 – Diagnostic microbiology and laboratory methods.
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Division of Studies in English MICROBIOLOGY class content theory
(2/5 DMD – summer semester)
Class no. 3: General microbiology.
Identification of microorganisms according to biochemical and antigenic properties. Sensitivity to
antibiotics testing. Resistance mechanisms.
Knowledge: The student knows:
- the biochemical, serological, and molecular biology methods of identification of microorganisms;
- general characteristics, mechanisms of action of antibiotics and chemotherapeutics;
- the side-effects of the antibiotic therapy; Bacterial resistance to antimicrobial agents - its origin and
the ways of transmission (natural and acquired resistance, vertical and horizontal transmission of drug
resistance);
- standardized techniques determining bacterial susceptibility to antimicrobial agents (antibiogram);
- qualitative tests (disc-diffusion method), quantitative tests (E-test);
- the clinical meaning of MIC, MBC and MBQ.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 7 – Antimicrobial chemotherapy.
Class no. 4: Seminar: Colloquium from the general microbiology (multiple-choice question).
Knowledge: The student knows the way of analyzing bacterial susceptibility results (antibiogram);
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 6 – Diagnostic microbiology and laboratory methods.
Class no. 5:
Microbiology of the Oral Cavity: Microorganisms responsible for infections within the oral
cavity: Genus: Staphylococcus, Streptococcus, Enterococcus.
Knowledge: the student knows:
Taxonomy, reservoir, source, way of transmission, microscopic view, pathogenicity, prevention of
diseases, and identification methods of: Staphylococcus spp., Streptococcus spp., Enterococcus spp.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 11 – Streptococci, staphylococci, micrococci.
Class 6: Microbiology of the Oral Cavity:
Microorganisms responsible for infections within the oral cavity:
Genus: Mycobacterium, Corynebacterium, Actinomyces, Neisseria, Treponema.
Knowledge: the student knows:
Taxonomy, reservoir, source, way of transmission, microscopic view, pathogenicity, prevention of
diseases, and identification methods of: Mycobacterium spp., Corynebacterium spp., Actinomyces spp.,
Neisseria spp., Treponema spp.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010;
Chapter 12 – Lactobacilli, corynebacteria and propionibacteria.
Chapter 13 – Actinomycetes, clostridia and Bacillus species.
Chapter 14 – Neisseriaceae, Veilonella, parvobacteria, Capnocytophaga.
Chapter 19 – Mycobacteria and legionellae.
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Division of Studies in English MICROBIOLOGY class content theory
(2/5 DMD – summer semester)
Class 7: Seminar: Enterobacteriaceae and non-fermenting rods. Maintaining hygiene at the
dentist.
Knowledge: The student knows: species, reservoir, source, way of transmission, microscopic view,
pathogenicity, prevention of diseases, and identification methods of: Escherichia coli, Klebsiella
pneumoniae, Proteus spp., Salmonella spp., Shigella spp., Pseudomonas spp., Vibrio spp.
The meaning of the systematic hand washing technique of Alliffe, use of gloves, surgery disinfection,
decontamination and sterilization of instruments.
Source: Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone
Elsevier, 2010; Chapter 15 –Enterobacteria.
Elsevier, 2010; Chapter 16 –Vibrios, campylobacters and Wolinella.
Class 8: Microbiology of the Oral Cavity: Natural microbiota of the oral cavity.
Knowledge: the student knows: the physiological flora of the oral cavity and its protective role against
pathogens.
Source:
1) Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone Elsevier,
2010; Chapter 31 –Normal oral flora, the oral ecosystem and plaque biofilms.
2) Marsh PD, Martin MV: Oral Microbiology. Churchill Livingstone Elsevier, 2017 (or 2009).
Chapter 2 – The mouth as a microbial habitat.
Chapter 3 – The resident oral microflora.
Chapter 4 - Acquisition, adherence, distribution and metabolism of the oral microflora.
Chapter 5 – Dental plaque.
Class 9: Microbiology of the Oral Cavity:
Bacteriology of dental caries and periodontal diseases.
Knowledge: the student knows:
- the dental pellicle and plaque - stages of the development, composition, pathologic influence on the
tooth tissue and periodontal tissues;
- bacteria responsible for periodontal diseases and gingivitis; anaerobic bacteria (Bacteroides,
Prevotella, Porpyromonas, Fusobacterium, Clostridium).
1) Lakshman Samaranayake: Essential Microbiology for Dentistry. Churchill Livingstone Elsevier,
2010;
Chapter 17 – Bacteroides, Tanerella, Porphyromonas and Prevotella.
Chapter 18 – Fusobacteria, Leptotricha, and spirochetes.
Chapter 32 – Microbiology of dental caries.
Chapter 33 – Microbiology of periodontal disease.
2) Marsh PD, Martin MV: Oral Microbiology. Churchill Livingstone Elsevier, 2017 (or 2009).
Chapter 2 – Plaque-mediated diseases – dental caries and periodontal diseases.
Class no. 10: Seminar: The practical colloquium from oral microbiology.
Class no. 11: Seminar : Colloquium from the oral microbiology (written ).
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