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Cell Cycle • The dividing and non-dividing stages in the life of a cell. • Phases: 1. Interphase: Interphase growth and DNA replication 2. Prophase 3. Metaphase Mitotic division 4. Anaphase 5. Telophase Cell Cycle S phase G1 interphase Mitosis -prophase -metaphase -anaphase -telophase G2 Interphase • Comprises about 90% of the cell cycle. cycle • Cellular growth: growth a. protein synthesis b. metabolic activities c. DNA synthesis • Made up of three phases: phases 1. 2. 3. G1 phase S phase G2 phase Interphase 1. G1 (gap) phase: a. protein synthesis and metabolic activities. b. most cell are arrested in this phase. phase 2. S phase: a. DNA replication takes place. 3. G2 (gap) phase: a. Cellular growth and preparation for M phase. phase •Mitosis: •Eukaryotic cells (somatic cells) (asexual reproduction) •DNA duplicates -> 2 sister chromatids •Chromosomes attach to spindle and separate •Used for growth, repair and reproduction (in singlecell organisms) •Makes 2 identical cells Binary Fission • Bacteria, cyanobacteria, and most single celled organisms reproduce by binary fission. fission bacteria chromosome plasma membrane Asexual Reproduction Prophase • Longest phase • Nucleolus disappears • Chromatin material condenses into chromosomes, chromosomes consisting of two sister chromatids. chromatids • Centrioles move apart (not found in plants). • Spindle fibers form and attach from centrioles to centromeres. • Nuclear envelope fragments and disappears Prophase early prophase late prophase centrioles spindle fibers aster fibers nuclear envelope disappearing centromere aster fibers centromere centriole spindle fiber sister chromatid kinetochores sister chromatid Metaphase • Shortest phase • Centrioles are at opposite ends of the cell and attached with aster fibers. fibers • Chromosomes move to the metaphase plate (equatorial plate - center of cell). Metaphase centrioles aster fibers spindle fibers metaphase plate Metaphase spindle of a culture cell Anaphase • Centromeres uncouple and spindle fibers shorten. • Sister chromatids separate and move apart. • After separation, chromatids are now considered chromosomes. chromosomes • During this phase, the cell contains twice the normal number of chromosomes. chromosomes • Cell begins to elongate. elongate • At the end, there are equal numbers of chromosomes at the poles. Anaphase No longer sister chromatids, chromatids now chromosomes aster fibers spindle fibers centrioles Telophase • Cleavage furrow develops in animal cells (Cytokinesis begins). • Cell plate develops in plants (no cleavage furrows in plants). • Nucleolus reappears. • Nuclear membrane reappears. • Chromosomes uncoil. • In the end, two genetically identical nuclei (karyokinesis completed) are present. cell plate Hela cell Intracellular Organization of Microtubules Figure 11.43 Structure of Centrosomes (Part 1) Figure 11.43 Structure of Centrosomes (Part 2) Structure of a Centriole Formation of the Mitotic Spindle (Part 1) Formation of the Mitotic Spindle