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Video 1A. 3D reconstruction of confocal fluorescent image of control eNK cell conjugated to
K562 target cell, as depicted in Figure 2.2A. Actin (blue) and perforin (green) are shown.
Video 1B. 3D reconstruction of confocal fluorescent image of myosin IIA 1933x patient eNK cell
conjugated to K562 target cell, as depicted in Figure 2.2B. Actin (blue) and perforin (green) are
shown.
Video 1C. 3D reconstruction of confocal fluorescent image of blebbistatin-treated control eNK cell
conjugated to K562 target cell, as depicted in Figure 2.2C. Actin (blue) and perforin (green) are
shown.
Video 1D. 3D reconstruction of confocal fluorescent image of control eNK cell conjugated to
K562 target cell, as depicted in Figure 2.2A. Myosin IIA (red), actin (blue), and perforin (green)
are shown.
Video 1E. 3D reconstruction of confocal fluorescent image of myosin IIA 1933x patient eNK cell
conjugated to K562 target cell, as depicted in Figure 2.2B. Myosin IIA (red), actin (blue), and
perforin (green) are shown.
Video 1F. 3D reconstruction of confocal fluorescent image of blebbistatin-treated control eNK cell
conjugated to K562 target cell, as depicted in Figure 2.2C. Myosin IIA (red), actin (blue), and
perforin (green) are shown.
Video 2. Confocal fluorescent time-lapse image of YTS-MyoIIA-GFP cells loaded with
Lysotracker Red and conjugated to KT86 target cells, as described in Figure 2.6D. In top panel,
Lysotracker Red (red) and GFP (green) fluorescence are shown. In bottom panel, a pseudocolor
scale is used to show intensity of GFP (Lysotracker Red fluorescence was removed prior to
applying color scale).
Video 3. Confocal fluorescent time-lapse image of YTS-GFP cells loaded with Lysotracker Red
and conjugated to KT86 target cells, as described in Figure 2.6E. In top panel, Lysotracker Red
(red) and GFP (green) fluorescence are shown. In bottom panel, a pseudocolor scale is used to
show intensity of GFP (Lysotracker Red fluorescence was removed prior to applying color scale).
Video 4A. Confocal fluorescent time-lapse image of lytic granules isolated from YTS-MyoIIA-GFP
cells added to a BSA/SA-coated microflow chamber, as described in Figure 2.13A.
Video 4B. Confocal fluorescent time-lapse image of lytic granules isolated from YTS-MyoIIA-GFP
cells added to a BSA/SA- and actin-coated microflow chamber in physiologic salt buffer, as
described in Figure 2.13A.
Video 4C. Confocal fluorescent time-lapse image of lytic granules isolated from YTS-MyoIIA-GFP
cells added to a BSA/SA- and actin-coated microflow chamber in ATP-containing high-salt buffer,
as described in Figure 2.13A.
Video 4D. Confocal fluorescent time-lapse image of blebbistatin-treated lytic granules isolated
from YTS-MyoIIA-GFP cells added to a BSA/SA- and actin-coated microflow chamber in
physiologic salt buffer, as described in Figure 2.13A.
Video 5A. Confocal fluorescent time-lapse image of Lysotracker Green-loaded lytic granules
isolated from control eNK cells. Granules were added to a BSA/SA- and actin-coated microflow
chamber in physiologic salt buffer, as described in Figure 2.13C.
Video 5B. Confocal fluorescent time-lapse image of Lysotracker Green-loaded lytic granules
isolated from control eNK cells. Granules were added to a BSA/SA- and actin-coated microflow
chamber in ATP-containing high-salt buffer, as described in Figure 2.13C.
Video 6A. Confocal fluorescent time-lapse image of Lysotracker Green-loaded lytic granules
isolated from myosin IIA 1933x eNK cells. Granules were added to a BSA/SA- and actin-coated
microflow chamber in physiologic salt buffer, as described in Figure 2.13D.
Video 6B. Confocal fluorescent time-lapse image of Lysotracker Green-loaded lytic granules
isolated from myosin IIA 1933x eNK cells. Granules were added to a BSA/SA- and actin-coated
microflow chamber in ATP-containing high-salt buffer, as described in Figure 2.13D.
Video 7A. TIRFm time-lapse image of control YTS cell loaded with Lysotracker Green and placed
into anti-CD28-coated environmental chamber, as described in Figure 2.13A.
Video 7B. TIRFm time-lapse image of ML-9-treated YTS cell loaded with Lysotracker Green and
placed into anti-CD28-coated environmental chamber, as described in Figure 2.13A.