Download Chicken Infectious Bronchitis Virus (IBV) ELISA Kit, Cat # 920-120-IBV

Chicken Infectious Bronchitis Virus (IBV) ELISA Kit, Cat # 920-120-IBV
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ELISA Kit Features
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Purified Infectious Bronchitis Virus (IBV) viral antigens-coated,
stabilized, 96-well strip plate
Negative and Positive controls
100ul samples (diluted 1:100 or more)
105 min, 3 incubation step at room temp
Detects antibody titer in the egg yolks of vaccinate or immunized
chickens (no need for blood samples) or in serum/plasma.
Shelf life >6 months or more.
This kit is for measuring anti-IBV Antibody in chicken serum or plasma or egg
yolks. For in vitro research use only.
Assay Procedure: Allow all reagents to reach room temperature. Arrange and label required number of strips.
Step 1. Pipet 100 ul each of negative and positive cotnrols, and diluted samples into wells. Mix gently and incubate at room
temperature for 60 min.
Step 2. Aspirate and wash the plate four times. Add 100ul of Antibody-HRP Conjugate to all wells, mix gently and incubate at
room temperature for 30 min.
Step 3. Aspirate and wash the plate five times. Add 100 ul of TMB Substrate solution to all wells, mix gently, and incubate at
room temperature for 15 min.
Step 4. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A450 nm.
Determine the presence of chicken anti-Influenza A IgG by comparing with the supplied –ve control and positive calibrator.
General Information
Avian infectious bronchitis virus (IBV) is a highly infectious and contagious pathogen of domestic fowl that replicates primarily in the respiratory tract
but also in some epithelial cells of the gut, kidney, and oviduct. IBV is an enveloped coronavirus (order Nidovirales, family Coronaviridae, genus
Coronavirus) that replicates in the cell cytoplasm and contains an unsegmented, single-stranded, positive-sense RNA genome. In the infected cell,
in addition to the genomic RNA (27kb), IBV produces five subgenomic (sg) mRNAs, each possessing a 64-nt leader sequence derived from the 5′
end of the genome. The signals required for the replication and packaging of the virion RNA are located in the terminal regions of the genome. It is
a highly infectious avian pathogen. When inhaled, virus will attach to glycoprotein receptors containing sialic acid on ciliated epithelial cells of the
respiratory epithelium. The respiratory replication will result in loss of ciliary activity, mucus acumulation, necrosis and desquamation, causing
respiratory distress, râles and asphyxia. Local virus replication will result in viremia, spreading the infection into other tissues and organs. Other
respiratory diseases of chickens (Mycoplasma gallisepticum, avian infectious laryngotracheitis Gallid herpesvirus 1, Newcastle disease, Avian
metapneumovirus infection may be confused clinically to infectious bronchitis. Viremic IBV will also reach the oviduct, causing lesions in the
magnum (the egg-white gland) and in the uterus (the egg-shell gland), leading to a sharp decline of egg production. Infection of chickens at puberty,
during the oviduct development, will impede oviduct formation and destroy future laying capacity, resulting in "false layers".
In general, acute viral infections can be diagnosed by detection of the virus (viral antigens) itself or the specific antibody response viral antigens.
The most common assays for routine use of virus detection are virus isolation (VI), immunofluorescence assay (IFA), immunoperoxidase assay
(IPA), polymerase chain reaction (PCR), and for antibody detection the haemagglutination inhibition (HI) test, agar gel precipitation test
(AGPT/AGID), and enzyme linked immunosorbent assay (ELISA). The virus neutralisation test (VNT) is rarely used for routine diagnosis because it
is relatively expensive and laborious. An assessment of immune status, as well as serological identification of viral infection requires a measurement
of antibody to the virus in serum. Enzyme-linked immunosorbent assay (ELISA) systems have proven efficacious in the quantification of antibody
levels to IBV, and facilitate the monitoring of immune status in large flocks.
ADI’s Chicken Infectious Bronchitis Virus (IBV) ELISA Kit is a simple, rapid, and sensitive method to detect chicken IBV infection or to measure
efficacy of IBV vaccines (V-IBV, P-IBV).
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Rev. 120712A
Alpha Diagnostic Intl Inc., 6203 Woodlake Center Dr, San Antonio, TX 78244, USA; Email: [email protected]
 (800) 786-5777; (210) 561-9515; Fax: (210) 561-9544; Web Site: www.4adi.com