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Exam 1 samples The following choices are used for questions 1 – 5. (a) Redi (d) Pasteur (b) Tyndall (e) Aristotle (c) Leeuanhoek 1. He suggested that mice could develop from spoiled grain by spontaneous generation. 2. He performed experiments using media in “swan necked flasks” that permitted free exchange of air while preventing contaminating particles from entering the flask. In doing so, he countered the objections of Needham and demonstrated that microbes do not develop by spontaneous generation. 3. Using a single-lens microscope, he discovered the existence of microbes. 4. By filtering air before it entered a culture vessel containing media, he demonstrated that airborne microbial contamination came from microbes attached to dust particles in the air. 5. He demonstrated that maggots (fly larvae) developed from fly eggs, not spontaneous generation. The following choices are used for questions 6 – 10. (a) Pasteur (d) Snow (b) Koch (e) Semmelweis (c) Lister 6. He suggested that the incidence of infections in mothers after childbirth could be reduced if the attendants washed their hands before delivering the baby. 7. He developed antiseptic surgical procedures that used carbolic acid (phenol) as a disinfectant. 8. By using maps to localize the source of a cholera epidemic in London to a specific public water pump, he was able to reduce its spread by implementing public health measures. 9. He developed a vaccine for rabies. 10. Using colony isolation techniques on agar media, he isolated the anthrax and tuberculosis bacteria and demonstrated their roles in disease. 13. Microorganisms can be found in the followings EXCEPT A. Air B. Soil C. Lakes D. Sterile media E. None of the above 14. …………..is the concept that living organisms arise from non-living material is called A. Biogenesis. B. cell theory. C. spontaneous generation. D. germ theory. 15. In …….Alexander Flemming discovered that fungus Penicillium produced an antibiotic penicillin. A. 1929 B. 1939 C. 1949 D. 1959 16. ………………used sealed glass flasks containing water and seeds to improve on Needham’s experimental design in trying to settle the conflict over spontaneous generation. A. Reddi B. Koch C. Spallanzani D. Pasteur E. None of the above 17. Who is linked to Bacillus anthracis and anthrax A. Pasteur B. Koch C. Lister D. Reddi 18. Gram-positive bacteria such as S. aureus stain pink A. True B. False 19. In Pasteur’s swan neck flask, A. The neck is for blocking air B. The open end is for introducing microorganisms from air C. The boiled beef broth forms life D. Two of the above E. None of the above 20. Which of the following is NOT a Koch’s Postulate? A. The pathogen should be present in all cases of the disease. B. The pathogen should be absent in healthy individual. C. The pathogen should be isolated in pure culture. D. The pathogen from pure culture should not cause any disease to healthy individual. E. None of the above ***************************Good Luck*************************