Download Genetic Engineering

GENETIC
ENGINEERING
Review Game
Best Arnold character
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DNA that is from two or more organisms is
called…
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2.
3.
4.
Combinant DNA
Remade DNA
Recombinant DNA
Multiple DNA
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Fastest Responders (in seconds)
4.91
KAYLA COCKRELL
5.69
6.09
7
7.6
ETHAN WHITE
NORA WAARANIEMI
EMILY TURNQUIST
MICHAEL GIBBS
Today’s Topics
Genetic Engineering
GMOs and Cloning
Gel Electrophoresis
Stem Cells
Final Results
Genetic Engineering
Which of these is not an example of a vector
used in genetic engineering?
1.
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3.
4.
Plasmid
Yeast
Virus
Bacteria
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What is used to cut DNA?
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Restriction Enzymes
DNA Ligase
Scissors
Recombinant Enzymes
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If I wanted to produce human insulin using a
transformed bacteria, what would I need to do
first?
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Recombine the DNA with a
vector
Cut out the gene of interest
Isolate the gene of interest
Insert the gene into the
bacteria
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I have now made my Recombinant DNA. What
must take place before I can clone the gene?
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4.
Transform the bacteria
Select and screen
Gene cloning
Use DNA Ligase
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DNA fragments cut with EcoRI can be
combined with DNA cut with
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BamHI
HindIII
EcoRI
All of the above
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2&3
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Restriction fragments cut with a restriction
enzyme have a single- stranded 4 base tail of
AATT. What is this called?
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3.
4.
Blunt end
Sticky end
Restriction tail
Ligase
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How does the gene get cloned once it has
been taken in by the bacteria?
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4.
Using PCR
Do more transformations
Bacterial reproduction
Gel electrophoresis
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Once I have selected and screened the
bacteria, I can now …
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Extract the gene
Extract the protein
Give the bacteria to an ill
person
All of these
1&2
1&3
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Fastest Responders (in seconds)
11.89
MICHAEL GIBBS
15.57
KATHERINE
SPURLOCK
THOMAS NETTLETON
SAMUEL KAUTZKY
18.42
32.7
Team Scores
63.33
Terminator
53.75
51.1
50
Kindergarten Cop
Mr. Freeze
Predator
GMOs and Cloning
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Which is easier to clone?
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Animals
Plants
Humans
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Which of these is not a reason for making
crops GMOs?
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20%
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Better Taste
Drought Resistance
Pest Resistant
All of the above
Only 2 and 3
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5
If I wanted to clone a sheep 1, using an egg
from sheep 2, and having sheep 3 deliver the
baby, where do I get the DNA from?
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2.
3.
Sheep 1
Sheep 2
Sheep 3
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The method of cloning described in the last
slide is called?
25%
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2.
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4.
25%
25%
2
3
25%
Nuclear replacement
Somatic cell nuclear
transplantation
Artificial twinning
Egg cell fertilization
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4
Which type of cell can I get the DNA from?
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Heart cell
Skin cell
Liver cell
Muscle cell
None of these
All of these
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Fastest Responders (in seconds)
10.77
NORA WAARANIEMI
11.11
12.1
BRANDON LINGEN
KATHERINE
SPURLOCK
KYLIE JOHNSON
12.79
19.38
LIESE PETERSON
Team Scores
88.2
Terminator
78.65
77.52
75.77
Mr. Freeze
Kindergarten Cop
Predator
Gel Electrophoresis
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Gel Electrophoresis does what?
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4.
DNA Fingerprinting
Paternity testing
Separates molecules by size
Uses electricity
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Since everyone on the planet has different
DNA, identical twins excluded,
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2.
3.
No one has the same DNA
fingerprint
Most people have a
different DNA fingerprint
You are less likely to have
the same DNA fingerprint
as another
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In RFLP analysis, with what do we mix our
DNA samples before running them through a
gel
25%
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2.
3.
4.
25%
25%
2
3
25%
DNA ligase
plasmids
Restriction enzymes
DNA polymerase
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Who is the baby’s father?
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2.
3.
Bob
Larry
Neither
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Which direction would DNA move while being
separated in gel electrophoresis?
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2.
3.
Towards the positive
Towards the negative
Both directions depending
on the person
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Which DNA Fragment is shorter?
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2.
A
B
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Who matches the blood stain?
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2.
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4.
Bob
Sue
John
Lisa
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PCRs main function is to
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4.
Exponentially Amplify DNA
Make a few copies of DNA
Catch criminals
Extract DNA
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Fastest Responders (in seconds)
3.13
MICHAEL GIBBS
5.69
6.01
8.33
8.58
ERIN LOUWAGIE
KEVIN STOWE
NORA WAARANIEMI
CALEB TROUTH
Team Scores
117.94
Predator
117.78
110.98
102.19
Terminator
Mr. Freeze
Kindergarten Cop
Stem Cells
Which of these can differentiate into the
widest variety of cells?
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2.
3.
Totipotent
Multipotent
Pluripotent
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Embryonic stem cells are
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Totipotent
Pluripotent
Multipotent
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Where do most adult stem cells used in
medicine come from?
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4.
Kidneys
Skin
Blood
Bone marrow
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Adult stem cells are
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2.
3.
Totipotent
Pluripotent
Multipotent
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Which type of stem cell is created from a
differentiated somatic cell?
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2.
3.
Adult stem cells
Embryonic stem cells
Induced pluripotent stem
cells
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Fastest Responders (in seconds)
4.87
MICHAEL GIBBS
Team Scores
142.76
Mr. Freeze
137.94
137.78
132.19
Predator
Terminator
Kindergarten Cop
Team MVP
Points
Team
Participant
179.91
147.07
151.84
163.27
Mr. Freeze
Predator
Terminator
Kindergarten Cop
MICHAEL GIBBS
ERIN LOUWAGIE
TIFFANY LO
EMILY TURNQUIST
Participant Scores
179.91
MICHAEL GIBBS
129.4
TRISHA MORRISON
165.66
163.27
151.84
150.67
BRANDON LINGEN
EMILY TURNQUIST
TIFFANY LO
CALEB TROUTH
126.43
120.2
118.49
118.43
TAYLOR HAEFELE
RISHA SINGH
KAYLA COCKRELL
KYLIE JOHNSON
147.07
141.37
ERIN LOUWAGIE
KATHERINE
SPURLOCK
115.6
112.91
PETER KILIAN
PETER FOMIN
135.43
132.76
132.11
LIESE PETERSON
NORA WAARANIEMI
ETHAN WHITE
112.38
103.77
101.31
KEVIN STOWE
THOMAS NETTLETON
LYDIA BOIKE