Download Powerpoint - Circulation Research

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

page
1
page
2
page
3
page
4
page
5
page
6
page
7
page
8
page
9
page
10
Document related concepts
no text concepts found
Transcript 
Inhibition of Vascular Smooth Muscle Cell Growth Through
Antisense Transcription of a Rat Insulin-Like Growth
Factor I Receptor cDNA
by Jie Du, and Patrick Delafontaine
Circulation Research
Volume 76(6):963-972
June 1, 1995
Copyright © American Heart Association, Inc. All rights reserved.
Cloning of rat insulin-like growth factor I receptor (IGF IR) cDNAs.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Construction of episomal vectors pAnti–IGF IR and pAnti–IGF IRi. The rat insulin-like growth
factor receptor I (IGF IR) cDNA clone p118 was digested by Xho I and Kpn I, and the 0.8-kb
restriction fragment was ligated in an antisense orientation into pCEP4 (shown), yielding pAnti–
IGF IR, or into pMEP4, yielding pAnti–IGF IRi. CMV indicates cytomegalovirus immediate-early
gene enhancer/promoter; SVpA, simian virus 40 poly(A); EBNA-1, Epstein-Barr virus (EBV)–
encoded nuclear antigen 1; OriP, EBV origin of replication; Hyg, hygromycin resistance gene;
Amp, ampicillin resistance gene; Km, kanamycin resistance gene; and IGFIR, IGF IR. pAnti–IGF
IRi was constructed in a similar fashion by using pMEP4 (identical to pCEP4 but containing the
human metallothionein IIa promoter).
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Rat insulin-like growth factor I receptor (IGF IR) precursor nucleotide sequence and predicted
amino acid sequence.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Effect of antisense (AS) insulin-like growth factor receptor I (IGF IR) cDNA transcription on IGF
IR mRNA levels.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Effect of inducible antisense insulin-like growth factor receptor I (IGF IR) cDNA transcription on
IGF IR mRNA levels.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Bar graphs showing the effect of antisense insulin-like growth factor receptor I (IGF IR) cDNA
transcription on IGF IR number and on DNA synthesis.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Bar graphs showing the effect of inducible antisense insulin-like growth factor (IGF) I receptor
(IGF IR) cDNA transcription on IGF IR number and on DNA synthesis.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Bar graph showing the effect of antisense insulin-like growth factor (IGF) receptor I (IGF IR)
cDNA transcription on IGF I–induced DNA synthesis.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Effect of antisense insulin-like growth factor receptor I (IGF IR) cDNA transcription on vascular
smooth muscle cell proliferation.
Jie Du, and Patrick Delafontaine Circ Res. 1995;76:963-972
Copyright © American Heart Association, Inc. All rights reserved.
Related documents