Download (BSL-3) lab to an Arthropod Containment Level 2

Conversion of a Biosafety Level 3 (BSL-3) lab to
an Arthropod Containment Level 2 (ACL-2) lab
Jolly Qian, Biosafety/ACL-2 ops manager, PhD RBP
Jason Khoo, Head of HSE and facilities
Lakshmanan Ponnusamy, facility supervisor
Novartis Institute for Tropical Diseases (NITD)
NITD @ Biopolis Singapore
(Proteos)
Nanos: Institute of
Institute of Molecular
and Cell Biology
Bioengineering
& Nanotechnology
Matrix:
BioInformatics
Institute
Helios
Genome
Centros:
Carpark
‘A’
Min. of
Education
Bioprocessing
Technology Centre
Institute of
Singapore
Chromos
NITD
NITD to move to Biopolis in April 2004 with official opening in July 2004
Conversion of BSL-3 to ACL-2
 Decommission of BSL-3 (skip)
 Planning of an ACL-2 lab
• Space- how many rooms do we need?
• Conversion-how to deal with the M&E works?
• Staffing-operation
• Science
 Construction of the ACL-2 lab
3 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Why ACL-2 (Arthropod Containment Level-2)
 Support the Malaria drug discovery program
 Reference ‘ACME’
I. Arthropod Containment Guidelines by The American
Committee of Medical Entomology of the American
Society of Tropical Medicine and Hygiene
II. Arthropod Containment Level 2 (ACL-2) must be
practiced if working with exotic and indigenous
arthropods infected with BSL-2 agents associated with
animal and/or human disease, or that are suspected of
being infected with such agents.
4 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
ACL-2 lay-out
Animal room
Feeding room
Dissection room
Infectious Area
Anteroom Ana
2
Anteroom 1
HighBreeding
Temproom
& Humidity
5 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Configuration of BSL3 HVAC system
Non- Return Damper
Isolation Damper
Balancing Damper
AHU-8-2
NonReturn
Damper
EF8-2
EF8-1
AHU-8-1
•Isolation
Damper
BSL-3+/ABSL-3
Laboratory
6 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Separate supply air only for the breeding room
Animal room
Feeding room
Dissection room
Infectious Area
Anteroom Ana
2
Anteroom 1
HighBreeding
Temproom
& Humidity
7 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Conversion
 Retained most of the BSL-3 infrastructures e.g. ACMV,
gowning rooms, clean corridor area etc. etc.
 Except for the breeding room, which used a FCU to
supply air, still using the old BSL-3 AHU/Exhaust->single
pass, high ACH
 Bag-in Bag-out system ×
 Brand new and white lab furniture, lighting system under
benches
 Installed two humidifiers for the breeding room to back-up
each other
8 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Comparison of BSL-3 and ACL-2 (differences and
similarities)
 Different type of risks  difference in Risk Assessment
BSL-3
Room filter
HEPA filter
ACL-2
Air exhaust and supply covered with fine
mesh, including structures like sprinklers
etc.
Room Pressure Negative
Slightly negative
Waste disposal Decontamination
tank and passthrough autoclave
A small stand-alone autoclave for solid
waste and a ‘rice cooker’ for non-infectious
liquid waste like larvae culture water
Safety
equipment
BSC/Glovebox/
Respiratory
protection program
Glovebox (no ventilation!) and mosquito
traps
9 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
How to contain a tiny mosquito- facility features
Sliding doors with alarm
Air curtain
Feeding room
Air curtain
Animal room
Sliding doors with alarm
Dissection room
glovebox
Anteroom Ana
2
Interlock
doors
Anteroom 1
Breeding room
Lower Ceiling
Controlled access
With air curtain
Physical curtain
10 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Prevention of Mosquito Escapees
 Non-infected mosquito escapees
• Not very unusual but it can be serious too
• How and where does it happen? Look at the details in the procedure
e.g. change glucose etc etc
 Infected mosquito escapees
• Mosquito infection happens in strictly controlled environment so
escapee should be very rare and serious case
• Evacuation
• Review each step in the infection and dissection process and plan in
advance
• Perform mock experiment with non-infected mosquitoes
-be familiar with the procedure
-build up confidence!!!
11 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Prevention of Mosquito Escapees contd.
 Regular check of mosquito traps it is good to have more
than one trap for a relatively bigger room!
 Regular (quarterly) change of attractant in the traps
 Understand the mosquito life cycle
12 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Insectary Safety and Operational Challenges
 Staffing
• Back-up system absolutely necessary!
 Weekend/holidays duties
- directly impact the safety standard
 Working environment
- Hot and humid
- Low ceiling and no window
13 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Insectary Operational Challenges contd.
Housekeeping is crucial for both of safety and scientific
purpose!!!
• No clutter
• freeze the dirty trays or cages and then wash them immediately
• mop floor frequently
• regular change of lab coat
14 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only
Thank for your attention!
NITD insectary team and our collaborators (Duke-NUS and Environmental Health Institute):
Andy Yip, Lim Liting, Pablo Bifani and Sudirman Made Ali Rusdiyah
Novartis Institute For Tropical Diseases Management
15 | BSL3 to ACL2 | Jolly Qian | Oct 14 2015 | Biosafety | Business Use Only