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Supplemental Table S1 Analysis of variance for the contents of seed protein, oil, amino acid and fatty acids of soybean cultivars DF 3 85 DF Region 3 Cultivar(Region) 85 Region Cultivar(Region) Protein 387.38**† 6.14** His 0.48** 0.04** Oil 199.43** 2.64** Thr 5.12** 0.43** PA 5.16** 1.20** Arg 0.42** 0.05** SA 9.99** 0.40** Ala 1.14** 0.08** OA 337.90** 23.16** Tyr 0.70** 0.06** LA 135.09** 14.77** Val 1.03** 0.05** LNA 21.67** 0.97** Phe 1.60** 0.12** Asp 1.91** 0.20** Leu 0.11** 0.05** Glu 19.19** 0.49** Ile 1.42** 0.18** Ser 3.55** 0.16** Lys 1.97** 0.34** Gly 0.03** 0.01** Pro 17.18** 0.70** ** represent the significance levels at P≤0.01. DF, Degrees of freedom † PA, Palmitic acid; SA, Stearic acid; OA, Oleic acid; LA, Linoleic acid; LNA, Linolenic acid; Asp, Aspartic acid; Glu, Glutamic acid; Ser, Serine; Gly, Glycine; His, Histidine; Thr, Threonine; Arg, Arganine; Ala, Alanine; Tyr, tyrosine; Val, Valine; Phe, Phenylalanine; Leu, Leucine; Ile, Isoleucine; Lys, Lysine; Pro, Proline Supplemental Table S2 Statistics of seed protein and oil composition of widely grown soybean cultivars in different regions region Protein Oil PA SA OA LA LNA Asp Glu Ser Gly His Thr Arg Ala Tyr Val Phe Leu Ile Lys Pro HH region JL region MSH region NH region Min. Max. Mean STD Min. Max. Mean STD Min. Max. Mean STD Min. Max. Mean STD 40.99 47.31 44.28 1.69 34.86 40.25 37.46 1.38 37.12 44.07 41.03 1.59 39.45 46.19 42.08 2.32 15.39 20.68 17.50 1.46 20.41 24.07 22.30 1.18 18.55 22.17 20.12 0.83 16.87 20.11 18.72 0.86 11.05 13.57 12.24 0.64 10.42 13.23 11.58 0.71 10.62 13.70 11.83 1.00 10.18 12.64 11.55 0.67 3.60 4.99 4.38 0.34 3.16 4.71 3.98 0.35 3.62 5.81 4.90 0.60 4.31 5.77 5.04 0.46 19.97 29.39 23.77 2.59 23.48 40.38 29.52 4.19 20.70 34.80 27.31 3.69 19.14 28.25 24.11 2.62 47.79 56.32 51.82 2.13 40.24 53.15 48.40 3.34 41.46 53.40 48.68 2.88 47.40 54.38 51.13 2.11 6.39 9.70 7.79 0.90 5.29 7.68 6.52 0.58 6.48 8.49 7.29 0.59 7.23 9.45 8.17 0.60 3.06 5.28 4.82 0.48 3.50 4.76 4.34 0.25 4.26 4.88 4.63 0.16 4.36 5.32 4.72 0.26 4.10 7.58 6.58 0.68 4.40 5.96 5.12 0.46 5.20 6.46 6.13 0.34 5.72 7.10 6.34 0.36 1.81 3.48 2.61 0.29 1.77 2.65 2.41 0.22 2.41 3.61 3.06 0.37 2.12 2.96 2.64 0.21 0.71 0.87 0.79 0.04 0.64 0.91 0.77 0.06 0.66 0.92 0.83 0.06 0.55 0.93 0.78 0.08 0.67 1.84 0.99 0.20 0.61 1.00 0.80 0.11 0.92 1.18 1.01 0.08 0.84 1.18 1.02 0.07 2.73 5.26 3.96 0.67 2.35 3.83 3.24 0.36 3.04 4.54 3.56 0.37 3.26 4.36 3.95 0.31 1.16 1.83 1.56 0.15 1.06 1.69 1.33 0.15 0.99 1.63 1.44 0.14 1.05 1.95 1.47 0.21 2.16 3.15 2.91 0.21 2.02 3.06 2.66 0.26 2.29 3.09 2.95 0.17 2.87 3.43 3.03 0.14 1.42 1.99 1.78 0.14 1.14 1.94 1.62 0.15 1.77 2.10 1.92 0.10 0.66 2.02 1.77 0.29 1.47 2.43 2.19 0.18 1.47 2.14 1.94 0.17 1.98 2.64 2.28 0.17 1.96 2.42 2.22 0.11 1.50 2.90 2.24 0.26 1.05 2.46 1.88 0.36 2.05 2.58 2.26 0.15 2.05 2.54 2.28 0.15 1.27 2.27 2.10 0.20 1.59 2.28 2.04 0.19 1.72 2.27 2.16 0.11 1.95 2.24 2.14 0.07 2.89 3.76 3.43 0.25 2.40 3.48 3.16 0.26 3.00 4.02 3.52 0.33 2.79 4.43 3.54 0.36 1.74 3.55 2.67 0.40 1.82 3.45 2.53 0.37 2.10 3.67 2.95 0.40 2.06 3.80 2.94 0.47 1.19 4.47 2.53 0.70 0.95 3.31 1.57 0.55 0.43 1.90 1.17 0.37 0.44 3.15 1.71 0.73 PA, Palmitic acid; SA, Stearic acid; OA, Oleic acid; LA, Linoleic acid; LNA, Linolenic acid; Asp, Aspartic acid; Glu, Glutamic acid; Ser, Serine; Gly, Glycine; His, Histidine; Thr, Threonine; Arg, Arganine; Ala, Alanine; Tyr, tyrosine; Val, Valine; Phe, Phenylalanine; Leu, Leucine; Ile, Isoleucine; Lys, Lysine; Pro, Proline. HH, Huang-Huai-Hai River Valley region; JL, Jilin and Liaoning region; MSH, Mid-south Heilongjiang region; and NH, Northern Heilongjiang