Download YR56 Virology Rate Sheet.xlsx

Oregon National Primate Research Center
Molecular Virology Support Core (MVSC) Rates 2015-2016
For reduced F&A, enter amount of F&A in field for calculations:
Service
Adeno-Associated Virus (AAV):
2
AAV VECTOR MEDIUM-SCALE PRODUCTION (2,000 cm culture scale, yields depend on serotype,
typically ranging between 1011 to 1013 viral genomes (vg), iodixanol step-gradient purification, incl. purity
assessment and viral genome titer)
2
AAV VECTOR LARGE-SCALE PRODUCTION (6,000 cm culture scale, yields depend on serotype,
typically ranging between 1012 to 1014 viral genomes (vg), iodixanol step-gradient purification, incl. purity
assessment and viral genome titer)
AAV VECTOR "MINI-PREP" (150 cm2 culture scale, yields depend on serotype, typically ranging
between 1010 to 1012 viral genomes (vg), buffer-exchanged, concentrated, partially-purified viral lysate,
incl. viral genome titer)
47%
Comments
Unit
Item Code
Federal
Reduced F&A
Industry
Yields are not guaranteed and
vary between individual preps
Lot
AVMPS
$1,203.00
$1,203.00
$2,872.16
Yields are not guaranteed and
vary between individual preps
Lot
AVLPS
$2,329.00
$2,329.00
$5,560.49
Yields are not guaranteed and
vary between individual preps
Lot
AVMIN
$100.00
$100.00
$238.75
MicroLiter
AVPS
$1.00
$1.00
$2.39
Sample
AVNET
$45.00
$45.00
$107.44
Sample
AVNES
$15.00
$15.00
$35.81
Sample
Sample
Sample
AVPME
AVIUT
AVENT
$245.00
Quote
Quote
$245.00
Quote
Quote
$584.94
Quote
Quote
Reaction
Sample
ADTOP
ADGED
$25.00
$500.00
$25.00
$500.00
$59.69
$1,193.75
Yields are not guaranteed and
vary between individual preps
Lot
ADHPL
$1,908.00
$1,908.00
$4,555.35
Yields are not guaranteed and
vary between individual preps
Lot
ADHPM
$1,428.00
$1,428.00
$3,409.35
Yields are not guaranteed and
vary between individual preps
Lot
ADMPM
$1,020.00
$1,020.00
$2,435.25
Yields are not guaranteed and
vary between individual preps
Lot
ADLPT
$521.00
$521.00
$1,243.89
Yields are not guaranteed and
vary between individual preps
Lot
ADLPL
$180.00
$180.00
$429.75
Yields are not guaranteed and
vary between individual preps
Lot
ADHLR
$1,508.00
$1,508.00
$3,600.35
Yields are not guaranteed and
vary between individual preps
Lot
ADHMR
$1,020.00
$1,020.00
$2,435.25
Lot
ADMMR
$564.00
$564.00
$1,346.55
MicroLiter
ADPS
$1.00
$1.00
$2.39
AAV VECTOR, PER MICROLITER (i.e. individual aliquot requests from existing virus preparations
stocked at the MVSC)
AAV NEUTRALIZING ANTIBODY TITER ASSAY (full serum dilution series for neutralizing antibody titer
determination)
AAV NEUTRALIZING ANTIBODY SCREEN (limited serum dilution testing for neutralizing antibody
screening)
AAV ITR PLASMID GROWTH AND ENDO-FREE MEGA PREP (incl. ITR verification by restriction
enzyme digest)
AAV INFECTIOUS TITER
AAV Endotoxin Test
Adenovirus (AD):
Ad pENTR/D-TOPO plasmid and reagents, per reaction
Ad GATEWAY ENTR TO DEST VECTOR RECOMBINATION
ADENOVIRUS VECTOR HIGH-PURITY LARGE-SCALE PRODUCTION FROM TRANSFECTION (6,000
cm 2 culture scale, 1012 to 1013 particles estimated yield; cesium chloride purified stock suitable for in vivo
experiments, incl. transfection, seed stock generation, physical and infectious particle titration)
ADENOVIRUS VECTOR HIGH-PURITY MEDIUM-SCALE PRODUCTION FROM TRANSFECTION
(2,000 cm 2 culture scale, 1011 to 1012 particles estimated yield; cesium chloride purified stock suitable for
in vivo experiments, incl. transfection, passaging, seed stock generation, production, double cesium
chloride purification, physical and infectious particle titration)
ADENOVIRUS VECTOR MEDIUM-PURITY MEDIUM-SCALE PRODUCTION FROM TRANSFECTION
(2,000 cm 2 culture scale, 1011 to 1012 particles estimated yield, sorbitol-cushion purified stock suitable for
in vitro experiments, incl. transfection, passaging, seed stock generation, production, sorbitol cushion
purification, infectious particle titration)
ADENOVIRUS VECTOR LOW PURITY SEED-STOCK PRODUCTION FROM TRANSFECTION (Crude
Lysate made by transfection, passaging and expansion, suitable for production or some in vitro
applications; titration not included)
ADENOVIRUS VECTOR LOW PURITY SEED-STOCK PRODUCTION FROM LYSATE (Crude Lysate
made by passaging existing virus lysate, suitable for production or some in vitro applications; titration not
included)
(
g
6,000 cm 2 culture scale, 1011 to 1012 particles estimated yield, cesium chloride purified stock suitable for
in vivo experiments, incl. production, double cesium chloride purifcation, physical and infectious particle
titration)
(
g
2
11
12
2,000 cm culture scale, 10 to 10 particles estimated yield, cesium chloride purified stock suitable for
in vivo experiments, incl. production, double cesium chloride purifcation, physical and infectious particle
titration)
ADENOVIRUS VECTOR MEDIUM PURITY MEDIUM-SCALE RE-PRODUCTION (from existing seed
stock; 2,000 cm 2 culture scale, 1011 to 1012 particles estimated yield, sorbitol-cushion purified stock
suitable for in vitro experiments, incl. production, infectious particle titration)
ADENOVIRUS VECTOR, PER MICROLITER (i.e. individual aliquot requests from existing virus
preparations stocked at the MVSC)
Yields are not guaranteed and
vary between individual preps
ADENOVIRUS REPLICATION-COMPETENT VIRUS (RCV) ASSAY (unless animals are housed at
ABSL-2, this is required by IACUC before initiating in vivo studies at OHSU)
Sample
ARCV
$277.00
$277.00
$661.34
ADENOVIRUS PFU OR TCID50 TITER (on 293A cells)
ADENOVIRUS ANTIGEN PREP
Sample
Lot
ATCID
AAGPR
$273.00
Quote
$273.00
Quote
$651.79
Quote
Sample
SIVPP
$13.30
$13.30
$31.75
Sample
SPQPR
$18.10
$18.10
$43.21
Sample
Sample
Sample
SIVCP
SDPR
SRCPR
$13.90
$12.30
$28.90
$13.90
$12.30
$28.90
$33.19
$29.37
$69.00
Lentivirus (HIV, SIV, SHIV):
SIV PLASMA PROCESSING (manual or automated methods)
SIV PLASMA VIRAL LOAD QPCR ASSAY
SIV CAVL PROCESSING (automated method)
SIV STANDARD DNA QPCR ASSAY
SIV STANDARD CELL ASSOC. RNA QPCR ASSAY
Equivalent to DRCA
service
SIV RNA ULTRASENSITIVE NESTED RT-QPCR ASSAY (10 replicate digital PCR)
Sample
SRNPR
$85.00
$85.00
$202.94
SIV DNA ULTRASENSITIVE NESTED QPCR ASSAY (10 replicate digital PCR)
SIV VIRUS STOCK PRODUCTION (SIV from plasmid, one T-150 flask, incl. sMAGI and p27 ELISA)
Sample
Lot
SDNPR
SIVPS
$41.30
Quote
$41.30
Quote
$98.60
Quote
SIV TCID50 TITER (on CEMx174 cells, including p27 ELISA)
SIV TZM-BL OR SMAGI TITER
Sample
Sample
STCID
STMBL
$1,098.00
$274.00
$1,098.00
$274.00
$2,621.48
$654.18
Oregon National Primate Research Center
Molecular Virology Support Core (MVSC) Rates 2015-2016
For reduced F&A, enter amount of F&A in field for calculations:
Comments
47%
Unit
Item Code
Federal
Reduced F&A
Industry
SIV p27 gag ANTIGEN CAPTURE ELISA
SIV ANTIBODY ELISA (Half-MaxTiter)
SIV ANTIBODY ELISA (Single Dilution)
SIV IN VITRO SUSCEPTIBILITY, p27 ELISA
COCULTURE OF ANIMAL TISSUES\Fluid (SIV), including p27 ELISA
SIV, STRAIN mac239 (+/- Δnef), VIRUS STOCK
Sample
Sample
Sample
Sample
Sample
Vial
SP27E
SAELT
SAELS
SIVSE
SCOCX
SDNEF
$69.50
$34.80
$19.80
Quote
Quote
Quote
$69.50
$34.80
$19.80
Quote
Quote
Quote
$165.93
$83.09
$47.27
Quote
Quote
Quote
Cytomegalovirus (CMV):
RhCMV VIRAL LOAD ASSAY (QPCR)
CMV DNA ULTRASENSITIVE NESTED QPCR ASSAY (10 replicate digital PCR)
CMV COCULTURE OF ANIMAL TISSUES (Buccal Swab)
CMV COCULTURE OF ANIMAL TISSUES (Urine)
Sample
Sample
Sample
Sample
CQPCR
CDNPR
COCXB
COCXU
$13.60
$41.30
$50.30
$31.00
$13.60
$41.30
$50.30
$31.00
$32.47
$98.60
$120.09
$74.01
CSS
$354.00
$354.00
$845.18
Service
CMV VIRUS/VECTOR SEED STOCK PRODUCTION (crude lysate from one T-150 flask)
Yields are not guaranteed and
vary between individual preps
Lot
7
9
CMV VIRUS/VECTOR MEDIUM-SCALE PRODUCTION (10 to 10 pfu estimated yield; sorbitol-cushion
purified stock suitable for in vitro and vivo experiments; incl. production and TCID50 assay titer)
Yields are not guaranteed and
vary between individual preps
Lot
CPS
$1,492.00
$1,492.00
$3,562.15
CMV VIRUS/VECTOR MEDIUM-SCALE PRODUCTION (without TCID50 assay titer)
Yields are not guaranteed and
vary between individual preps
Lot
CPSNT
$1,234.00
$1,234.00
$2,946.18
Yields are not guaranteed and
vary between individual preps
Lot
CPSH
$1,345.00
$1,345.00
$3,211.19
MicroLiter
Vial
Lot
CMVPS
CMV68
CATPR
$1.00
$143.00
Quote
$1.00
$143.00
Quote
$2.39
$341.41
Quote
Sample
Sample
Sample
Sample
Sample
CTCID
CPLQP
CGRWT
CAELT
CAELS
$273.00
Quote
Quote
$34.80
$19.80
$273.00
Quote
Quote
$34.80
$19.80
$651.79
Quote
Quote
$83.09
$47.27
Lot
Lot
Lot
CAGPR
RAGPR
SAGPR
Quote
Quote
Quote
Quote
Quote
Quote
Quote
Quote
Quote
Vial
Vial
Vial
Sample
PRFIB
TRF
293A
PRCCX
Quote
$30.00
$30.00
Quote
Quote
$30.00
$30.00
Quote
Quote
$71.63
$71.63
Quote
6
8
CMV VIRUS/VECTOR HALF-MEDIUM-SCALE PRODUCTION (5 x 10 to 5 x 10 pfu estimated yield;
sorbitol-cushion purified stock suitable for in vitro and vivo experiments; incl. production and TCID50
assay titer)
CMV VIRUS/VECTOR, PER MICROLITER (i.e. individual aliquot requests from existing virus
preparations stocked at the MVSC)
RhCMV WT, STRAIN 68-1, PURIFIED STOCK (Stock Prep 5/17/07, P.9, 1.6 x 108 pfu/ml, 0.25 ml/vial;
CMV ANTIGEN PREP
CMV TCID50 (or plaque) TITER (on primary fibroblasts)
CMV PLAQUE PURIFICATION
CMV GROWTH CURVE
RhCMV ANTIBODY ELISA (Half-MaxTiter)
RhCMV ANTIBODY ELISA (Single Dilution)
Expanded Simian Pathogen-Free (ESPF) Agents:
RHESUS CYTOMEGALOVIRUS (RhCMV), CRUDE VIRAL ANTIGEN PREP FOR ESPF SCREENING
RHESUS RHADINOVIRUS
( (RRV), CRUDE VIRAL
g gANTIGEN
p PREP
), FOR ESPF SCREENING
SCREENING
Cells:
PRIMARY RHESUS FIBROBLASTS, LOW PASSAGE (support RhCMV replication)
TELOMERIZED RHESUS FIBROBLASTS (TRF)
293A
PRIMARY CELL CULTURE
Primary cells in limited
availability
Miscellaneous:
DNA ISOLATION, CELL-FREE VIRUS, AUTOMATED (incl. DNA sample spec and dilution)
Sample
DRCF
$13.10
$13.10
$31.28
DNA ISOLATION, CELL-ASSOCIATED VIRUS, AUTOMATED (incl. DNA sample spec and dilution)
SMALL TISSUE PROCESSING (DNA AND RNA) FOR ULTRASENSITIVE VIRUS DETECTION (incl.
DNA/RNA sample spec and dilution)
Sample
DRCA
$13.90
$13.90
$33.19
Sample
DRSTP
$33.30
$33.30
$79.50
LARGE TISSUE PROCESSING (DNA AND RNA) FOR ULTRASENSITIVE VIRUS DETECTION, (incl.
DNA/RNA sample spec and dilution)
Sample
DRLTP
$45.70
$45.70
$109.11
MYCOPLASMA TESTING (by enzymatic or PCR-based detection methods)
DNA ISOLATION (Gentra Kit)
RNA ISOLATION (Qiagen Rneasy Kit)
Sample
Sample
Sample
MYCOT
DGEN
RQIA
$19.00
Quote
Quote
$19.00
Quote
Quote
$45.36
Quote
Quote
WESTERN BLOT (Verification of Transgene Expression)
Sample
WEST
Quote
Quote
Quote
Hour
COND
$50.00
$50.00
$119.38
Consulting Fees:
CONSULTING SESSION (with MVSC Director, hourly rate)