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Supplemental Table S1 Analysis of variance for the contents of seed protein, oil, amino acid and fatty acids of soybean cultivars
DF
3
85
DF
Region
3
Cultivar(Region) 85
Region
Cultivar(Region)
Protein
387.38**†
6.14**
His
0.48**
0.04**
Oil
199.43**
2.64**
Thr
5.12**
0.43**
PA
5.16**
1.20**
Arg
0.42**
0.05**
SA
9.99**
0.40**
Ala
1.14**
0.08**
OA
337.90**
23.16**
Tyr
0.70**
0.06**
LA
135.09**
14.77**
Val
1.03**
0.05**
LNA
21.67**
0.97**
Phe
1.60**
0.12**
Asp
1.91**
0.20**
Leu
0.11**
0.05**
Glu
19.19**
0.49**
Ile
1.42**
0.18**
Ser
3.55**
0.16**
Lys
1.97**
0.34**
Gly
0.03**
0.01**
Pro
17.18**
0.70**
** represent the significance levels at P≤0.01.
DF, Degrees of freedom
†
PA, Palmitic acid; SA, Stearic acid; OA, Oleic acid; LA, Linoleic acid; LNA, Linolenic acid; Asp, Aspartic acid; Glu, Glutamic acid; Ser, Serine;
Gly, Glycine; His, Histidine; Thr, Threonine; Arg, Arganine; Ala, Alanine; Tyr, tyrosine; Val, Valine; Phe, Phenylalanine; Leu, Leucine; Ile,
Isoleucine; Lys, Lysine; Pro, Proline
Supplemental Table S2 Statistics of seed protein and oil composition of widely grown soybean cultivars in different regions
region
Protein
Oil
PA
SA
OA
LA
LNA
Asp
Glu
Ser
Gly
His
Thr
Arg
Ala
Tyr
Val
Phe
Leu
Ile
Lys
Pro
HH region
JL region
MSH region
NH region
Min.
Max.
Mean
STD
Min.
Max.
Mean
STD
Min.
Max.
Mean
STD
Min.
Max.
Mean
STD
40.99
47.31
44.28
1.69
34.86
40.25
37.46
1.38
37.12
44.07
41.03
1.59
39.45
46.19
42.08
2.32
15.39
20.68
17.50
1.46
20.41
24.07
22.30
1.18
18.55
22.17
20.12
0.83
16.87
20.11
18.72
0.86
11.05
13.57
12.24
0.64
10.42
13.23
11.58
0.71
10.62
13.70
11.83
1.00
10.18
12.64
11.55
0.67
3.60
4.99
4.38
0.34
3.16
4.71
3.98
0.35
3.62
5.81
4.90
0.60
4.31
5.77
5.04
0.46
19.97
29.39
23.77
2.59
23.48
40.38
29.52
4.19
20.70
34.80
27.31
3.69
19.14
28.25
24.11
2.62
47.79
56.32
51.82
2.13
40.24
53.15
48.40
3.34
41.46
53.40
48.68
2.88
47.40
54.38
51.13
2.11
6.39
9.70
7.79
0.90
5.29
7.68
6.52
0.58
6.48
8.49
7.29
0.59
7.23
9.45
8.17
0.60
3.06
5.28
4.82
0.48
3.50
4.76
4.34
0.25
4.26
4.88
4.63
0.16
4.36
5.32
4.72
0.26
4.10
7.58
6.58
0.68
4.40
5.96
5.12
0.46
5.20
6.46
6.13
0.34
5.72
7.10
6.34
0.36
1.81
3.48
2.61
0.29
1.77
2.65
2.41
0.22
2.41
3.61
3.06
0.37
2.12
2.96
2.64
0.21
0.71
0.87
0.79
0.04
0.64
0.91
0.77
0.06
0.66
0.92
0.83
0.06
0.55
0.93
0.78
0.08
0.67
1.84
0.99
0.20
0.61
1.00
0.80
0.11
0.92
1.18
1.01
0.08
0.84
1.18
1.02
0.07
2.73
5.26
3.96
0.67
2.35
3.83
3.24
0.36
3.04
4.54
3.56
0.37
3.26
4.36
3.95
0.31
1.16
1.83
1.56
0.15
1.06
1.69
1.33
0.15
0.99
1.63
1.44
0.14
1.05
1.95
1.47
0.21
2.16
3.15
2.91
0.21
2.02
3.06
2.66
0.26
2.29
3.09
2.95
0.17
2.87
3.43
3.03
0.14
1.42
1.99
1.78
0.14
1.14
1.94
1.62
0.15
1.77
2.10
1.92
0.10
0.66
2.02
1.77
0.29
1.47
2.43
2.19
0.18
1.47
2.14
1.94
0.17
1.98
2.64
2.28
0.17
1.96
2.42
2.22
0.11
1.50
2.90
2.24
0.26
1.05
2.46
1.88
0.36
2.05
2.58
2.26
0.15
2.05
2.54
2.28
0.15
1.27
2.27
2.10
0.20
1.59
2.28
2.04
0.19
1.72
2.27
2.16
0.11
1.95
2.24
2.14
0.07
2.89
3.76
3.43
0.25
2.40
3.48
3.16
0.26
3.00
4.02
3.52
0.33
2.79
4.43
3.54
0.36
1.74
3.55
2.67
0.40
1.82
3.45
2.53
0.37
2.10
3.67
2.95
0.40
2.06
3.80
2.94
0.47
1.19
4.47
2.53
0.70
0.95
3.31
1.57
0.55
0.43
1.90
1.17
0.37
0.44
3.15
1.71
0.73
PA, Palmitic acid; SA, Stearic acid; OA, Oleic acid; LA, Linoleic acid; LNA, Linolenic acid; Asp, Aspartic acid; Glu, Glutamic acid; Ser, Serine;
Gly, Glycine; His, Histidine; Thr, Threonine; Arg, Arganine; Ala, Alanine; Tyr, tyrosine; Val, Valine; Phe, Phenylalanine; Leu, Leucine; Ile,
Isoleucine; Lys, Lysine; Pro, Proline.
HH, Huang-Huai-Hai River Valley region; JL, Jilin and Liaoning region; MSH, Mid-south Heilongjiang region; and NH, Northern Heilongjiang