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1 Pox Viruses Hugh B. Fackrell [email protected] poxvirus.ppt 4/29/2017 2 Pox Viruses Structure Classification Multiplication Clinical manifestations Epidemiology Diagnosis Control Baron’s Web Site 4/29/2017 3 Structure Brick shaped complex symmetry 240nm x 300 nm Enveloped antibody neutralization sites Core contains enzymes for virus uncoating dsDNA Inner membrane Lateral bodies 4/29/2017 4 Smallpox virus negatively stained Thin section 4/29/2017 5 Pox Viruses Canary pox virus Vaccinia Orf virus 4/29/2017 6 Pox Viruses of Vertebrates Orthopoxvirus smallpox, vaccinia, cowpox, camelpox, mousepox, racoonpox Parapoxvirus ORF( contagious pustuular dermatitis, pseudocowpox, bovine papular stomatitis Capripoxvirus sheeppox, goatpox, lumpy skin disease 4/29/2017 7 More Pox Viruses Avipoxvirus Fowlpox canarypox,pigeonpox, fowlpox Leporipoxvirus myxoma, hare fibroma, rabbit fibroma,squirrel fibroma Suipoxvirus Swinepox Unclassified molluscum contagiosum, Tana, Yaba 4/29/2017 8 Multiplication Attachment outer membrane of infectious virion fuses with cell membrane Penetration outer membrane remains with cell membrane Uncoating yields infectious intracellular virions via cellular enzymes exposes core proteins DNA dependent RNA polymerase 4/29/2017 9 Clinical manifestations Primary localized skin lesion e.g. cowpox usually on hand occasionally on face or genitals by scratches Generalized skin lesions e.g. small pox, molluscum Encephalitis is rare 4/29/2017 10 Smallpox pustules 4/29/2017 11 Burn infected with vaccinia 4/29/2017 12 Vaccinia complications 4/29/2017 13 Host Defenses unbroken skin represents primary control Interferon, nonspecific inflammation Cell mediated immunity Humoral immunity- outer membrane antigens 4/29/2017 14 Diagnosis Clinical manifestations History of animal contact Electron microscopy of scab material Cell cultures Chicken embryos 4/29/2017 15 Vaccina Cytopathic Effect Human amnion cell line 4/29/2017 16 Chick Embryo Lesions Infected egg Healthy egg 4/29/2017 17 Vaccinia lesions on Chorio allantoic membrane 4/29/2017 18 Cytopathic Effect of Vaccinia Early Cytopathic effect Hep 2 cell line Late Cytopathic Effect 4/29/2017 19 Smallpox was global 4/29/2017 20 4/29/2017 21 Human Pox Viruses are acquired from animal reservoirs 4/29/2017 22 Control infection by zoonotic pox viruses is inevitable improved health education break link with reservoir improved hygiene molluscum contagiosum 4/29/2017 23 4/29/2017 24 Eradication of Smallpox easily diagnosed effective vaccine- only one serotype long incubation period low communicability no long term immunity animal reservoir high morbidity & mortality 4/29/2017 25 Smallpox Eradication (cont) infectious only after incubation period no persistent infection subclinical infection rare and not a source of infections no cultural and social barriers feasbility of elmination shown in developing countries save surveillence costs in nonendemic developed countries 4/29/2017 26 Punch 1802 smallpox vaccine 4/29/2017 27 DONE!!! 4/29/2017
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