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Laboratory Techniques Agriculture, Food, and Natural Resource Standards Addressed • AS.07.01. Design programs to prevent animal diseases, parasites and other disorders and ensure animal welfare. – AS.07.01.01.a. Identify and summarize specific tools and technology used in animal health management. – AS.07.01.02.a. Explain methods of determining animal health and disorders. Laboratory Techniques used to diagnose and treat many animal health problems Laboratory Techniques performed in veterinary hospitals, zoos, research facilities, and commercial labs Laboratory Techniques In most cases, it is the vet tech that is responsible for collecting samples and performing the tests. Laboratory Techniques The vet tech must: have a good understanding of proper collection techniques and specimen handling Laboratory Techniques The vet tech must: have a good understanding of use complex equipment Laboratory Techniques The vet tech must: be able to accurately perform the tests since the veterinarian will base treatment on the results Laboratory Techniques - hematology urinalysis susceptibility testing the circulatory and excretory systems The Urinary System consists of: - the kidneys - the ureters - the urinary bladder - the urethra The Urinary System function: - to extract and remove waste from the blood Urinary System Urethra Ureter Kidney Bladder Urinary System Urethra Ureter Kidney Bladder The Urinary System The Kidneys - paired organs located on both sides of the spinal column and are bean-shaped in most species of animal (cattle have several lobes) The Urinary System The Kidneys - responsible for extracting and collecting waste The Urinary System The Kidneys consist of: - cortex - medulla - renal pelvis The Kidney Renal artery Cortex Medulla Renal pelvis Ureter Renal capsule The Urinary System The Kidneys nephrons are directly responsible for the filtering and collection of wastes from the blood (found throughout the cortex and medulla) The Glomerulus Nephron Arterioles Bowman’s capsule Proximal convoluted tubule Distal convoluted tubule Loop of Henle Collecting duct The Urinary System The Kidneys Collecting ducts run through the medulla and drain into the renal pelvis. The Urinary System Urine then passes into the ureters and proceeds to the bladder. The Urinary System - The ureters are smooth muscled tubes that extend from each kidney. - They use peristalsis to move urine to the bladder. The Urinary System Urine is pushed into the bladder every 10 to 30 seconds depending on the species. It flows in spurts rather than continuously. The Urinary System The Bladder - consists of smooth muscle - extremely elastic sac that is capable of holding large volumes of The Urinary System The Bladder - has three openings: - twothat receive urine from the ureters - one used to excrete urine to the urethra The Urinary System The Urethra - carries urine from the bladder to the exterior The Urinary System The Urethra - varies in length and circumference in males and females The Urinary System The Urethra - The female urethra is shorter in length and runs directly from the bladder to the exterior. The Urinary System The Urethra - Males have a narrower urethra that is longer to extend through the accessory sex glands and exit through the penis. The Urinary System The Urethra - Males have a narrower urethra that is longer to extend through the accessory sex glands and exit through the penis. The Urinary System 3 Phases of Urine Production: 1.Filtration 2.Reabsorption 3.Secretion The Urinary System 3 Phases of Urine Production: occur in the nephrons The Urinary System 3 Phases of Urine Production: 1. Filtration • blood enters the glomerulus through the afferent arteriole The Urinary System 3 Phases of Urine Production: 1. Filtration • under various pressures, water, salt, and small molecules move out of the glomerulus and into The Urinary System 3 Phases of Urine Production: 2. Reabsorption • occurs in the proximal convoluted tubule and the loop of Henle The Urinary System 3 Phases of Urine Production: 2. Reabsorption • substances needed by the body such as water and electrolytes will be reabsorbed by the body from the loop of The Urinary System 3 Phases of Urine Production: 3. Secretion • substances are secreted into the collecting tubules and transported to the renal pelvis