Download Semester 2 2008/2009 - University of Belize

UNIVERSITY OF BELIZE
Faculty of Nursing, Allied Health & Social work
Semester 2 2008/2009
COURSE NAME: APPLIED CLINICAL MICROBIOLOGY
CODE: MLTC 201
CREDITS: 5
DAY & TIME: Lectures - Monday/Wednesday 10:00 - 11:15 a.m.
LABS - Monday/Tuesday/Wednesday 1:00 – 3:00 p.m.
INSTRUCTOR: Trudy Tillett, MPH, MT (Dip. Microbiology, Cert. Medical Technology)
OFFICE HOURS: Tuesday 10:00 – 11:00 a.m.
Phone:(U) 822-3680 ext. 322 (H) 822-0751/203-4267 (C) 624-8164
e-mail address:[email protected]
Pre-requisite: BIOL 402
COURSE DESCRIPTION:
Application of theories and techniques in microbiology to bacterial, mycoplasmal,
rickettsial, chlamydial, viral, parasitic, and mycotic diseases of humans. Topics include
clinical signs and symptoms of these diseases; proper methods of collecting,
transporting, and processing appropriate clinical specimens, laboratory methods that
are used for the identification of these pathogens and diagnosis of the disease they
cause. In the laboratory component, students will process blood, cerebral spinal fluid,
sputum, urine, throat swabs, and stool specimens. Emphasis will be placed on isolation
and identification of bacteria, selection of appropriate antibiotics for therapy, quality
assurance, and laboratory safety.
COURSE OBJECTIVES:
By the end of this course the student should be able to:
 Demonstrate proficiency in aseptic handling of bacteriologic specimens.
 Demonstrate an understanding of bacterial, viral and rickettsial descriptions and
classifications.
 Choose the proper media for and correctly process bacteriologic specimens.
 Identify unknown organisms using techniques practiced in laboratory exercises.





conduct and interpret antimicrobial susceptibility testing
apply the principles of quality assurance in the clinical microbiology laboratory
understand the role of the laboratory in the control of disease outbreaks
demonstrate an understanding of the clinical laboratory technologist's role as a
member of the allied health care team
demonstrate strong ethical and professional values
TEXT:
Required: Mahon, CR, Manuselis, G.. Textbook of Diagnostic Microbiology.
W. B. Saunders Publishers.
Reference: Murray Patrick R. et al.. Medical Microbiology. Mosby Publishers
Other Supplies:
 Laboratory coat
 Felt-tip marking pen
 Wax pencil
 Digital watch/timer – optional
 Pocket calculator - optional
CONTENT/SCHEDULE OF TOPICS:
Week/Date
Lecture Topic
Text References
Assignments
Tests & Quizzes
Laboratory
MODULE 1
Intro to Clinical Microbiology
Chapters: 1 - 13
Week 1
Jan 20 –22
I. Bacterial Cell Structure,
Physiology, Metabolism &
Genetics
II. Host-Parasite Interaction
Chapter 1, pp. 3-21
Assignment: Case Study
p 36
Chapter 2, pp. 28-52
Quiz 1
III. The Laboratory Role in
Infection Control
IV. Control of Microorganisms
Chapter 3 pp. 58-70
Quiz 2
Assignment: Case Study
p 58


Laboratory Safety
Aseptic techniques
V. Performance Improvement in the
Microbiology Laboratory
Chapter 5, pp. 112-13
Quiz 3
Assignment: Case Study
p

Inoculating culture
media
Colonial Morphology
Quiz 4

Week 2
Jan 27–29
Week 3
Feb 3–5
Week 4
Feb 10–12
VI. Specimen Collection & Processing
VII. Microscopic Examination of
Infected Materials
VIII. Use of Colony Morphology for the
Presumptive Identification of
Microorganisms
Chapter 4, pp.73-108
Chapter 6, pp. 134-146
Chapter 7, pp.152-166
Chapter 8, pp. 199-207


Microscopic examination
of bacteria
Using biochemical
testing to identify
bacteria
Week 5
Feb 17–19
IX. Biochemical Identification of
Gram Negative Bacteria
XI. X. Anti biotic Mechanisms of
action and Resistance
XI. Antimicrobial Susceptibility
Testing
Chapter 9, pp. 212-225
Quiz 5
Chapter 12, pp.303-315
Lab Test 1
Chapter 13, pp.319-360
TEST 1


Using biochemical
testing to identify
bacteria
Antimicrobial
Susceptibility
Testing
MODULE 2
Laboratory Isolation of Significant
Isolates
Chapters: 14 – 31
Week 6
Feb 24–26
XII. Staphylococcus
XIII.Streptococci
Chapter 14, pp.367-378
Chapter 15, pp.382-395
Quiz 6

Identification of
Staphylococci
Week 7
Mar3-5
XIV. Corynebacterium
XV. Aerobic Gram Positive Bacilli
XVI. Neisseria & Other Species
XVII. Haemophilus & Other Species
Chapter 16, pp.410-421
Chapter 17, pp.425-431
Chapter 18, pp.438-454
Chapter 19, pp.462-499
Quiz 7

Identification of
Streptococci
Week 8
Mar 10–12
XVIII. Enterobacteriaceae
Chapter 20, pp.502-527
Quiz 8
TEST 2

Identification of
Enterobacteriaceae
Week 9
Mar 17–19
XIX. Vibrio
XX. Non Fermenting & miscellaneous
Gram Negative Bacilli
Chapter 21, pp.542-555
Chapter 22, pp.564-577
Quiz 9

Identification of
Enterobacteriaceae
Week 10
Mar 24–26
XX.
Anaerobes of Clinical
Importance
XXII. Spirochetes
XXIII. Chlamydia, Mycoplasma and
Ureaplasma
XXIV. M. tuberculosis & Other
Mycobacteria
Chapter 23, pp.587-639
Quiz 10


Identification of
Pseudomonas
Identification of
Neisseria
XXV. Medically Significant Fungi
XXVI. Clinical Virology
Chapter 27, pp.718-747
Chapter 29, pp.826-833
Week 11
Mar 31–Apr 2
Apr 7 – 9
Lab Test 2
Chapter 24 pp.642-647
Chapter 25, pp.653-679
Chapter 26, pp.683-714
Quiz 11
Lab Test 3
TEST 3
EASTER BREAK
MODULE 3
Laboratory Diagnosis of Infectious
Diseases
Chapters: 32 – 41
Week 12
Apr 14 – 16
XXVII. Respiratory Infections
XXVIII. Skin & Soft Tissue Infections
XXIX. GI Infections & food Poisoning
Chapter 32, pp. 899-929
Chapter 33, pp. 935-936
Chapter 34, pp. 957-976
Quiz 12

Unknown # 1
Week 13
Apr 21 - 23
XL.
XLI
Chapter 35, pp. 979-990
Chapter 36, pp. 995-1007
.
Quiz 13

Unknown # 2
Week 14
Apr 28 - 30
XLIII. Urinary Tract Infections
XLIV.Sexually Transmitted Diseases
Chapter , pp.1010-1027
Chapter , pp. 1031-1039
Quiz 14

Unknown # 3
Week 15
May 5 – 7
XLV. Laboratory Diagnosis of Fungal
Infections
Chapter , pp 748-757
Quiz 15
TEST 4

Unknown # 4
Week 16
CNS Infections
Bacteremia & Sepsis
PRESENTATIONS
Lab Exam
May 12–14
Final Exam
May 18-25
Method of Instruction:
● Lecture and Power Point Presentations
● Audio-visual Materials
● Laboratory demonstrations and exercises
GRADING:
University of Belize Grading Scheme:
A
95-100
B+
85-89
C+
75-79
D+
65-69
F
0-59
B
C
D
A90-94
80-84
70-74
60-64
Determination of Final Grade
Assignment & Quizzes
5%
Tests Theory
15%
Lab
15%
Exam Theory
30%
Lab
20%
Project
15
TOTAL:
100%
CLASS POLICIES:
Students are expected to attend all lectures, participate in class activities and
discussions, and complete all assignments and laboratory exercises. In addition, the
student must adhere to all established classroom and laboratory policies.Since lecture
material, as well as text material, is covered on exams, absences are likely to adversely
affect performance. Missed exams/tests cannot be made-up without acceptable written
verification for the absence. Missed labs cannot be made-up due to the perishable
nature of many of reagents and supplies.
Attendance and Assignments :
Students are expected to attend ALL lecture and laboratory class meetings, be
on time, and personally take responsibility for their own learning. This includes
completion of all class assignments on the assigned due dates, and attendance on
scheduled test dates.
Dress Code
1.
2.
3.
4.
5.
Students will be expected to attend class clean and neatly dressed.
A laboratory coat must be worn buttoned during all laboratory sessions.
Footwear appropriate for a laboratory setting (closed-toe) will be required.
Hair that is shoulder length or longer must be worn up or securely tied back.
Loose or dangling jewelry will not be permitted.
Safety Regulations
Proper Hand washing Procedure
a. Wet hands and apply a small amount of an antiseptic soap (3-5 mL).
b. Vigorously lather hands and rub together for at least 15 seconds.
c. Wash well between fingers and up the wrists.
d. Rinse well with a moderate stream of water in a downward motion.
e. Dry with a paper towel and use the towel to turn off the faucet.
Laboratory Requirements
It is the responsibility of the student to prepare for each lecture/laboratory session. Each
student is responsible for his/her own work station. The instructor reserves the right to
give an unannounced quiz at any time.
Blood and fecal samples possibly containing pathogenic organisms will be used in this
course; therefore, universal precautions must be observed.
1. Eating, drinking, or smoking in the laboratory is strictly prohibited. Avoid putting
objects in your mouth.
2. Wear gloves when working with unpreserved body specimens.
3. Hands must be washed when leaving the laboratory for any reason.
4. The working area must be thoroughly disinfected after each use with 10% bleach
solution.
5. Spills must be disinfected by covering with paper towels, soaking thoroughly with
disinfectant and waiting 15 minutes before cleaning.
6. All accidents are to be reported immediately to the laboratory supervisor/instructor.
General Comments:
Most of the laboratory exercises will take 2 or 3 lab periods to complete. It is therefore
necessary to bring lab notebooks, which contain previous documentation of
observations etc., to every laboratory period.
It is the responsibility of the student to prepare for each lecture/laboratory session. Each
student is responsible for his/her own work and for the cleaning up of their work station.