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UHPLC Kolon bilgileri, Uygulama Alanları ve Yapılan Analizler (HPLC Kolonu, Standardı ve IAK Kolonu Mevcut Olan)
Mevcut UHPLC Kolon Bilgileri
Sıra
Adı
1
Hypersil GOLD
2
Hypersil GOLD Amino
3
ODS HYPERSIL
(RP C18)
Dionex Acclaim 120
4
Thermo
/Dionex
Kod
25003154630
25705154630
30105254630
059133
Boy
mm
Çap
mm
Dolgu
µm
150
4.6
150
Standardı Olan
(Yapılan Analizler)
Talebe Göre Standardı Alınması
Planlanan Analizler/ Uygulamalar
Aflatoksin
(G2G1B2B1)
sıra 2
PAH, Aflatoksin M, Okratoksin A,
Benzoik asit, Sorbik asit
Balda Şeker
120
sıra 3
Balda Hidroksimetilfurfural(HMF)
120
sıra 4
Kafein
Gözenek
°A
Literatür
Uygulama
3
175
sıra 1
4.6
5
175
250
4.6
5
150
4.6
3
Talebe Göre Temin Edilebilecek Olan UHPLC Kolon Bilgileri
Sıra
Adı
Boy (mm)
Çap (mm)
Dolgu µm
Literatürdeki Uygulamalar
5
Hypersil GOLD C8
150
4.6
5
sıra 5
6
Hypersil GOLD C4
150
4.6
5
sıra 6
7
Hypersil GOLD aQ
150
4.6
5
sıra 7
8
Hypersil GOLD PFP
150
4.6
5
sıra 8
9
Hypersil GOLD CN
150
4.6
5
sıra 9
10
Hypersil GOLD Phenyl
150
4.6
5
sıra 10
11
Hypersil GOLD AX
150
4.6
5
sıra 11
12
Hypersil GOLD SAX
150
4.6
5
sıra 12
13
Hypersil GOLD Silica
150
4.6
5
sıra 13
14
Hypersil GOLD HILIC
150
4.6
5
sıra 14
Uygulamaya göre seçilebilecek olan diğer kolonlar;
https://tools.thermofisher.com/content/sfs/brochures/BRCCSGOLDTECHV4_0910_final290910.pdf
http://www.dionex.com/en-us/webdocs/4139-DS_Acclaim_120_LPN1413-02.pdf
Kolonların yer aldığı örnek uygulamalar;
1- Hypersil GOLD;
Polycyclic aromatic hydrocarbons; Naphthalene, Fluorene, Phenanthrene, Anthracene, Pyrene, Chrysene
Food safety: Mycotoxins ; Aflatoksin G2 G1 B2 B1 M, Ochratoxin A
Environmental: Banned aromatic amines; 2,4-Diaminotoluene, 4,4-Oxydianiline, o-Toluidine, 2-Methoxy-5-methylaniline, 2,4,5-Trimethylaniline, 4,4'Methylene-bis (2-chloroaniline)
Environmental: Endocrine disruptors; Desethyl atrazine, Estriol, Simazine, Atrazine, Diuron, Bisphenol A, Estrone
Food safety: Energy drink additives; Acesulfame, Saccharin, Caffeine, Benzoic acid, Sorbic acid, Aspartame
Food safety: Tocopherols; δ-Tocopherol, γ-Tocopherol , α-Tocopherol
Food safety: Coumaric acids; Uracil, p-Coumaric Acid, m-Coumaric Acid, o-Coumaric Acid
Pharmaceutical: Cepha antibiotics; Cefadroxil, Cefaclor, Cephalexin, Cephradine, Cefazolin,
Pharmaceutical: Cough / cold formulation; 4-Amino phenol, (chlorpheniramine) maleate, Phenylephrine, Acetaminophen, Saccharin, 4-Nitro phenol,
Chlorpheniramine
Chemistry Application; Urasil, 2-Acetaminophen, N-Acetyl procainamide, n-Propionyl procainamide, Oxazepam, Diazepam, Diisopropylphthalate, Di-npropylphthalate
Anaesthetics; Lidocaine, Tetracaine, Benzocaine,
Clinical: Testosterones; 11-Ketotestosterone, 19-Nortestosterone (nandrolone), Testosterone, Epitestosterone
Clinical: Chlorpromazine; Chlorpromazine
Clinical: Tricyclic antidepressants; Doxepin, Protriptyline, Imipramine, Nortriptyline, Amitriptyline, Trimipramine
1) Determination of Theanine, GABA, and Other Amino Acids in Green, Oolong, Black, and Pu-erh Teas with Dabsylation and High-Performance Liquid
Chromatography
2) Novel Water-Soluble Near-Infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes
3) Early life progestin exposure causes arrested oocyte development, oviductal agenesis and sterility in adult Xenopus tropicalis frogs
4) Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum
5) HPLC Organic Acid Analysis in Different Citrus Juices under Reversed Phase Conditions
6) Simultaneous Determination of Sudan Dyes and Carotenoids in Red Pepper and Tomato Products by HPLC
2- Hypersil GOLD Amino
Food Safety: Sugars; Fructose, Glucose, Sucrose, Maltose, Lactose
Food Safety: Sorbitol
1) Synthesis of a molecularly imprinted polymer and its application for microextraction by packed sorbent for the determination of fluoroquinolone related
compounds in water
2) Tissue distributions and seasonal dynamics of the hepatotoxic microcystins-LR and -RR in a freshwater snail (Bellamya aeruginosa) from a large shallow,
eutrophic lake of the subtropical China
3) Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production
4) Synthesis and post-synthetic derivatization of a cyanine-based amino acid. Application to the preparation of a novel water-soluble NIR dye
5) Stability-indicating hydrophilic interaction liquid chromatography method for highly polar and basic compounds
6) Retention prediction of a set of amino acids under gradient elution conditions in hydrophilic interaction liquid chromatography
3- ODS Hypersil (Octadecylsilane Reverse Phase C18 Column)
1) Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C18 column and pyridine-containing
mobile phases
2) Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus
officinalis plants
3) Determination of mercury species in natural waters at picogram level with on-line RP C18 preconcentration and HPLC-UV-PCO-CVAAS
4) Radioenzymic determination of homocysteine in plasma and urine.
5) Determination of the levels of abscisic acid-glucose ester in plants
6) Comparative study of the reversed-phase high-performance liquid chromatography of black tea liquors with special reference to the thearubigins
7) Distribution of Tamoxifen and Its Metabolites in Rat and Human Tissues during Steady-State Treatment
8) Determination of column selectivity toward polycyclic aromatic hydrocarbons
9) Structural analysis of the O-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens
10) B and C terms in the Van Deemter equation for liquid chromatography
11) Isocratic Separation of PTH-Amino Acids at Picomole Level by Reverse-Phase HPLC in the Presence of Sodium Dodecylsulfate
12) tRNA separation by high-performance liquid chromatography using an aggregate of ods-hypersil and trioctyl-methylammonium chloride
4- Silica C18 (Dionex Bonded)
Food and safety; Caffeine
1) Determination of the degrees of methylation and acetylation of pectins using a C18 column and internal standards
5- Hypersil GOLD C8
Food safety: Fatty acids: Linoleic Acid
Environmental: Triazines and uron herbicides; Simazine, Monuron, Chlorotoluron, Atrazine, Diuron, Propazine, Linuron
Food safety: b-Carotene; Lutein, Lycopene, β-Carotene
Environmental: Phthalates; Dimethyl phthalate, Diethyl phthalate, Dipropyl phthalate, Diisopropyl phthalate, Di-n-butyl phthalate, Di-n-octyl phthalate
1) Short-incubation mass spectrometry assay for lysosomal storage disorders in newborn and high-risk population screening
6- Hypersil GOLD C4
Pharmaceutical: Parabens; Methylparaben, Ethylparaben, Propylparaben, Butylparaben
Food safety: Fatty Acids; Linolenic acid, Linoleic acid
7- Hypersil GOLD aQ
Food safety: Water soluble vitamins ; Vitamin B1 (thiamine), Vitamin B6 (pyridoxine), Vitamin B3 (nicotinamde), Vitamin B5 (pantothenic acid), Folic Acid,
Vitamin B12 (cyanocobalamin), Vitamin H (biotin), Vitamin B2 (riboflavin)
Pharmaceutical: Sulfonamides; Sulfaguanidine, Sulfanilamide, Sulfathiazole, Sulfamerazine, Sulfamonomethoxine, Sulfaquinoxaline
Biochemical: PTH amino acids; Serine, Asparagine, Aspartic acid, Glutamic acid, Alanine, Tyrosine, Methionine, Tryptophan, Phenylanaline, Leucine
Pharmaceutical: Xanthines ; Hypoxanthine, Xanthine, Theobromine, Theophylline, Caffeine
1) Organic Acid Analysis in Different Citrus Juices under Reversed Phase Conditions
2) Development of a HPLC–UV method for the quantitative determination of four short-chain fatty acids and lactic acid produced by intestinal bacteria during
in vitro fermentation
3) Chromatographic resolution of closely related species: Separation of warfarin and hydroxylated isomers
4) Accumulation of perfluoroalkyl substances in human tissues
8- Hypersil GOLD PFP
Pharmaceutical: Taxanes; 10 -Deacetyl baccatin, Baccatin III, 10-Deacetyl-7-xylosyl taxol B, Taxinine M, 10-Deacetyl-7-xylosyl taxol, 10-Deacetyl taxol, 10Deacetyl-7-xylosyl taxol C, 7-Xylosyl taxol, Cephalomanine, 10-Deacetyl-7epitaxol, Paclitaxol, Taxol C, 7-Epitaxol
Environmental: Banned aromatic amines; 2,4-Diaminotoluene, o-Toluidine, 4,4-Oxydianiline, 2-Methoxy-5-Methylaniline, 2,4,5-Trimethylaniline, 4,4Methylene-bis (2-chloroaniline)
Environmental: Phenolic positional isomers ; 3,4-Dimethoxyphenol, 6-Dimethoxyphenol, 2,6-Difluorophenol, 3,5-Dimethoxyphenol, 2,4-Difluorophenol, 2,3Difluorophenol, 3,4-Difluorophenol, 3,5-Dimethoxyphenol, 2,6-Dimethoxyphenol, 2,6-Dichlorophenol, 4-Chloro-3-Methylphenol 3,4-Dichlorophenol, 4-Chloro-2Methylphenol, 3,5-Dichlorophenol
Pharmaceutical: Fluorinated nucleic bases; Fluorocytosine, Fluorouracil
9- Hypersil GOLD CN
Clinical: Steroids; Hydrocortisone, Cortisone, Corticosterone, 11-α Hydroxprogesterone, 17-α Hydroxprogesterone, Progesterone Deoxycorticosterone
Pharmaceutical: Penicillins; N-acetyl Penicillamine, Ampicillin, Penicillin G
Pharmaceutical: TB Drugs; Isoniazid, Pyrazinamide
General: Organic acids; 4-Fluorobenzoic, o-Toluic Acid, p-Toluic Acid, 2,4,6-Trimethylbenzoic Acid, 2,5-Dimethylbenzoic Acid
10-Hypersil GOLD Phenyl
Pharmaceutical: Antibacterials; Carbadox, Thiamphenicol, Furazolidone, Oxolinic Acid, Sulfadimethoxine, Sulfaquinoxaline, Nalidixic Acid, Piromidic Acid
Pharmaceutical: Antacids ; Famotidine, Cimetidine, Ranitidine
Pharmaceutical: Veterinary drug coccidiostats; 4-amino-3,5-dinitrobenzamide, Zoalene (3,5-nitro-o-toluamide, Nitromid (3,5-dinitrobenzamide), Ethopabate
Pharmaceutical: Antidepressants; Uracil, Acetaminophen, p-Hydroxybenzoic acid, o-Hydroxybenzoic acid, Oxazepam, Diazepam, Di-isopropyl phthalate, Di-npropyl phthalate
11-Hypersil GOLD AX
Biotech: Monophosphates
Food safety: Vitamin C
12-Hypersil GOLD SAX
Monophosphates; Uracil, Cytidine-5’-monophosphate, Adenosine-5’-monophosphate, Guanosine-5’-monophosphate
13- Hypersil GOLD Silica
Forensics: Steroids ; Progesterone, 21-Hydroxyprogesterone-21-acetate, 17-a-Hydroxyprogesterone, Cortisone, 11-a-Hydroxyprogesterone, Corticosterone,
Hydrocortisone
14- Hypersil GOLD HILIC
Food Safety: Water soluble vitamins; Thiamine, Nicotinic acid, Nicotinamide, Pyridoxine, Riboflavin, PABA.
Clinic: Urea
Kaynak;
https://tools.thermofisher.com/content/sfs/brochures/BRCCSGOLDTECHV4_0910_final290910.pdf
http://www.dionex.com/en-us/webdocs/4139-DS_Acclaim_120_LPN1413-02.pdf
https://www.thermofisher.com/tr/en/home/applications-techniques.html
http://www.dionex.com/en-us/documents/application-notes-updates/lp-87103.html
http://www.jascoinc.com/chromatography/uhplc
http://www.sielc.com/Applications_By_Compound.html
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