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Identifying the Properties of Respiration Bell Work: 3/4/14 • Please pick up your binder and a copy of today’s notes, “Identifying the properties of Respiration.” Photosynthesis - Respiration Comparison • Photosynthesis • Respiration • Occurs only in green plants • Occurs only in green plant cells • Proceeds only in light • Food is made • Increases dry weight • CO2, H2O are used • Produces sugars and O2 • Stores energy in sugar • ATP & NADPH produced • ATP & NADPH used for synthesis of sugar • Occurs in all plants and animals • Occurs in all living cells • Proceeds in light and darkness • Food is used • Decreases dry weight • Sugar and O2 are used • Produces CO2 and H2O • Releases energy • ATP produced • ATP used for many reactions Photosynthesis Light Reaction Hill Reaction (Granum) Dark Reaction Calvin Cycle (Stroma) Respiration Plant Respiration Plants breath and need to make energy! Respiration • Occurs in both light and darkness Respiration has Three Processes • 1. Glycolysis • 2. Krebs Cycle • 3. Electron Transport Chain Respiration has Three Processes Respiration • Respiration is the process by which cells of a living organism use energy stored in food molecules to make ATP. • The bonds between the phosphate groups of ATP are unstable and their hydrolysis (where the bonds are broken) releases energy that can be used to drive other cellular processes. Respiration - Definition • Chemical process by which sugars and starches produced by photosynthesis are converted to energy Respiration • Carbohydrates made during Photosynthesis are of value to the plant when they are converted to energy. • This energy is used in the process of building new tissues, or plant growth. • The chemical process by which sugars and starches produced in Photosynthesis are converted to energy is called RESPIRATION. Respiration • In animals, blood carries CO2 and O2 to and from atmosphere by lungs and gills. • In plants, these gases diffuse into plants through the stomata. Respiration • Occurs in all life forms and in the mitochondria organelle of all cells. Mitochondria • The Mitochondria contain the Cristae & the Matrix. • Cristae: Highly folded inner membrane • Matrix: Space inside the inner membrane. Dividing Mitochondria Mitochondria Cristae Matrix A Respiration Flow Chart Respiration - Equation Glucose + Water + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6 H2O + 6 O2 6CO2 + 12 H2O + e Respiration General Reaction • The equation tells us that glucose reacts with oxygen to produce carbon dioxide and water with the release of energy. • Energy can be electrons or ATP. ATP is formed in Glycolysis, the Kreb Cycle and the Electron Transport Chain. Respiration • The raw materials used in Respiration flow into the mitochondria, and from them useable energy emerges. Respiration • Plants use Oxygen in Respiration during the day as well as the night. Carbon Dioxide is one of the end products of Respiration in plants, just as in animals. Photosynthesis - Equation General Reaction Green Plant Glucose + Oxygen + Light EnergyWater Carbon Dioxide + Water Green Plant 6 CO2 + 12 H2O Light Energy C6H12O6 + 6 H2O + 6 O2 Respiration • Consists of three major processes: • 1. • 2. • 3. Glycolysis Krebs Cycle Electron Transport Chain • 1. Respiration Glycolysis • Takes place in the cytoplasm of the cell and requires no oxygen. Glycolysis • In Glycolysis, a 6-carbon sugar is broken down into two, 3-carbon molecules called pyruvate. In the process, ATP is formed, and electrons are delivered to the ETC. • 1. Glycolysis Respiration • Occurs in the cytoplasm • Glucose is broken down to form Pyruvate into Pyruvic Acid. • Two ATP’s are made. • Electrons are delivered to the ETC. Glycolysis • In Glycolysis, glucose is broken down into two-3carbon molecules called Pyruvate. In the process, two ATP’s are used up and four ATP’s are made. Glycolysis • This results in a net yield of two ATP’s. • Four Electrons are delivered to the ETC. Essence of Glycolysis • In a series of reactions, the glucose molecule becomes a fructose molecule, carrying two phosphates. (P) • This fructose molecule is split into 3-carbon fragments. NADH2 & ATP is released. • Some Hydrogen, energy, and water are removed from these 3-carbon fragments forming Pyruvate, which degrades to Pyruvic Acid. Glycolysis - Review • Occurs in the Cytoplasm of the cell. • Glucose is broken down to form Pyruvate. • Two molecules of NADH are made. • Two ATP’s are made. • Electrons are delivered to the ETC. Glycolysis - Produces Energy Glucose + Water + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6 H2O + 6 O2 6CO2 + 12 H2O + e Respiration • Consists of three major processes: • 1. • 2. • 3. Glycolysis Krebs Cycle Electron Transport Chain Sir Hans A. Krebs Sir Hans A. Krebs • The Krebs Cycle was named after Hans Krebs, a British biochemist. • Born in Germany in 1900 • Professor of Biochemistry, Oxford, 1945 • Discovered the citric acid cycle (Krebs Cycle) of respiration in 1953. • Won the Nobel Laureate in Medicine, 1953 • Knighted in 1958 • Died 1981 Krebs Cycle • Before entering the Krebs Cycle, which takes place in the fluid Matrix within the compartments formed by the Cristae of the Mitochondria, Carbon Dioxide is released from the Pyruvate produced by Glycolysis. Krebs Cycle • During the Krebs Cycle, three molecules of NADH and one molecule of FADH2 (electron carriers) are formed. • One molecule of ATP is generated. Krebs Cycle • NADH and FADH2 (electron carriers) go on to deliver their electrons to the electron transport chain. • 2. Respiration Krebs cycle • Takes place in the matrix of the mitochondria. • The Cycle releases Carbon Dioxide. • NADH & FADH2 deliver their electrons to the ETC. • ATP is generated. Respiration - Krebs Cycle Respiration - Krebs Cycle Krebs Produces CO2 & Energy Glucose + Water + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6 H2O + 6 O2 6CO2 + 12 H2O + e Respiration - Krebs Cycle Review • Takes place in the matrix of the mitochondria • The Cycle releases Carbon Dioxide. • NADH & FADH2 deliver their electrons to the ETC. • ATP is generated Respiration • Consists of three major processes: • 1. • 2. • 3. Glycolysis Krebs Cycle Electron Transport Chain • 3. Respiration Electron transport chain - ETC • The ETC is located in the Inner Mitochondrial Membrane. • The ETC moves electrons down the chain producing ATP from ADP. • Uses Oxygen and Hydrogen to release water. Electron Transport Chain Produces Water & Energy Glucose + Water + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6 H2O + 6 O2 6CO2 + 12 H2O + e Respiration - Electron Transport Chain Electron Transport Chain • Electrons from the Krebs Cycle electron carriers NADH & FADH2 are delivered to the Electron Transport Chain. Electron Transport Chain - ETC • The Electron Transport Chain is located in the inner mitochondrial membrane. The “chain” uses the energy of the “delivered” electrons to pump protons (H+) from the matrix of the mitochondria to the inner membrane space. Electron Transport Chain - ETC • This creates a proton gradient across the membrane (more protons in the inter-membrane space than the matrix). These protons move back across the membrane (down the gradient) through the protein complex called ATP synthase. Electron Transport Chain • This “movement” of protons through the ATP synthase, powers the synthesis of ATP from ADP. Notes on The ETC • 1. The ETC is located in the Inner Mitochondrial Membrane. • 2. The ETC moves electrons down the chain producing ATP from ADP. • 3. Releases water Respiration Summary of Respiration • Respiration consists of Three Major Processes: • GYLCOLYSIS • KREBS CYCLE • ELECTRON TRANSPORT CHAIN Factors that Influence Respiration • 1. Temperature • 2. Oxygen Concentration • 2. Soil Conditions • 4. Light Factors Influencing Rates of Respiration • 1. Temperature • There is a positive relationship between temperature and respiration. This is usually within the boundaries of 32oF and 95oF. • Increased temperature means increased respiration. • Refrigeration of stored plant parts (tomatoes, apples, bananas, and flowers) reduces respiration rates Factors Influencing Rates of Respiration • 2. Oxygen concentration • Concentrations of O2 below 21% result in a decrease in respiration. • Increasing O2 concentrations above 21% does not result in increased respiration rates. Factors Influencing Rates of Respiration • 3. Soil Conditions • Compacted or water-logged soils reduces aeration. • Results in reduced root respiration and poor plant growth • 4. Factors Influencing Rates of Respiration Light • Light Intensity and duration affect respiration. • Plants grown in low light exhibit reduced respiration rates. • Low light reduces photosynthesis. • Decreases the amount of carbohydrates produced and available for respiration. Factors that Influence Respiration • 1. Temperature • 2. Oxygen Concentration • 2. Soil Conditions • 4. Light Respiration & Plant Growth • Plant Growth depends on products like carbohydrates that result from photosynthesis and also from mitochondrial respiration for energy and tissue building compounds. Respiration & Plant Growth • Under favorable conditions the corn plant synthesizes (Photosynthesis) about 9 grams of sugar per day. While the average amount respired is two grams per day. The average net gain is 7 grams. Respiration & Plant Growth • Therefore the amount made is greater than the amount used and there is a surplus for growth and storage. When the amount made equals the amount used, there is no surplus for growth. Respiration Review Photosynthesis - Respiration • Respiration • Occurs in all plants and animals • Occurs in all living cells • Proceeds in light and darkness • Food is used • Decreases dry weight • Sugar and O2 are used • Produces CO2 and H2O • Releases energy • ATP produced • ATP is used for many reactions Respiration • Occurs in both light and darkness Respiration - Definition • Chemical process by which sugars and starches produced by photosynthesis are converted to energy Respiration • Occurs in all life forms and in the mitochondria organelle of all cells Respiration • Consists of three major processes: • 1. • 2. • 3. Glycolysis Krebs Cycle Electron Transport Chain Respiration - Glycolysis Review • Occurs in the Cytoplasm of the Cell. • Glucose is broken down to form Pyruvate. • Two molecules of NADH are made. • Two ATP’s are made. • Electrons are delivered to the ETC. Respiration - Krebs Cycle Review • Takes place in the matrix of the mitochondria • The Cycle releases Carbon Dioxide. • NADH & FADH2 deliver their electrons to the ETC. • ATP is generated Respiration - ETC Review • The ETC is located in the Inner Mitochondrial Membrane. • The ETC moves electrons down the chain producing ATP from ADP. • Releases water Respiration Factors that Influence Respiration • 1. Temperature • 2. Oxygen Concentration • 2. Soil Conditions • 4. Light Who is this Guy?