Download NEC313N, ACETYL COENZYME A, [ACETYL-1- C]

NEC313N, ACETYL COENZYME A, [ACETYL-1- 14C](Acetyl CoA, [ACETYL-1- 14C]-)
NH2
N
N
O
Lot Number:
Specific Activity:
3631093
_____________________
60
_____________
mCi/mmol
H3C
14
OH
O
N
H
N
H
OH
O
29-Jan-10
_____________
OH
O
O
P
P
O
O
HO
O
HO
P
O
M.W. 809.6
C23H38N7O17P3S
CAS 72-89-9
PACKAGING: Freeze dried solid under nitrogen. Shipped in dry ice.
STABILITY AND STORAGE RECOMMENDATIONS:
When acetyl coenzyme A, [acetyl-1- 14C]- is stored at -20oC as a solid under nitrogen, the rate of
decomposition is initially 3-4 % per year from date of purification. The rate of decomposition
can accelerate. Stability is nonlinear and not correlated to isotope half-life. Lot to lot variation
may occur.
To maintain product purity, this sample should be slowly thawed at 4oC. Repeated freezing and
thawing should be avoided
SPECIFIC ACTIVITY RANGE: 50-62 mCi/mmol (1.85-2.30 GBq/mmol)
RADIOCHEMICAL PURITY: This product was initially found to be greater than 95% when
determined by the following method. It is advisable to check purity prior to use.
1.
High pressure liquid chromatography on a C8 or C18 column using the following mobile
phase:
50mM potassium phosphate buffer, pH 5.3 : MeOH, (85:15).
2.
Paper chromatography on Whatman P-81 using the following solvent system:
n-butanol : acetic acid : water, (5:2:3).
CHEMICAL PURITY: Determined by ultraviolet spectrophotometry in 0.1M potassium
phosphate buffer, pH 7.0. The values obtained for ratios of absorbency and λmax are within the
accepted ranges.
QUALITY CONTROL: The radiochemical purity of acetyl coenzyme A, [acetyl-1- 14C]- is
checked at appropriate intervals using the above chromatography method.
PREPARATIVE PROCEDURE:
N
N
CH3
S
C
2.22
_____________
GBq/mmol
Production Date:
O HC
3
Acetyl coenzyme A, [acetyl-1-14C]- is prepared by the
O
OH
reaction of thiophenylacetate, [acetate-1-14C]- and coenzyme A (2). The product is purified by
ion-exchange chromatography.
HAZARD INFORMATION:
WARNING: This product contains a chemical known to the state of California to cause cancer.