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UHPLC Kolon bilgileri, Uygulama Alanları ve Yapılan Analizler (HPLC Kolonu, Standardı ve IAK Kolonu Mevcut Olan) Mevcut UHPLC Kolon Bilgileri Sıra Adı 1 Hypersil GOLD 2 Hypersil GOLD Amino 3 ODS HYPERSIL (RP C18) Dionex Acclaim 120 4 Thermo /Dionex Kod 25003154630 25705154630 30105254630 059133 Boy mm Çap mm Dolgu µm 150 4.6 150 Standardı Olan (Yapılan Analizler) Talebe Göre Standardı Alınması Planlanan Analizler/ Uygulamalar Aflatoksin (G2G1B2B1) sıra 2 PAH, Aflatoksin M, Okratoksin A, Benzoik asit, Sorbik asit Balda Şeker 120 sıra 3 Balda Hidroksimetilfurfural(HMF) 120 sıra 4 Kafein Gözenek °A Literatür Uygulama 3 175 sıra 1 4.6 5 175 250 4.6 5 150 4.6 3 Talebe Göre Temin Edilebilecek Olan UHPLC Kolon Bilgileri Sıra Adı Boy (mm) Çap (mm) Dolgu µm Literatürdeki Uygulamalar 5 Hypersil GOLD C8 150 4.6 5 sıra 5 6 Hypersil GOLD C4 150 4.6 5 sıra 6 7 Hypersil GOLD aQ 150 4.6 5 sıra 7 8 Hypersil GOLD PFP 150 4.6 5 sıra 8 9 Hypersil GOLD CN 150 4.6 5 sıra 9 10 Hypersil GOLD Phenyl 150 4.6 5 sıra 10 11 Hypersil GOLD AX 150 4.6 5 sıra 11 12 Hypersil GOLD SAX 150 4.6 5 sıra 12 13 Hypersil GOLD Silica 150 4.6 5 sıra 13 14 Hypersil GOLD HILIC 150 4.6 5 sıra 14 Uygulamaya göre seçilebilecek olan diğer kolonlar; https://tools.thermofisher.com/content/sfs/brochures/BRCCSGOLDTECHV4_0910_final290910.pdf http://www.dionex.com/en-us/webdocs/4139-DS_Acclaim_120_LPN1413-02.pdf Kolonların yer aldığı örnek uygulamalar; 1- Hypersil GOLD; Polycyclic aromatic hydrocarbons; Naphthalene, Fluorene, Phenanthrene, Anthracene, Pyrene, Chrysene Food safety: Mycotoxins ; Aflatoksin G2 G1 B2 B1 M, Ochratoxin A Environmental: Banned aromatic amines; 2,4-Diaminotoluene, 4,4-Oxydianiline, o-Toluidine, 2-Methoxy-5-methylaniline, 2,4,5-Trimethylaniline, 4,4'Methylene-bis (2-chloroaniline) Environmental: Endocrine disruptors; Desethyl atrazine, Estriol, Simazine, Atrazine, Diuron, Bisphenol A, Estrone Food safety: Energy drink additives; Acesulfame, Saccharin, Caffeine, Benzoic acid, Sorbic acid, Aspartame Food safety: Tocopherols; δ-Tocopherol, γ-Tocopherol , α-Tocopherol Food safety: Coumaric acids; Uracil, p-Coumaric Acid, m-Coumaric Acid, o-Coumaric Acid Pharmaceutical: Cepha antibiotics; Cefadroxil, Cefaclor, Cephalexin, Cephradine, Cefazolin, Pharmaceutical: Cough / cold formulation; 4-Amino phenol, (chlorpheniramine) maleate, Phenylephrine, Acetaminophen, Saccharin, 4-Nitro phenol, Chlorpheniramine Chemistry Application; Urasil, 2-Acetaminophen, N-Acetyl procainamide, n-Propionyl procainamide, Oxazepam, Diazepam, Diisopropylphthalate, Di-npropylphthalate Anaesthetics; Lidocaine, Tetracaine, Benzocaine, Clinical: Testosterones; 11-Ketotestosterone, 19-Nortestosterone (nandrolone), Testosterone, Epitestosterone Clinical: Chlorpromazine; Chlorpromazine Clinical: Tricyclic antidepressants; Doxepin, Protriptyline, Imipramine, Nortriptyline, Amitriptyline, Trimipramine 1) Determination of Theanine, GABA, and Other Amino Acids in Green, Oolong, Black, and Pu-erh Teas with Dabsylation and High-Performance Liquid Chromatography 2) Novel Water-Soluble Near-Infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes 3) Early life progestin exposure causes arrested oocyte development, oviductal agenesis and sterility in adult Xenopus tropicalis frogs 4) Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum 5) HPLC Organic Acid Analysis in Different Citrus Juices under Reversed Phase Conditions 6) Simultaneous Determination of Sudan Dyes and Carotenoids in Red Pepper and Tomato Products by HPLC 2- Hypersil GOLD Amino Food Safety: Sugars; Fructose, Glucose, Sucrose, Maltose, Lactose Food Safety: Sorbitol 1) Synthesis of a molecularly imprinted polymer and its application for microextraction by packed sorbent for the determination of fluoroquinolone related compounds in water 2) Tissue distributions and seasonal dynamics of the hepatotoxic microcystins-LR and -RR in a freshwater snail (Bellamya aeruginosa) from a large shallow, eutrophic lake of the subtropical China 3) Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production 4) Synthesis and post-synthetic derivatization of a cyanine-based amino acid. Application to the preparation of a novel water-soluble NIR dye 5) Stability-indicating hydrophilic interaction liquid chromatography method for highly polar and basic compounds 6) Retention prediction of a set of amino acids under gradient elution conditions in hydrophilic interaction liquid chromatography 3- ODS Hypersil (Octadecylsilane Reverse Phase C18 Column) 1) Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C18 column and pyridine-containing mobile phases 2) Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants 3) Determination of mercury species in natural waters at picogram level with on-line RP C18 preconcentration and HPLC-UV-PCO-CVAAS 4) Radioenzymic determination of homocysteine in plasma and urine. 5) Determination of the levels of abscisic acid-glucose ester in plants 6) Comparative study of the reversed-phase high-performance liquid chromatography of black tea liquors with special reference to the thearubigins 7) Distribution of Tamoxifen and Its Metabolites in Rat and Human Tissues during Steady-State Treatment 8) Determination of column selectivity toward polycyclic aromatic hydrocarbons 9) Structural analysis of the O-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens 10) B and C terms in the Van Deemter equation for liquid chromatography 11) Isocratic Separation of PTH-Amino Acids at Picomole Level by Reverse-Phase HPLC in the Presence of Sodium Dodecylsulfate 12) tRNA separation by high-performance liquid chromatography using an aggregate of ods-hypersil and trioctyl-methylammonium chloride 4- Silica C18 (Dionex Bonded) Food and safety; Caffeine 1) Determination of the degrees of methylation and acetylation of pectins using a C18 column and internal standards 5- Hypersil GOLD C8 Food safety: Fatty acids: Linoleic Acid Environmental: Triazines and uron herbicides; Simazine, Monuron, Chlorotoluron, Atrazine, Diuron, Propazine, Linuron Food safety: b-Carotene; Lutein, Lycopene, β-Carotene Environmental: Phthalates; Dimethyl phthalate, Diethyl phthalate, Dipropyl phthalate, Diisopropyl phthalate, Di-n-butyl phthalate, Di-n-octyl phthalate 1) Short-incubation mass spectrometry assay for lysosomal storage disorders in newborn and high-risk population screening 6- Hypersil GOLD C4 Pharmaceutical: Parabens; Methylparaben, Ethylparaben, Propylparaben, Butylparaben Food safety: Fatty Acids; Linolenic acid, Linoleic acid 7- Hypersil GOLD aQ Food safety: Water soluble vitamins ; Vitamin B1 (thiamine), Vitamin B6 (pyridoxine), Vitamin B3 (nicotinamde), Vitamin B5 (pantothenic acid), Folic Acid, Vitamin B12 (cyanocobalamin), Vitamin H (biotin), Vitamin B2 (riboflavin) Pharmaceutical: Sulfonamides; Sulfaguanidine, Sulfanilamide, Sulfathiazole, Sulfamerazine, Sulfamonomethoxine, Sulfaquinoxaline Biochemical: PTH amino acids; Serine, Asparagine, Aspartic acid, Glutamic acid, Alanine, Tyrosine, Methionine, Tryptophan, Phenylanaline, Leucine Pharmaceutical: Xanthines ; Hypoxanthine, Xanthine, Theobromine, Theophylline, Caffeine 1) Organic Acid Analysis in Different Citrus Juices under Reversed Phase Conditions 2) Development of a HPLC–UV method for the quantitative determination of four short-chain fatty acids and lactic acid produced by intestinal bacteria during in vitro fermentation 3) Chromatographic resolution of closely related species: Separation of warfarin and hydroxylated isomers 4) Accumulation of perfluoroalkyl substances in human tissues 8- Hypersil GOLD PFP Pharmaceutical: Taxanes; 10 -Deacetyl baccatin, Baccatin III, 10-Deacetyl-7-xylosyl taxol B, Taxinine M, 10-Deacetyl-7-xylosyl taxol, 10-Deacetyl taxol, 10Deacetyl-7-xylosyl taxol C, 7-Xylosyl taxol, Cephalomanine, 10-Deacetyl-7epitaxol, Paclitaxol, Taxol C, 7-Epitaxol Environmental: Banned aromatic amines; 2,4-Diaminotoluene, o-Toluidine, 4,4-Oxydianiline, 2-Methoxy-5-Methylaniline, 2,4,5-Trimethylaniline, 4,4Methylene-bis (2-chloroaniline) Environmental: Phenolic positional isomers ; 3,4-Dimethoxyphenol, 6-Dimethoxyphenol, 2,6-Difluorophenol, 3,5-Dimethoxyphenol, 2,4-Difluorophenol, 2,3Difluorophenol, 3,4-Difluorophenol, 3,5-Dimethoxyphenol, 2,6-Dimethoxyphenol, 2,6-Dichlorophenol, 4-Chloro-3-Methylphenol 3,4-Dichlorophenol, 4-Chloro-2Methylphenol, 3,5-Dichlorophenol Pharmaceutical: Fluorinated nucleic bases; Fluorocytosine, Fluorouracil 9- Hypersil GOLD CN Clinical: Steroids; Hydrocortisone, Cortisone, Corticosterone, 11-α Hydroxprogesterone, 17-α Hydroxprogesterone, Progesterone Deoxycorticosterone Pharmaceutical: Penicillins; N-acetyl Penicillamine, Ampicillin, Penicillin G Pharmaceutical: TB Drugs; Isoniazid, Pyrazinamide General: Organic acids; 4-Fluorobenzoic, o-Toluic Acid, p-Toluic Acid, 2,4,6-Trimethylbenzoic Acid, 2,5-Dimethylbenzoic Acid 10-Hypersil GOLD Phenyl Pharmaceutical: Antibacterials; Carbadox, Thiamphenicol, Furazolidone, Oxolinic Acid, Sulfadimethoxine, Sulfaquinoxaline, Nalidixic Acid, Piromidic Acid Pharmaceutical: Antacids ; Famotidine, Cimetidine, Ranitidine Pharmaceutical: Veterinary drug coccidiostats; 4-amino-3,5-dinitrobenzamide, Zoalene (3,5-nitro-o-toluamide, Nitromid (3,5-dinitrobenzamide), Ethopabate Pharmaceutical: Antidepressants; Uracil, Acetaminophen, p-Hydroxybenzoic acid, o-Hydroxybenzoic acid, Oxazepam, Diazepam, Di-isopropyl phthalate, Di-npropyl phthalate 11-Hypersil GOLD AX Biotech: Monophosphates Food safety: Vitamin C 12-Hypersil GOLD SAX Monophosphates; Uracil, Cytidine-5’-monophosphate, Adenosine-5’-monophosphate, Guanosine-5’-monophosphate 13- Hypersil GOLD Silica Forensics: Steroids ; Progesterone, 21-Hydroxyprogesterone-21-acetate, 17-a-Hydroxyprogesterone, Cortisone, 11-a-Hydroxyprogesterone, Corticosterone, Hydrocortisone 14- Hypersil GOLD HILIC Food Safety: Water soluble vitamins; Thiamine, Nicotinic acid, Nicotinamide, Pyridoxine, Riboflavin, PABA. Clinic: Urea Kaynak; https://tools.thermofisher.com/content/sfs/brochures/BRCCSGOLDTECHV4_0910_final290910.pdf http://www.dionex.com/en-us/webdocs/4139-DS_Acclaim_120_LPN1413-02.pdf https://www.thermofisher.com/tr/en/home/applications-techniques.html http://www.dionex.com/en-us/documents/application-notes-updates/lp-87103.html http://www.jascoinc.com/chromatography/uhplc http://www.sielc.com/Applications_By_Compound.html