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Copyright Notice! This PowerPoint slide set is copyrighted by Ross Koning and is thereby preserved for all to use from plantphys.info for as long as that website is available. Images lacking photo credits are mine and, as long as you are engaged in non-profit educational missions, you have my permission to use my images and slides in your teaching. However, please notice that some of the images in these slides have an associated URL photo credit to provide you with the location of their original source within internet cyberspace. Those images may have separate copyright protection. If you are seeking permission for use of those images, you need to consult the original sources for such permission; they are NOT mine to give you permission. ECSU Biology Club [email protected] Meetings: Tuesdays, 5 PM, Goddard Lobby House Party 9 PM November 13 Student Center Theater Food etc. Tropical Biology (Costa Rica) Biology 320 May 20-31, 2008 Register for Bio 360 and 320 for Spring $1900 approximate cost Scholarships Available! For more information: Dr. Elliott or Dr. Szczys G113 or Planetarium EML Spring 2008 Registration Advising Go to the office of your academic advisor… do not telephone her/him! Danielle, Heather, Carlos: Media 224 Make an appointment… usually by sign-up sheet posted on the door Freshmen (<30 cr): November 26-30 Sophomores (30-<60 cr): THIS WEEK! Disposing of Wastes Regulation of body fluids Plant cells respond to their environmental solution plasmolysis http://botanika.biologija.org/zeleniskrat/slike/slike_drobnogled/Elodea/Elodea_list02.jpg The plant cell wall prevents bursting. A plant cell is normally bathed in a very hypotonic solution. It takes in water until the cell is full. A plant cell placed in a hypertonic solution loses water. Ultimately outward flow stops when the cytosol concentration matches that of the solution. µg element ml-1 sap Yearly changes in nitrogen and potassium concentrations in xylem sap of apple trees in New Zealand 200 blossom time K 160 fruit harvest 120 80 N 40 0 Aug Oct Dec Feb Apr Jun sampling date The range of concentrations are far greater than animal cells could tolerate The capillaries of the stomach and intestine absorb enough nutrients that would swamp animal cells. ©1996 Norton Presentation Maker, W. W. Norton & Company The concentrations of nutrients are regulated by the human liver The circulation via the portal vein goes to the capillaries in the liver. These regulate blood concentration. Blood Glucose The vertebrate liver absorbs excess glucose (forming glycogen) high And it releases that glucose when needed later entering liver leaving liver normal low meal rest exercise Time (hours) This is a basic example of homeostatic regulation The liver: • Regulates blood glucose levels via glycogen. • Converts fermentation-produced lactic acid into glycogen. • Interconverts carbohydrates into fats, conversions of fats, and amino acids into carbohydrates or fats. • Deaminates amino acids and converts the resulting ammonia into urea and uric acid and releases these nitrogenous wastes into the bloodstream. NH3 ammonia O NH2 O=C NH2 urea NH HN =O O NH NH uric acid • Detoxifies a wide range of toxic chemicals including alcohol. • Produces blood plasma proteins: fibrinogen, prothrombin, albumin, globulins…recycles aging red blood cells • Produces bile for fat emulsification. Ion concentration in sea water and body fluids (mM) Na+ Ca2+ K+ Mg2+ Cl- 470 9.9 10.2 53.6 548 Jellyfish (Aurelia) 454 10.2 9.7 51.0 554 Sea urchin (Echinus) 444 9.6 9.9 50.2 522 Lobster (Homarus) 472 10.0 15.6 6.8 470 Crab (Carcinus) 468 12.1 17.5 23.6 524 Mussel (Anodonta) 14 0.3 11.0 0.3 12 Crayfish (Cambarus) 146 3.9 8.1 4.3 139 161 7.9 4.0 5.6 144 Honeybee (Apis) 11 31.0 18.0 21.0 -- Japanese beetle (Popillia) 20 10.0 16.0 39.0 19 Chicken (Gallus) 154 6 5.6 2.3 122 Human (Homo) 140 4.5 2.4 0.9 100 Sea Water http://www.pacificislandbooks.com/aurelia.jpg Marine invertebrates Freshwater invertebrates Terrestrial animals Cockroach (Periplaneta) What conclusion do you draw from this? Osmotic concentration of body fluids Which invertebrate shows osmotic regulation? Carcinus Maia http://www.peixosdepalamos.com/img/p roductes/fitxa_productes/cabra.jpg http://www.marine.csiro.au/crimp/images/ NIMPIS/Carcinus_maenas2.jpg Nereis http://upload.wikimedia.org/wikipedia/commo ns/thumb/1/18/Nereis_succinea_(epitoke).jpg /800px-Nereis_succinea_(epitoke).jpg Salt Water Brackish Water Fresh Water Osmotic concentration of medium Quiz 11--Lauryn Bonanno please see me. One student missed only two questions, but I adjusted each earned score by three questions. So the 94.1% was increased to 102.9%. Two people therefore were at 100% or better…Congratulations! The average quiz score after adjustment was 74.2% The current average of course averages is 78.7% so we are still basically at the B/C border. We are slipping a bit, however, and I attribute that change to the increasing number of late lab papers and the penalties associated with them. Please don’t torpedo your own grades. Plantae: Vegetative due today before 5 PM. First Draft of Term Project due Monday! If received the following Monday (after Thanksgiving) it will be a 70% penalty! OUCH! Jodi Lavoie please see me. Ion concentration in sea water, body fluids, and fresh water (mM) Na+ Ca2+ K+ Mg2+ Cl- 470 9.9 10.2 53.6 548 Brown trout (Salmo) 144 6.0 5.3 -- 151 Crayfish (Cambarus) 146 3.9 8.1 4.3 139 Mussel (Anodonta) 14 0.3 11.0 0.3 12 0.65 0.01 0.2-5.0 0.2 0.5 Sea Water Freshwater http://www.flyandfield.com/newimages/desig n3/pictures/eastlake-brown-big.jpg Freshwater organisms What conclusion do you draw from this? http://io.uwinnipeg.ca/~simmons/16cm05/1116/bluegill4.jpg in hypotonic medium salts water salts dilute urine Saltwater Blue-spotted Grouper (Cephalopholis argus) in hypertonic medium salts+ water water salts isotonic urine http://upload.wikimedia.org/wikipedia/commons/thumb/3/34/Bluespotted.grouper.arp.jpg/793px-Blue-spotted.grouper.arp.jpg Freshwater Bluegill (Lepomis macrochirus) Fluid elimination per minute (µm3/100µm3 of protoplasm) 7 6 5 4 contractile vacuole 3 2 0 10 20 30 40 Osmotic concentration of medium (% of seawater concentration) http://www.microscopy-uk.org.uk/mag/imagsmall/amoebafeeding3.jpg Amoeba proteus 1 0 50 water salts ©1996 Norton Presentation Maker, W. W. Norton & Company Contractile vacuole filling The vacuole moves to the cell membrane and empties by exocytosis full emptying empty ©1996 Norton Presentation Maker, W. W. Norton & Company Paramecium food vacuole Land Planaria QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. Bipalium Cryptic Coloration: hiding strategy Marine Planaria QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. http://entweb.clemson.edu/cuentres/eiis/factshot/images/landplanaria.jpg Aposematic Coloration: warning strategy QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. http://www.tropicaldesigns.com/june05/Aposematic-lg.jpg http://marenostrum.org/nuestrascostas/cbbuceo/puntaferro/fotoslluisalbert/planaria.jpg NH3 Na+ H2O ©1996 Norton Presentation Maker, W. W. Norton & Company Planaria excretory system Flame cell Lumbricus terrestris http://iris.cnice.mecd.es/biosfera/alumno/1bachillerato/animal/imagenes/nervio/lumbricus.jpg ©1996 Norton Presentation Maker, W. W. Norton & Company Each earthworm segment has its own nephridium Earthworm (Lumbricus) nephridium Na+ H2 O Ion pumping removes Na+ Reabsorption into Water follows capillaries osmotically nephrostome Concentrated urine empties through the NH 3 outside body wall nephridiopore NH3 Na+ H2 O Pressure forces coelomic fluid into opening Polyplacophora: chitons The most-primitive mollusc has 8 valves (plates) protecting its soft tissues beneath. The chiton foot attaches to rocks and the animal uses its radula to scrape organic material from the rock surfaces. http://www.dec.ctu.edu.vn/sardi/mollusc/images/chiton.jpg http://www.birdsasart.com/red%20Chiton.jpg This cartoon shows a longitudinal slice of a chiton with the three principal parts: foot (locomotion or attachment), visceral mass (internal organs), and mantle (secretes valves). dorsal aorta gonad valve plates heart pericardial cavity (coelom) ventricle hemocoel auricle radula mantle mouth anus foot digestive stomach nephridium nephridiopore gland ventral gonopore nerve cord (not shown) How does a bivalve eliminate waste? http://www.nmfs.noaa.gov/prot_res/images/other_spec/scallop_eyes.jpg This cartoon is shows a plane of section perpendiular to the photo. The foot can push a bivalve through sediments. The food-trapping gills are used for gas exchange. The heart pumps the blood into the hemocoel bathing the tissues. It goes through the gills for gas exchange. The blood then returns to the heart. hinge and ligament shell heart nephridium intestine mantle gonad gills foot Nephridia cleanse the blood of nitrogenous waste. Insects use Malpighian tubules for waste elimination Malpighian tubules hindgut (intestine) midgut crop anus rectum salivary gland http://www.ibdhost.com/demo/gallery/albums/bugs/grasshopper.jpg mouth ©1996 Norton Presentation Maker, W. W. Norton & Company Because insects have an open circulation system… Waste elimination is more tied to digestion than to circulation prostate ©1996 Norton Presentation Maker, W. W. Norton & Company The renal excretory system in a male human (Homo sapiens) Females do not have a prostate valve and have a shorter urethra Longitudinal section diagram of a human kidney renal circulation system renal functional system ©1996 Norton Presentation Maker, W. W. Norton & Company filtration and concentration unit for blood collection and ducting Nephron Structure and Function: similar to a nephridium renal cortex renal medulla ©1996 Norton Presentation Maker, W. W. Norton & Company to renal pelvis Glomerulus function: the capillary leaks water, ions, and waste molecules into Bowman’s capsule ©1996 Norton Presentation Maker, W. W. Norton & Company ©1996 Norton Presentation Maker, W. W. Norton & Company Glomerulus structure: the proteins and blood cells are retained, but water, electrolytes and other small molecules are filtered out. Bowman’s proximal capsule tubule • filtration • osmosis of water 1 3 4 6 8 10 12 H2O descending loop of Henle • ducting for ammonia and uric acid elimination cortex Na+ Cl- collecting duct • concentration of urine solute concentration in hundreds of milliosmoles per liter • active and passive recovery of salt distal tubule ascending loop of Henle Functions of the nephron: outer medulla Na+ Cl- inner medulla urea to renal pelvis Active transport of Na+ against its concentration gradient Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ phospholipid bilayer K/Na antiport ATPase transport protein Na+ ©1996 Norton Presentation Maker, W. W. Norton & Company Na+ Na+ + Pi Na+ This is obviously not only active transport but also an antiport system Plantae: Vegetative The highest score was 104%. Congratulations! Six people earned 100% or more! Congratulations! The average on the exercise was 96.6%. Congratulations! This average does not include the four papers that were late. Your score will be included in the course average update on Quiz 12 to be given next week on Thursday. Pictogram of movement through the nephron Bowman’s capsule salt proximal tubule water distal tubule urea collecting duct loop of Henle to renal pelvis Nephron: renal capillaries recover sodium and water into the blood after filtration of small molecules proximal tubule Bowman’s capsule distal tubule renal artery glomerulus renal vein loop of Henle collecting duct ureter