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Transcript 
The Effects of Antibiotics on Gastrointestinal
Motility and Gut Microbiota
Catherine Chen
Illinois Mathematics and Science Academy
April 28, 2016
Possible Perturbations of Gastrointestinal Tract
Host Physiology
Microbial Metabolites/
Metabolic Activity
Serotonin Pathway
Bacterial Community
SCFA Production
Hypothesis
Antibiotic use affects GI motility
through alterations in gut microbial
community and/or changes in the host
physiology.
Gut microbiota
GI motility
Goals
1. Determine effects of antibiotics on intestinal
transit time and host physiology.
2. Examine antibiotic effects on microbial
structure and metabolic profile.
Antibiotic usage
Antibiotic
Target
Treats
Cefoperazone
gram +
gram -
infections:
peritonitis
Vancomycin
gram +
diarrhea causing bacteria
Neomycin
gram-
infections,
used in ointments
Study Design
-Abx in drinking water
+Cef in drinking water
+Vanco in drinking water
+Neo in drinking water
Measured Transit time
Collected luminal contents
16s
SCFA
Tissues
mRNA (qRT-PCR)
Gastrointestinal Transit Time
Bacterial Community Changes in AntibioticTreated Luminal Contents
Short Chain Fatty Acid Changes in AntibioticTreated Luminal Contents
Propionate
Butyrate
Acetate
Grider and Piland. AJP. 2007.
Short Chain Fatty Acid Changes in AntibioticTreated Luminal Contents
Antibiotic Treatment Effects on
Gene Expression Levels
Antibiotic Treatment Effects on
Gene Expression Levels
Antibiotic Treatment Effects on
Gene Expression Levels
Antibiotic Treatment Effects on
Gene Expression Levels
Conclusions
Antibiotic treatment can significantly:
•increase GI transit time
•alter intestinal microbial community.
•decrease SCFA production
•affect serotonin pathway
Summary
Microbial metabolite production
Bacterial community structure
GI motility
Gut microbiota
Delayed Transit Time
Constipation
Acknowledgements
• Dr. Chang, Dr. Touw, & the rest of the Chang
lab at University of Chicago
• All the staff of the Student Inquiry Research
Program at the Illinois Mathematics and
Science Academy
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