Download Acyl carrier protein From E. coli, recombinant Overexpressed in E

Acyl carrier protein
From E. coli, recombinant
Overexpressed in E.coli
Product Number A8725
Storage Conditions –70 °C
Product Description
1
λmax : 276 nm
Extinction Coefficient: EM = 1,800 at 280 nm
1
(10 mM PIPES, pH 6.8)
4
Isoelectric point: 4.1
Acyl carrier protein is recombinant, overexpressed in
Eschericia coli. Acyl carrier protein is as an acidic,
1
asymmetric monomer with a molecular weight of
2,3
8,847 by sequence analysis.
Acyl carrier protein
binds less SDS than typical globular proteins and
exhibits anomalous mobility in SDS electrophoresis
5
systems.
Acyl moieties bound during fatty acid biosynthesis or
glycerolipid synthesis do so as thioesters of the only
sulfhydryl group of the protein, located on the terminal
portion of the 4'-phosphopantetheine prosthetic group
36 6
on Ser .
The product is supplied as a solution in 25 mM MES,
pH 6.1, with 0.1 M NaCl.
References
1. Rock, C. O., and Cronan, J. E. Jr., Improved
purification of acyl carrier protein. Anal. Biochem.,
102, 362-364 (1980).
2. Vanaman, T. C., et al., The complete amino acid
sequence of the acyl carrier protein of Escherichia
coli. J. Biol. Chem., 243, 6420-6431 (1968).
3. Ray, T. K., and Cronan, J. E., Jr., Acylation of snlycerol 3-phosphate in Escherichia coli. Study of
reaction with native palmitoyl-acyl carrier protein.
J. Biol. Chem., 250, 8422-8427 (1975).
4. Rock, C. O., and Cronan, J. E., Jr., Acyl Carrier
Protein from Eschericia coli. Methods in
Enzymology 71, 341-351(1981).
5. Rock, C. O., and Cronan, J. E., Jr., Re-evaluation
of the solution structure of acyl carrier protein. J.
Biol. Chem., 254, 9778-9785 (1979).
6. Rock, C. O., et al., Molecular properties of acyl
carrier protein derivatives. J. Biol. Chem., 256,
2669-2674 (1981).
MES 1/08
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses.
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