Download Yunjun`s CV - Pauly Lab - University of California, Berkeley

Yunjun’s Curriculum Vitae
Updated on Jan 9th, 2015
Dr. Yunjun Zhao
Postdoc
Energy Biosciences Institute
University of California, Berkeley
2151 Berkeley Way, Room 220, EBB
Berkeley, CA 94720, USA
Tel: +1(510) 387-7581
Email: [email protected] or [email protected]
Educational Qualifications
Ph.D. (2008-2013)
Botany, University of Chinese Academy of Sciences/ Institute of Plant
Physiology & Ecology, SIBS, CAS, Shanghai, CHINA
Dissertation: “Molecular dissection of the PtrMAN6 and PtrWND1B
regulation on secondary cell wall formation in Populus trichocarpa”.
M.S. (2005-2008)
Horticulture, College of Horticulture & forestry Sciences,
Huazhong Agricultural University, Wuhan, CHINA
Thesis : “Cloning and functional analysis of the promoter of PtCBF1, A
CBF1-Like gene from Poncirus trifoliata(L.) Raf”
B.S. (2001-2005)
Biological sciences, College of Life Sciences,
Wuhan University, Wuhan, CHINA
Thesis : “Studies on the dynamic trend of diosgenin content in vegetative
organs of Dioscorea zingiberensis”
Professional Experience
2013.12- present
Postdoc Research
Supervisor: Associate Professor Markus Pauly
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Projects:
1. Mechanism of O-acetylation of hemicelluloses
2. Genome-wide association studies (GWAS) in lignocellulosic/cell wall research of
Sorghum
2008.9- 2013.7
Ph.D. Graduate Research
Supervisor: Professor Laigeng Li
Projects:
3. Characterization of a vessel specific mannan endo-hydrolase gene (PtrMAN6) during
secondary growth in Populus.
4. Glactoglucomannan oligosaccharides (GGMOs) signals in the process of wood formation.
5. Post-translational regulation of PtrMAN6 protein.
6. Functional analysis of the PtrWND1B transcriptional factor which undergoes a wood
specific alternative splicing during secondary cell wall thickening in Populus.
2005.9- 2008.6
M.S. Graduate Research
Supervisor: Professor Zhonghai Sun
Projects:
1. Functional characterization of a cold induced PtCBF1 promoter in Poncirus trifoliata.
2. Transformation of Poncirus trifoliata.
2004- 2005
Undergraduate Research
Supervisor: Associate Professor Jiaru Li
Projects:
1. Studies on the dynamic trend of diosgenin content in vegetative organs of Dioscorea
zingiberensis.
2. ELISA analysis of diosgenin content.
Research papers
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Updated on Jan 9th, 2015
1. Yunjun Zhao, Peng Xu, Jiayan Sun, Rui Zhang, and Laigeng Li. (2014) Intron-mediated
alternative splicing of WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B
regulates cell wall thickening during fiber development in Populus species. Plant Physiol
164(2):765-76
2. Yi Wang, Roger Thilmony, Yunjun Zhao, Guoping Chen, and Yong Qiang Gu. (2014)
AIM: a comprehensive Arabidopsis interactome module database and related interologs in
plants. Database. DOI: 10.1093/database/bau117
3. Yunjun Zhao, Qian Zhang, Luxia Yuan, Rui Zhang, and Laigeng Li. (2013)
N-glycosylation and dimerization regulate the PtrMAN6 enzyme activity that may
modulate generation of oligosaccharide signals. Plant Signal Behav 8:e26956
4. Yunjun Zhao, and Laigeng Li. (2013). Plant Endo-β-mannanase Activity Assay.
Bio-protocol 3(17): e883. http://www.bio-protocol.org/e883
5. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. (2013) Populus
endo-beta-mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with
suppression of secondary wall thickening through generation of oligosaccharide signals.
Plant J 74(3):473-485.
6. Yunjun Zhao, and Laigeng Li. (2011) Plant cell wall loosening factors. Plant Physiol J
47(10):925-935.(In Chinese, review).
7. Yunjun Zhao, Ligang He, Huiliang Wang, Miao Xu, Dechun Liu, and Zhonghai Sun*.
(2007) Cloning and bioinformatics analysis of the promoter of PtCBF1, a CBF-like gene
in the citrus relative, Poncirus trifoliata(L.) Raf. J Hunan Agricultural University
(Natural Science) 33:45-50. (In Chinese)
Abstracts/Poster presentations
1. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. Endo-beta-mannanase
mediates an oligosaccharide signaling pathway to regulate cell wall thickening during
xylem cell differentiation in Populus. The 6th Youth Symposium on Plant Biology,
Shanghai Society for Plant Physiology (SSPP), Chenshan, Shanghai, China, September,
2012. (Invited speaker)
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2. Yunjun Zhao, Dongliang Song, Jiayan Sun, and Laigeng Li. PtMAN6, an
endo-beta-mannanase gene, mediates an oligosaccharide signaling pathway to regulate
cell wall thickening during xylem cell development in poplar. 2012 Annual Conference
of Institute of Plant Physiology & Ecology, Qiandaohu, Zhejiang, China, May, 2012.
3. Yunjun Zhao, Dongliang Song, Junhui Shen and Laigeng Li. Functional characterization
of mannan endo-1,4-beta-hydrolase in poplar secondary growth. The 10th Conference of
Chinese Society of Plant Biology, Kaifeng, Henan, China, August, 2009. (In Chinese)
Honors and Awards
2013
Graduate with Distinction at Graduate University of Chinese Academy of
Sciences
2012
Excellent Award (first place) at the 6th Youth Symposium on Plant Biology,
Shanghai Society for Plant Physiology (SSPP).
2012
Excellent Award (first place) at 2012 Annual Conference of Institute of Plant
Physiology & Ecology.
2011
Honor roll student at Graduate University of Chinese Academy of Sciences.
Technical Skills
1. Molecular Biology (Nucleic acid extraction, PCR, RT-PCR, Quantitaive Real Time-PCR,
Cloning, Transformation, Site-directed mutagenesis, PCR-mediated recombination et.al.)
2. Proteomics (Heterologous protein expression in Escherichia coli, Pichia and tobacco leaf
cell, Protein extraction, SDS-PAGE, Western blot, Protein purification and
characterization, Enzyme activity assays et.al.)
3. Cell Biology ( Tissue embedding and sectioning, Immunohistochemistry, Confocal
microscopy, Transmission electron microscopy, Subcellular protein localization)
4. Cell Wall Biology (Monosaccharide composition via GC-MS and Dionex, Wall-bound
acetic acid assay, MLG assay, Acetyl bromide soluble lignin assay, MALDI-TOF,
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Oligosaccharides analysis via Dionex, saccharification assay et.al)
5. Plant culture, transformation, physiology and analysis (Arabidopsis thaliana,
Populus )
6. Computer/Programming skills (Operating Systems: Windows 98/NT/2000/XP, Mac
OS; MS office: Word, Excel and Power Point; Bioinformatics: BLAST, ClastW, MEGA,
Vector NTI, Bioedit, Primer Premier and various prediction programs; Biostatistics: did
courses during my M.S. and B.S.; Others: Adobe Photoshop, EndNote Program, C
Language: national computer rank examination certificate grade 2)
Teaching and Supervision Experience
1. Rotation and Ph.D. students
2012, Qian Zhang, Institute of Plant Physiology and Ecology, SIBS, CAS,
Project: Heterologous expression and characterization of several mutagenized mannan
endo-hydrolases.
2009, Hua Zhang, Institute of Plant Physiology and Ecology, SIBS, CAS,
Project: Cloning of several genes involved in plant secondary growth.
2. Visiting M.S. Student
2014, Ronald Colin Jaepel, RWTH Aachen University, Germany
Project: Genetic regulation of cell wall acetylation.
2011-2012, Ting Huang, Central South University of Forestry and Technology, China
Project: Cloning and characterization of two putative fiber-specific promoters in Populus.
3. Undergraduate students
2014, Brett Michael Garabedian, University of California, Berkeley, USA
Project: Protocol development for the quantification of Glucomannan-bound acetate in
Arabidopsis.
2013, Shuai Zheng, Henan University, China
Project: Characterization of the alternative splicing mechanism for a NAC transcriptional
factor in Populus.
2011, Shujie Wang, Soochow University, China
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Project: Cloning and characterization of a putative fiber-specific promoter in Oryza
sativa.
2010, Lina Yu, University of Shanghai, China
Project: Cloning and transformation of a gene invoved in secondary cell wall formation.
Services to the scientific community
Reviewer for Planta
Key collaborators
1. Markus Pauly - Department of Plant and Microbial Biology, Energy Biosciences Institute,
University of California, Berkeley, CA, 94720, Biosynthesis, structure and function of
Plant cell wall
2. Laigeng Li - Institute of Plant Physiology & Ecology, SIBS, CAS, China, Plant cell wall
biosynthesis and secondary growth
3. Yihua Zhou - Institute of Genetics and Developmental Biology, CAS, China, Cell wall
compositions analysis
Personal Details
Date of Birth: July 25
Gender: Male
Place of Birth: Nanjing, Jiangsu, China
Citizenship: Chinese
Languages: Chinese, English
Referees
1. Prof. Markus Pauly
Department of Plant and Microbial Biology, Energy Biosciences Institute, University of
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California, Berkeley
2151 Berkeley way, Berkeley, CA, 94720
Tel. +1-510-642-1722 (phone) +1-510-642-1490 (fax)
Email: [email protected] (e-mail)
Web: http://epmb.berkeley.edu/facPage/dispFP.php?I=1635
2. Prof. Laigeng Li
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Science
300 Fenglin Rd, Shanghai, P.R. China, 200032
Tel. +86-21-54924161 (lab)
Email: [email protected]
Web: http://sippe.ac.cn/llg/
3. Prof. Chi-Kuang Wen
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Science
300 Fenglin Rd, Shanghai, P.R. China, 200032
Tel: +86-21-54924060 (lab).
Email: [email protected]
Web: http://sourcedb.cas.cn/sourcedb_sibs_cas/yw/rck/200906/t20090629_1856153.html
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