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BIOGRAPHICAL SKETCH
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NAME
POSITION TITLE
Mudgett, Mary Beth
Associate Professor of Biology
Stanford University
eRA COMMONS USER NAME (credential, e.g., agency login)
MUDGETTMARY_BETH
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and
residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
Ithaca College, Ithaca, NY
University of California, Los Angeles, CA
University of California, Los Angeles, CA
University of California, Berkeley, CA
B.A.
Ph.D.
Postdoc
Postdoc
05/89
06/94
12/95
12/01
Biochemistry
Biochemistry
Biochemistry
Plant-Microbe Inter.
A. Personal Statement
My laboratory studies the biochemical mechanisms used by bacterial pathogens to alter plant
physiology during infection. The long-term goal of our work is thus to elucidate the biochemical activities of
bacterial virulence factors secreted by the type III secretory (T3S) apparatus, identify their host targets, and
provide fundamental knowledge of how perturbation of these distinct nodes in plant signal transduction
pathways leads to bacterial pathogenesis. To do so, we study bacterial leaf spot disease in tomato caused by
the Gram-negative, facultative parasite Xanthomonas campestris pathovar vesicatoria (Xcv).
We performed a novel genetic screen to identify new Xcv T3S effector proteins translocated directly
into host plant cells during infection. We biochemically characterized the function of three Xcv T3S effectors –
XopN, XopD, and AvrBsT. We have discovered that these effectors target three distinct nodes in defense
signal transduction. We have shown that: 1) XopN encodes a novel scaffolding-like protein that suppresses
PAMP-triggered immunity and physically interacts with a tomato receptor kinase and the 14-3-3 protein TFT1.
2) XopD encodes a plant-specific SUMO protease that alters host transcription, promotes pathogen growth,
and suppresses host defense responses at the late stages of tissue colonization. 3) AvrBsT interferes with lipid
signaling, resulting in the activation of effector-triggered immunity.
Ongoing work is aimed at elucidating how the identified host targets regulate plant immunity during
pathogen attack, as well as the biochemical characterization of novel Xcv effector activities. Understanding
how plant immunity is regulated and how bacterial pathogens manipulate their hosts is fundamental knowledge
required for the prevention and elimination of plant disease.
B. Positions and Honors
Positions
2002-2009
2010-
Assistant Professor of Biology, Stanford University, Stanford, CA
Associate Professor of Biology, Stanford University, Stanford, CA
Other Experience and Professional Memberships
1996 –
Member of International Society of Plant-Microbe Interactions
1996 –
American Society for Plant Biology
2005 –
NIH HIBP Study Section, Ad hoc
2008 –
NSF IOS Symbiosis, Defense and Self-recognition, Ad hoc and Grant Review Panel
2009 – 2011 Senior Editor, Molecular Plant Pathology
2009 –
Research Advisory Board, Pierce’s Disease/Glassy Wing Sharp Shooter Program
2010 –
College Board AP Biology Curriculum Review and Research
2010 –
Editor, Frontiers in Plant-Microbe Interactions
2012
Co-organizer, BARD Workshop: Microbial Virulence Determinants & Plant Immunity, Israel
2012 –
Chair, Pierce’s Disease/Glassy Wing Sharp Shooter Research Advisory Board
2012 –
Board Member, International Society for Molecular Plant-Microbe Interactions
Honors
1990
1992
1997-1999
2004-2010
2005-2006
2008
2011-2014
2013-2014
Graduate Teaching Assistant Award, UCLA
Graduate Research Achievement Award, UCLA
National Research Service Award, NIH
Terman Fellow, Stanford University
Hellman Faculty Scholar, Stanford University
Teacher of the Year, Associated Students of Stanford University
Chamber Fellowship, Stanford University
VPUE Faculty Scholar, Stanford University
C. Selected Peer-reviewed Publications (selected from 30 peer-reviewed publications).
Most relevant to the current application
1. Cunnac, S., Wilson, A., Nuwer, J., Kirik, A., Baranage, G., and Mudgett, M. B. (2007) The conserved
Arabidopsis carboxylesterase SUPPRESSOR OF AVRBST-ELICITED RESISTANCE inhibits defenses
triggered by the AvrBsT Type III Effector. Plant Cell 19, 688-705. PMID:17293566.
2. Kim, J.-G., Taylor, K. W., Hotson, A., Keegan, M., Schmeltz, E. A., and Mudgett, M. B. (2008) XopD SUMO
protease affects host transcription, promotes pathogen growth, and delays symptom development in
Xanthomonas-infected tomato leaves. Plant Cell 20, 1915-1929. PMID:18664616.
3. Kirik, A. and Mudgett, M. B. (2009) SOBER1 hydrolase suppresses phosphatidic acid accumulation and
plant defense in response to bacterial T3S effector AvrBsT. Proc. Natl. Acad. Scie. U.S.A. 106, 2053220537. PMID:19918071.
4. Taylor, K. W., Kim, J.-G., Su, X. B., Aakre, C. D., Roden, J. A., Adams, C. M., and Mudgett, M.B. (2012)
Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from
binding to TFT1 attenuate Xanthomonas virulence. PLoS Pathogens 8, e1002768. PMID:22719257.
5. Kim, J.-G. Stork, W., and Mudgett, M. B. (2013) Xanthomonas type III effector XopD desumoylates tomato
transcription factor SlERF4 to suppress ethylene responses and promote pathogen growth. Cell Host
Microbe 13, 1-12. PMID:23414755.
Additional recent publications of importance to the field (in chronological order)
1. Orth, K., Xu, Z., Mudgett, M. B., Bao, Z. Q., Palmer, L. E., Bliska, J. B., Mangel, W. F., Staskawicz, B., and
Dixon, J. E. (2000) Disruption of signaling byYersinia effector YopJ, a ubiquitin-like protein protease.
Science 290, 1594-1597. PMID:11090361.
2. Hotson, A., Chosed, R., Shu, H., Orth, K., and Mudgett, M. B. (2003) Xanthomonas type III effector XopD
targets SUMO-conjugated proteins in planta. Mol. Microbiol. 50, 377-389. PMID:14617166.
3. Roden, J. A., Belt, B., Ross, J.B., Tachibana, T., Vargas, J., and Mudgett, M. B. (2004) A genetic screen to
isolate type III effectors translocated into pepper cells during Xanthomonas infection. Proc. Natl. Acad. Sci.
U.S.A. 101, 16624-16629. PMID:15545602.
4. Mudgett, M. B. (2005) New insights to the function of phytopathogenic bacterial type III effectors in plants.
Ann. Rev. Plant Biol. 56, 509-531. PMID:15862106.
5. Doyle, E. A., Lane, A. M., Sides, J. M., Mudgett, M. B., and Monroe, J. D. (2007) An alpha-amylase
(At4g25000) in Arabidopsis leaves is secreted and induced by biotic and abiotic stress. Plant Cell Environ.
30, 388-398. PMID:17324226.
6. Swartz, T.E., Tseung, T.-S., Frederickson, M., Paris, G., Comerci, D. J., Rajashekara, G., Kim, J.-G.,
Mudgett, M.B., Splitter, G., Ugalde, R. A., Goldbaum, F. A., Briggs, W. R., and Bogomolni, R. A. (2007)
Blue-light-activated Histidine kinases: two-component sensors in bacteria. Science 317, 1090-1093.
PMID:17717187.
7. Kim, J.-G., Xinyan, L., Roden, J., Taylor, K., Aakre, C., Su, B., Lalonde, S., Kirik, A., Chen, Y., Baranage,
G., McLane, H., Martin, G. B., and Mudgett, M. B. (2009) Xanthomonas T3S effector XopN suppresses
PAMP-triggered immunity and physically interacts with a tomato atypical receptor-like kinase and TFT1 in
planta. Plant Cell 21, 1305-1323. PMID:19366901.
8. Chen, L-Q., Hou, B.-H., Lalonde, S., Takanaga, H., Hartung, M.L., Qu, X.-Q., Guo, W.-J., Kim, J.-G.,
Underwood, W., Chaudhuri, B., Chermak, D., Antony, G., White, F. F., Somerville, S. C., Mudgett, M. B.,
and Frommer, W. B. 2010) Sugar transporters for intercellular exchange and nutrition of pathogens. Nature
468, 527-532. PMID:21107422.
9. Kim, J.-G., Taylor, K., and Mudgett, M. B. (2011) Comparative analysis of the XopD T3S effector family in
plant pathogenic bacteria. Mol. Plant. Pathol. 12, 715-730. PMID:21726373.
10. Sonnewald, S., Priller, J., Schuster, J. Glickmann, E., Hajirezaei, M.-R., Siebig, St., Mudgett, M. B., and
Sonnewald, U. (2012) Regulation of cell wall-bound invertase in pepper leaves by Xanthomonas
campestris pv. vesicatoria Type Three Effectors. PLoS One 7(12), e51763. PMID:23272161.
C. Research Support
Ongoing Research Support
NIH/NIGMS 2 RO1 GM068886-06A1
Mudgett (PI)
09/30/04 – 04/30/14
Characterization of lipid signaling altered by T3S effector AvrBsT
The goal of this project is to determine how AvrBsT-triggered lipid signals activate immune signal transduction
during bacterial infection in Arabidopsis.
BSF
2011069
Mudgett (co-PI)
10/01/12 – 09/30/16
Tomato 14-3-3 proteins: signaling components of plant immunity and targets of bacterial virulence
determinants
The goal of this project is to determine the Xanthomonas T3S effector suite that interact with tomato 14-3-3
proteins to determine how and why 14-3-3’s are being co-opted during infection to promote pathogenesis.
Completed Research Support
DOE
DE-FG02-03ER15443
Mudgett (PI)
09/01/03 – 08/31/06
Molecular communication between Xanthomonas campestris pv. vesicatoria and its plant hosts: Genome-wide
survey to identify type III effector proteins and to characterize their role in plant pathogenesis.
The goal of this project was to identify type III effectors in Xanthomonas to gain insight to the virulence factors
that control bacterial spot disease in tomato.
DOE
DE-GF-2-03ER15443-A002 Mudgett (PI)
09/01/06 – 08/31/07
Biochemical characterization of the interaction between the Xanthomonas type III effector XopN and a tomato
LRR-RLK.
The goal of this project was to identify plant binding partners for the bacterial protein XopN to begin to
elucidate the nodes in the plant immune system that under attack during infection.
BARD
Mudgett (co-PI)
02/05/12 – 02/09/12
BARD Workshop: Microbial virulence determinants and plant immunity.
The goal of this workshop was to unite Israeli and American scientists working on plant-microbe interactions to
share research technology, establish new collaborations, and plan for future work in the field.
NSF/IOS 1160811
Mudgett (PI)
01/01/12 – 06/30/12
BARD Workshop on microbial virulence determinants and plant innate immunity.
Funding was obtained to support young and established American scientists to attend the workshop in Israel.
Company of Biologists LTD
Mudgett (PI)
01/31/12
Workshop support for European scientists
Funding was obtained to support participants from Europe and Asia to attend the workshop in Israel.
NSF/IOS 821801
Mudgett (PI)
09/01/08 – 08/31/13
Characterization of XopN, a heat-repeat TTSS effector required for Xanthomonas pathogenesis in tomato.
The goal of this project was to determine how the bacterial protein XopN suppresses host immune signaling by
targeting the receptor kinase TARK1 and the 14-3-3 phospho-binding protein TFT1.