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Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 Dr. Yunjun Zhao Postdoc Energy Biosciences Institute University of California, Berkeley 2151 Berkeley Way, Room 220, EBB Berkeley, CA 94720, USA Tel: +1(510) 387-7581 Email: [email protected] or [email protected] Educational Qualifications Ph.D. (2008-2013) Botany, University of Chinese Academy of Sciences/ Institute of Plant Physiology & Ecology, SIBS, CAS, Shanghai, CHINA Dissertation: “Molecular dissection of the PtrMAN6 and PtrWND1B regulation on secondary cell wall formation in Populus trichocarpa”. M.S. (2005-2008) Horticulture, College of Horticulture & forestry Sciences, Huazhong Agricultural University, Wuhan, CHINA Thesis : “Cloning and functional analysis of the promoter of PtCBF1, A CBF1-Like gene from Poncirus trifoliata(L.) Raf” B.S. (2001-2005) Biological sciences, College of Life Sciences, Wuhan University, Wuhan, CHINA Thesis : “Studies on the dynamic trend of diosgenin content in vegetative organs of Dioscorea zingiberensis” Professional Experience 2013.12- present Postdoc Research Supervisor: Associate Professor Markus Pauly 1 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 Projects: 1. Mechanism of O-acetylation of hemicelluloses 2. Genome-wide association studies (GWAS) in lignocellulosic/cell wall research of Sorghum 2008.9- 2013.7 Ph.D. Graduate Research Supervisor: Professor Laigeng Li Projects: 3. Characterization of a vessel specific mannan endo-hydrolase gene (PtrMAN6) during secondary growth in Populus. 4. Glactoglucomannan oligosaccharides (GGMOs) signals in the process of wood formation. 5. Post-translational regulation of PtrMAN6 protein. 6. Functional analysis of the PtrWND1B transcriptional factor which undergoes a wood specific alternative splicing during secondary cell wall thickening in Populus. 2005.9- 2008.6 M.S. Graduate Research Supervisor: Professor Zhonghai Sun Projects: 1. Functional characterization of a cold induced PtCBF1 promoter in Poncirus trifoliata. 2. Transformation of Poncirus trifoliata. 2004- 2005 Undergraduate Research Supervisor: Associate Professor Jiaru Li Projects: 1. Studies on the dynamic trend of diosgenin content in vegetative organs of Dioscorea zingiberensis. 2. ELISA analysis of diosgenin content. Research papers 2 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 1. Yunjun Zhao, Peng Xu, Jiayan Sun, Rui Zhang, and Laigeng Li. (2014) Intron-mediated alternative splicing of WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B regulates cell wall thickening during fiber development in Populus species. Plant Physiol 164(2):765-76 2. Yi Wang, Roger Thilmony, Yunjun Zhao, Guoping Chen, and Yong Qiang Gu. (2014) AIM: a comprehensive Arabidopsis interactome module database and related interologs in plants. Database. DOI: 10.1093/database/bau117 3. Yunjun Zhao, Qian Zhang, Luxia Yuan, Rui Zhang, and Laigeng Li. (2013) N-glycosylation and dimerization regulate the PtrMAN6 enzyme activity that may modulate generation of oligosaccharide signals. Plant Signal Behav 8:e26956 4. Yunjun Zhao, and Laigeng Li. (2013). Plant Endo-β-mannanase Activity Assay. Bio-protocol 3(17): e883. http://www.bio-protocol.org/e883 5. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. (2013) Populus endo-beta-mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with suppression of secondary wall thickening through generation of oligosaccharide signals. Plant J 74(3):473-485. 6. Yunjun Zhao, and Laigeng Li. (2011) Plant cell wall loosening factors. Plant Physiol J 47(10):925-935.(In Chinese, review). 7. Yunjun Zhao, Ligang He, Huiliang Wang, Miao Xu, Dechun Liu, and Zhonghai Sun*. (2007) Cloning and bioinformatics analysis of the promoter of PtCBF1, a CBF-like gene in the citrus relative, Poncirus trifoliata(L.) Raf. J Hunan Agricultural University (Natural Science) 33:45-50. (In Chinese) Abstracts/Poster presentations 1. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. Endo-beta-mannanase mediates an oligosaccharide signaling pathway to regulate cell wall thickening during xylem cell differentiation in Populus. The 6th Youth Symposium on Plant Biology, Shanghai Society for Plant Physiology (SSPP), Chenshan, Shanghai, China, September, 2012. (Invited speaker) 3 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 2. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. PtMAN6, an endo-beta-mannanase gene, mediates an oligosaccharide signaling pathway to regulate cell wall thickening during xylem cell development in poplar. 2012 Annual Conference of Institute of Plant Physiology & Ecology, Qiandaohu, Zhejiang, China, May, 2012. 3. Yunjun Zhao, Dongliang Song, Junhui Shen and Laigeng Li. Functional characterization of mannan endo-1,4-beta-hydrolase in poplar secondary growth. The 10th Conference of Chinese Society of Plant Biology, Kaifeng, Henan, China, August, 2009. (In Chinese) Honors and Awards 2013 Graduate with Distinction at Graduate University of Chinese Academy of Sciences 2012 Excellent Award (first place) at the 6th Youth Symposium on Plant Biology, Shanghai Society for Plant Physiology (SSPP). 2012 Excellent Award (first place) at 2012 Annual Conference of Institute of Plant Physiology & Ecology. 2011 Honor roll student at Graduate University of Chinese Academy of Sciences. Technical Skills 1. Molecular Biology (Nucleic acid extraction, PCR, RT-PCR, Quantitaive Real Time-PCR, Cloning, Transformation, Site-directed mutagenesis, PCR-mediated recombination et.al.) 2. Proteomics (Heterologous protein expression in Escherichia coli, Pichia and tobacco leaf cell, Protein extraction, SDS-PAGE, Western blot, Protein purification and characterization, Enzyme activity assays et.al.) 3. Cell Biology ( Tissue embedding and sectioning, Immunohistochemistry, Confocal microscopy, Transmission electron microscopy, Subcellular protein localization) 4. Cell Wall Biology (Monosaccharide composition via GC-MS and Dionex, Wall-bound acetic acid assay, MLG assay, Acetyl bromide soluble lignin assay, MALDI-TOF, 4 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 Oligosaccharides analysis via Dionex, saccharification assay et.al) 5. Plant culture, transformation, physiology and analysis (Arabidopsis thaliana, Populus ) 6. Computer/Programming skills (Operating Systems: Windows 98/NT/2000/XP, Mac OS; MS office: Word, Excel and Power Point; Bioinformatics: BLAST, ClastW, MEGA, Vector NTI, Bioedit, Primer Premier and various prediction programs; Biostatistics: did courses during my M.S. and B.S.; Others: Adobe Photoshop, EndNote Program, C Language: national computer rank examination certificate grade 2) Teaching and Supervision Experience 1. Rotation and Ph.D. students 2012, Qian Zhang, Institute of Plant Physiology and Ecology, SIBS, CAS, Project: Heterologous expression and characterization of several mutagenized mannan endo-hydrolases. 2009, Hua Zhang, Institute of Plant Physiology and Ecology, SIBS, CAS, Project: Cloning of several genes involved in plant secondary growth. 2. Visiting M.S. Student 2014, Ronald Colin Jaepel, RWTH Aachen University, Germany Project: Genetic regulation of cell wall acetylation. 2011-2012, Ting Huang, Central South University of Forestry and Technology, China Project: Cloning and characterization of two putative fiber-specific promoters in Populus. 3. Undergraduate students 2014, Brett Michael Garabedian, University of California, Berkeley, USA Project: Protocol development for the quantification of Glucomannan-bound acetate in Arabidopsis. 2013, Shuai Zheng, Henan University, China Project: Characterization of the alternative splicing mechanism for a NAC transcriptional factor in Populus. 2011, Shujie Wang, Soochow University, China 5 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 Project: Cloning and characterization of a putative fiber-specific promoter in Oryza sativa. 2010, Lina Yu, University of Shanghai, China Project: Cloning and transformation of a gene invoved in secondary cell wall formation. Services to the scientific community Reviewer for Planta Key collaborators 1. Markus Pauly - Department of Plant and Microbial Biology, Energy Biosciences Institute, University of California, Berkeley, CA, 94720, Biosynthesis, structure and function of Plant cell wall 2. Laigeng Li - Institute of Plant Physiology & Ecology, SIBS, CAS, China, Plant cell wall biosynthesis and secondary growth 3. Yihua Zhou - Institute of Genetics and Developmental Biology, CAS, China, Cell wall compositions analysis Personal Details Date of Birth: July 25 Gender: Male Place of Birth: Nanjing, Jiangsu, China Citizenship: Chinese Languages: Chinese, English Referees 1. Prof. Markus Pauly Department of Plant and Microbial Biology, Energy Biosciences Institute, University of 6 Yunjun’s Curriculum Vitae Updated on Jan 9th, 2015 California, Berkeley 2151 Berkeley way, Berkeley, CA, 94720 Tel. +1-510-642-1722 (phone) +1-510-642-1490 (fax) Email: [email protected] (e-mail) Web: http://epmb.berkeley.edu/facPage/dispFP.php?I=1635 2. Prof. Laigeng Li Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science 300 Fenglin Rd, Shanghai, P.R. China, 200032 Tel. +86-21-54924161 (lab) Email: [email protected] Web: http://sippe.ac.cn/llg/ 3. Prof. Chi-Kuang Wen Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science 300 Fenglin Rd, Shanghai, P.R. China, 200032 Tel: +86-21-54924060 (lab). Email: [email protected] Web: http://sourcedb.cas.cn/sourcedb_sibs_cas/yw/rck/200906/t20090629_1856153.html 7