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BIOLOGICAL CLASSIFICATION BIOLOGICAL CLASSIFICATION Prerequisites Learning Objectives • Kingdom Monera • Kingdom Protista • Kingdom Fungi • Kingdom Plantae • Kingdom Animalia • Viruses, Viroids and Lichens Prerequisites Before studying this chapter, you are required to know the following concepts as pre-requisites for better understanding of this chapter: 1) Clear understanding of diversity in the living world. 2) Basic understanding of biology and biological concepts. 3) What is classification and why it is required in any field. 4) What is the meaning of a ‘kingdom’ and 5) Different taxonomic categories and the position of taxonomic category “Kingdom”. 10 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Learning Objectives After completing this chapter you will be able to know and answer the following concepts: 1) Why classification is required 2) Two kingdom classification 3) Five kingdom classification 4) Merits of five kingdom classification 5) Kingdom Monera 6) Kingdom Protista 7) Kingdom Fungi 8) Kingdom Plantae 9) Kingdom Animalia and 10) Viruses, Viroids and Lichens. Let us study the above concepts in brief with some examples. Introduction In the last chapter, we studied “Diversity in the living world”. As we knew that life on earth is famous for its diversity. There are millions of different types of organisms living in this world. Hence, they need to be classified into different groups to help identify them. The biggest groups into which living organisms are classified into are called “kingdoms”. Aristotle was the earliest to attempt a more scientific basis for classification. Two Kingdom Classification: Two kingdom system of classification was developed during Linnaeus’ time. These two kingdoms are Plantae and Animalia which include all plants and animals respectively. This system did not differentiate prokaryotes and eukaryotes, unicellular and multicellular organisms and photosynthetic (green algae) and non-photosynthetic (fungi) organisms. This system included a large number of organisms which does not fit into either category. Hence it was found to be inadequate. Aristotle two kingdom Classification kingdom kingdom Plantae Animalia 11 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Five Kingdom Classification: It was proposed by R.H. Whittakar (1969). The five kingdoms defined by him are: Kingdom Monera, Protista, Fungi, Plantae and Animalia. The main criteria for his classification are cell structure, mode of nutrition, reproduction, thallus organization and phylogenetic relationships. See the table for comparative understanding of different characteristics of the five kingdoms. Monera R.H. Whittakar Five Kingdom Classification Animalia Protista Plantae Fungi Characters Cell type Cell wall Five Kingdoms Monera Procariotic Protista Fungi Eucariotic Eucariotic Non cellular Present (Polysaccharide) Present in some (without cellulose) + amino acid Plantae Eucariotic Eucariotic Present (cellulose) Absent Nuclear membrane Absent Present Present Present Body organisation Cellular Cellular Multicellular/ loose tissue Tissue/ organ Autotrophic (chemosynthetic and Photosynthetic) and Heterotrophic (saprophyte/ parasite) Autotrophic (Photosynthetic and Heterotrophic Mode of nutrition 12 Heterotrophic (Saprophytic/ Parasitic) Animalia Autotrophic (Photosynthetic) Present Tissue/organ/ organ system Heterotrophic (Holozoic/ Saprophytic etc) www.sciencetuts.com BIOLOGICAL CLASSIFICATION Benefits of Five Kingdom Classification: 1) Earlier classifications placed prokaryotic bacteria and the blue green algae with other groups which are eukaryotic. This classification separated prokaryotes from eukaryotes. 2) All unicellular eukaryotic organisms are kept in one group. 3) Differentiated heterotrophic fungi from autotrophic green plants. 4) It is based on evolutionary relationships. In this chapter, we shall study the characteristics of kingdom Monera, Protista and Fungi of five kingdom classification. KINGDOM MONERA Kingdom Monera includes Bacteria which most abundant micro-organisms. Bacteria live in extreme habitats like hot springs, deserts, snow and deep oceans. Prokaryotes are bacteria which are unicellular and have no distinct nucleus. They have a naked DNA. Structure is simple but behaviour is complex. They show the most extensive metabolic diversity. Some of the bacteria are autotrophic i.e. they prepare their own food material from inorganic substances. Vast majority of bacteria are heterotrophic i.e. they do not synthesize their own food but depend on other organisms or on dead organic matter for food. Bacteria Categories of Bacteria: Bacteria are grouped into 4 categories based on their shape. They are 1. The Spherical Coccus (plural: cocci) 2. The Rod-shaped Bacillus (plural: bacilli) 3. The comma-shaped Vibrium (plural: vibrio) and 4. The Spiral-shaped Spirillum (plural: spirilla). Bacteria Coccus Bacillus Vibrium Spirillum Categories of Bacteria 13 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Archaebacteria: These are special type of bacteria live in most harsh habitats such as extreme salty areas (halophiles), hot springs (thermoacidophiles) and marshy areas (methanogens). The reason for living in most harsh habitats is because of having a different cell wall structure. The production of methane (biogas) from the dung of ruminant animals such as cows and buffaloes is because of the presence of methanogens kind of bacteria in their gut. Archaebacteria Eubacteria: These are true bacteria characterized by the presence of a rigid cell wall and a flagellum, if motile. These are either autotrophs or heterotrophs based on how they synthesize their food. Cyanobacteria (or) blue-green algae have chlorophyll similar to green plants called photosynthetic autotrophs. Eubacteria Cyanobacteria: These are true bacteria characterized by the presence of a rigid cell wall and a flagellum, if motile. These are either autotrophs or heterotrophs based on how they synthesize their food. Cyanobacteria (or) blue-green algae have chlorophyll similar to green plants called photosynthetic autotrophs. The cyanobacteria are unicellular, colonial or filamentous, marine or terrestrial algae. The colonies are surrounded by gelatinous sheath. Some of these bacteria can fix atmospheric nitrogen inspecialized cells called heterocysts. Examples: Nostoc and Anabaena. Hetirosysts Cyanobacteria Nostoc Anabaena Some bacteria which oxidize various inorganic substances like nitrates, nitrites and ammonia for their ATP production are called chemosynthetic autotrophic bacteria. They play major role in recycling nutrients like nitrogen, phosphorous, iron and sulphur. Chemosynthetic autotrophic bacteria 14 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Heterotrophic bacteria are the most abundant in nature. These act as decomposers and helpful in making curd from milk, production of antibiotics, fixing nitrogen in legume roots etc. Some heterotrophic bacteria are pathogens causing diseases in human beings, crops, farm animals and pets. The method of reproduction in bacteria is fission. The smallest living cells without a call wall and survive without oxygen called mycoplasmas. These are pathogenic in animals and plants. Heterotrophic bacteria Mycoplasmas Binary Fission in Bacteria KINGDOM PROTISTA These are single-celled eukaryotes. They contain a well defined nucleus and other membrane bound organelles. Algae and Protozoa are included in this kingdom. Mostly unicellular or simple multi-cellular. These members live in water i.e. aquatic in nature. This kingdom forms a link with other kingdoms dealing with plants, animals and fungi. Protists reproduce asexually and sexually by a process involving cell fusion and zygote formation. In this module, we include the following groups under kingdom Protista: 1. Chrysophytes 2. Dinoflagellates 3. Euglenoids 4. Slime moulds and 5. Protozoans. We shall see some characteristics with examples for the above groups. 15 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Kingdom Protista Chrysophytes Dianoflagellates Euglenoids Slime moulds Protozoans Kingdom Protista 1. Chrysophytes: It includes diatoms and golden algae (desmids) found in fresh water as well as marine water. They are microscopic and float passively in water currents (plankton). In diatoms, the cell walls form two thin overlapping shells. The walls are embedded with silica and thus the walls are indestructible and left behind in large quantities. This accumulation over billions of years is referred to as ‘diatomaceous earth’.Diatoms are chief producers in the oceans. Diatomacious Earth Examples of Diatoms 2. Dinoflagellates: These are mostly marine and photosynthetic appear in different colors like yellow, green, brown, blue or red depending on the main pigments present in their cells. The presence of stiff cellulose plates on the outer surface of cell wall and two flagella is a characteristic feature of dinoflagellates. One flagella lies longitudinally and the other transversely in a furrow between the wall plates. Example: Gonyaulax (Red dianoflagellate) – These multiply very rapidly and make the sea appear red (red tides). 16 Dinoflagellates www.sciencetuts.com BIOLOGICAL CLASSIFICATION Gonyaulax (Rad dinoflagellate) 3. Euglenoids: These are fresh water protists found in stagnant water. No cell wall but pellicle is present which makes their body flexible. Two flagella are present – a short and a long one. In the presence of sunlight they are photosynthetic in nature, otherwise they act as heterotrophs.The pigments present in euglenoids are similar to those present in higher plants. Example: Euglena. 4. Slime Moulds: These are saprophytic protists. The body moves along decaying twigs and leaves engulfing organic material. They form an aggregation called plasmodium under suitable conditions and spread over several feet. During unfavourable conditions, the plasmodium differentiates and forms fruiting bodies bearing spores at their tips. The spores are highly resistant and dispersed by air currents. long flagella short flagella Pellicle Euglena fruiting body Smile Moulds 5. Protozoans: All protozoans are heterotrophs and live as predators or parasites. There are four major groups of protozoans: 1. Amoeboid protozoans – Example: Amoeba and Entamoeba 2. Flagellated protozoans – Example: Trypanosoma 3. Ciliated protozoans – Example: Paramoecium and 4. Sporozoans – Example: Plasmodium (Malaria parasite). Trypanosoma Paramecium 17 Amoeba Entamoeba Plasmodium www.sciencetuts.com BIOLOGICAL CLASSIFICATION KINGDOM FUNGI We commonly observe rotten oranges, moulds on the bread in our daily life. These are all because of fungi. The common mushroom we eat and toadstools are also fungi. White spots on the mustard leaves are due to a parasitic fungus. Oranges Leaf Bread Yeast, unicellular fungi, is used to make bread and beer. Some fungi cause diseases in plants and animals. For example; Puccinia causes rust disease in the wheat. Fungi are cosmopolitan but they prefer to grow in warm and humid places. Fungi have filaments. Fungi bodies have long, slender thread like structures called hyphae. The network of hyphae is known as mycelium. Coenocytic hyphae are continuous tubes filled with multinucleated cytoplasm. Fungi Mycelium The cell walls of fungi are composed of chitin and polysaccharides. Moulds, yeasts and mushrooms are included in the kingdom fungi. These are heterotrophic eukaryotes with a chitin cell wall. They are also saprotrophs, meaning they get their food from the decomposing matter. Some fungi depend on plants and animals for their food are called parasites. They also live as symbionts – in association with algae as lichens and with roots of higher plants as mycorrhiza. Reproduction in fungi is by both sexual and asexual methods. Fragmentation, fission and budding are the vegetative means of reproduction. Above figures in association with algae as lichens and with roots of higher plants as mycorrhiza Asexual reproduction is by spores called conidia or sporangiospores or zoospores whereas sexual spores are oospores, ascospores and basidiospores. These spores are produced in specialized structures called fruiting bodies. 18 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Reproduction in fungi vegetative reproduction Asexual reproduction sexual reproduction Fragmentation Conidia oospores Fission sporangiospores ascospores budding zoospores basidiospores The sexual cycle involves three steps as follows: 1. PLASMOGAMY: Fusion of protoplasms between two motile or non-motile gametes. 2. KARYOGAMY: Fusion of two nuclei. 3. SPORES FORMATION: Meiosis in zygote resulting in haploid spores. Examples of Sexual cycle in Fungi Classification of the Kingdom FUNGI: Kingdom Fungi classified into the following classes based on the morphology of mycelium, mode of spore formation and fruiting bodies. Fungi Phycomycetes Basidiomycetes Ascomycetes Deuteromycetes Classification of the Kingdom Fungi Phycomycetes: Members belong to phycomycetes live in aquatic habitats and decaying wood in moist places. They also live as obligate parasites on plants. The mycelium is aseptate and coenocytic. Asexual reproduction is by zoospores (motile) or by aplanospores (non-motile). These are produced endogenously in sporangium. Zygospores are formed by fusion of two gametes. These gametes are isogamous or anisogamous. Examples: Mucor, Rhizopus (Bread mould) and Albugo (Parasitic fungi on mustard) 19 www.sciencetuts.com BIOLOGICAL CLASSIFICATION Mucor Rhizopuses Albugo Ascomycetes: These are also known as sac-fungi. These are unicellular or multicellular in nature. Example for unicellular ascomycetes is yeast (Sacharomyces). Example for multicellular ascomycetes is Pencillium.These are saprophytic, decomposers, parasitic or coprophilous (growing on dung). Mycelium is branched and septate. Yeast Pencillium The asexual spores are conidia which are produced on conidiophores. Conidia on germination produce mycelium. Ascospores are sexual spores produced in a sac like asci (singular ascus) which are arranged in fruiting bodies called Ascocarps. Examples: Aspergillus, Claviceps and Neurospora. Neurospora is extensively used in biochemical and genetic work. Aspergillus Claviceps Neurospora Basidiomycetes: Mushrooms, bracket fungi or puff balls are common members of basidiomycetes. These fungi cause plant diseases called rusts and smuts. Asexual spores absent. Vegetative reproduction by fragmentation is common. Special sex organs are absent. The fusion of two vegetative cells of different strains results in plasmogamy which results in dikaryotic basidium (plural basidia). Karyogamy and meiosis takes place inside basidium resulting in four basidiospores. Basidia are arranged in fruiting bodies called Basidiocarps. Examples: Agaricus (Mushroom), Ustilago (Smut), Puccinia (Rust fungus). Agaricus 20 Ustilago www.sciencetuts.com BIOLOGICAL CLASSIFICATION Deuteromycetes: These are commonly known as imperfect fungi because only the asexual or vegetative phases are known. Once perfect (sexual) stages were known, they often moved to ascomycetes and basidiomycetes. Mycelium is branched and septate. The asexual spores are conidia. A large number of members are decomposers on litter and help in mineral cycling. Examples: Alternaria, Colletotrichum and Trichoderma. Alternaria Colletotrichum Trichoderma KINGDOM PLANTAE It includes chlorophyll containing multi-cellular eukaryotes called plants. Cell wall is present and made up of cellulose. Most of the members are autotrophic means that they prepare their own food material. A few members are partially heterotrophic such as the insectivorous plants or parasites. Insectivorous plants or Parasites Examples for insectivorous plants are – Bladderwort and Venus fly trap. Example for a parasitic plant – Cuscuta. Life cycle shows alternation of generation by having haploid gametophytic and diploid saprophytic phases. You will study further details in the next chapters. Dionaea 21 Cuscuta www.sciencetuts.com BIOLOGICAL CLASSIFICATION KINGDOM ANIMALIA It includes heterotrophic multi cellular eukaryotes called animals. Cell wall is absent. Depend on plants directly or indirectly for food. Stores food reserves as fat or glycogen. The mode of nutrition is holozoic. These are capable of locomotion. Reproduction is sexual. Further details can be studied in the coming chapters. Multi cellular Eucaryotes VIRUSES, VIROIDS AND LICHENS Viruses: Viruses are not placed in a proper kingdom as they are not truly ‘living’. Viruses are non-cellular organisms and they have an inert crystalline structure outside the living cell. TMV Vaccinia virus Adeno virus Polio virus Rabies virus The name ‘virus’ means venom or poisonous fluid was given by Pasteur. D.J. Ivanowsky (1892) when he recognized certain microbes as casual organism of the mosaic disease of tobacco. M.W.Beijerinek (1898) extracted a fluid called Contagium vivum fluidum (infectious living fluid) which cause infection in healthy plants. W.M. Stanley (1935) crystallized the viruses and shown that crystals consist largely of proteins. Viruses are obligate parasites as they are inert outside their specific host cell. Pasteur. D.J. Ivanowsky (1892) M.W. Beijerinek (1898) 22 W.M. Stanley (1935) www.sciencetuts.com BIOLOGICAL CLASSIFICATION Body has protein coat called capsid and genetic material is DNA or RNA. No virus contains both RNA and DNA. Plant viruses have single stranded RNA. Animal viruses have double stranded DNA or RNA. Animal diseases like mumps, herpes, small pox, influenza and AIDS are caused by viruses. Plant diseases like mosaic, leaf rolling, curling and yellowing of leaves is caused by viruses. Rabies virus RNA TMV Bacteriophage Viroids: Discovered by T.O.Diener in 1971. Smaller than virus and lack protein coat, hence called viroids. It has a free RNA of low molecular weight. It caused potato spindle tuber disease. T.O.Diener (1971) Lichens: The mutually beneficial symbiotic association between algae and fungi is known as lichens. The algal component is known as phycobiont and fungal component as mycobiont which are autotrophic and heterotrophic respectively. Algae prepare food for fungi and fungi provide shelter and absorb mineral nutrients and water for its algal component. Lichens do not grow in polluted areas and hence they are very good pollution indicators. Lichens algal component (Phycobiont) fungal component (Mycobiont) Autotrophic Heterotrophic Lichens 23 www.sciencetuts.com BIOLOGICAL CLASSIFICATION SUMMARY • • • • • • Aristotle was the earliest to attempt a more scientific basis for classification based on morphological characters. Two kingdom classification was proposed by Linnaeus. He divided all the living organisms into two kingdoms namely Plantae and Animalia. Five kingdom classification was proposed by Whittakar. They are Monera, Protista, Fungi, Plantae and Animalia. The main criteria for five kingdom classification were cell structure, body organization, mode of nutrition , reproduction and phylogenetic relationships. Kingdom Monera includes bacteria which are prokaryotic in nature. Bacteria are unicellular without distinct nucleus. Bacteria may be autotrophic or heterotrophic in their mode of nutrition. Kingdom Protista includes single-celled eukaryotes. Algae and Protozoa are included in this kingdom. These are aquatic in nature. Kingdom fungi include moulds, yeasts and mushrooms. Fungi are heterotrophic eukaryotes with a chitin in their cell wall. Most fungi are saprophytic in their mode of nutrition. Phycomycetes, Ascomycetes, Basidiomycetes and Deuteromycetes are the four classes included in the kingdom fungi. 24 www.sciencetuts.com