Download Suppression of cancer cell proliferation by a combination of light

Pharmaceuticals 03
(Keywords: Light-emitting diode (LED), D-Allose, Cancer cells, Light therapy)
●Research representative
Kensho Okamoto, Professor, Kagawa University Faculty of Engineering
Outline of technology
Absorbance
Sales points
●Proliferation of cancer cells can be suppressed effectively by a combination of LED irradiation and administration
of a rare sugar, both of which have few adverse effects and are minimally invasive.
Expected application fields and products
(1)
(2)
(3)
(4)
Treatment
Treatment
Treatment
Treatment
for
for
for
for
skin cancer
leukemia
esophageal, stomach, and colon cancers
lung and laryngeal cancers
(5) Treatment for penile, vaginal, and uterine cancers
(6) Treatment for liver, pancreatic, and ovarian cancers
(7) Treatment for diseases due to abnormal cell
proliferation
Comparison with existing treatments
Advantages
LED irradiation + D-Allose administration
Radiotherapy
Disadvantages
Therapeutic effect
Stress to patients
Hemorrhage, etc.
Cost
No pain
No hemorrhage
Inexpensive
Effective only for early cancers
Inexpensive
Causes immunosuppression
Adverse effects and wasting No hemorrhage Involves risk
Chemotherapy
Adverse effects
No hemorrhage
Expensive
Symptomatic therapy
Surgery
Physically painful
Causes hemorrhage
Expensive
Effective if successful
References, patents, etc.
●Related industrial property rights: Kokai number (2005) 65977 [Application for evaluation have been made]
Other matters to note
(Developer’
s comment)
While this study was an experiment of in vitro nature, it showed that cancer cell proliferation can be suppressed by
the addition of a trace amount of D-allose to the medium and irradiating the medium with blue LED in cancer cell
cultures (liver cancer and leukemia cells). However, the cell growth inhibiting effect of blue LED light was slightly
reduced by the addition of D-allose in some experiments.
(Developer’
s contact address)
TEL: +81-87-864-2250 E-mail: [email protected]
Kagawa Prefecture Glyco-Biocluster Formation Project Collection of Technological Achievements Related to Rare Sugars
Cell count 200 cells/μl
Rare sugar 50 mmol/l
The growth of cancer cells can be
inhibited by both the light of highbrightness light-emitting diode (LED)
96-well plate for MTT
(particularly blue and green lights) and
the rare sugar D-allose alone, but it can
LED light irradiation
be inhibited more effectively by their
concomitant use. Since both treatments
Measurement
Day 1
Day 2
Day 3
Culturing in an incubator
have few adverse effects and are
Time
course
minimally invasive to patients, the
Figure: Changes in the cell count when D-allose was added in a very
combination has a great potential as a
small amount to the culture medium of K562 chronic
new treatment for cancer.
myelocytic leukemia cells and irradiated with blue LED light
Cancers on the surface such as skin
(5W/m2) (proportionate to changes in the absorbance)
cancer can be directly irradiated with
LED. Blood cancers such as leukemia can be exposed to LED by leading blood temporarily out of the body as in
hemodialysis. Cancers of the digestive, respiratory, and reproductive systems such as stomach cancer, lung cancer,
and uterine cancer may be irradiated by LED using an LED irradiator attached to various fiberscopes.
Intraperitoneal cancers such as liver cancer can be irradiated using a laparoscope mounted with an LED irradiator.
D-Allose can be administered by injection, intravenous injection, instillation, topical spraying, or external application
as a solution. This treatment is also considered to be effective for the treatment of diseases due to abnormal
proliferation of cells other than cancer (such as psoriasis vulgaris and verruca).
Glyco-Bio Project Team, Technical Promotion Division, Kagawa Industry Support Foundation 1st floor, FROM Kagawa, 2217-16 Hayashi-cho, Takamatsu City, Kagawa 761-0301 Japan TEL: +81-87-840-0338 FAX: +81-87-864-6303 URL http://www.kagawa-isf.jp/
Suppression of cancer cell proliferation by a combination of lightemitting diode (LED) irradiation and administration of rare sugars
●Research theme