Download Monthly Lecture Schedule

BIOCHEMISTRY DEPARTMENT
LECTURE SUMMARY OF THEORY CLASSES FOR THE MONTH OF FEBURARY
1. GENE EXPRESSION DATE: 10-2-16 BY Dr. JITENDRA AHUJA
The lac operon and its control
Gene expression in Eukaryotes
 Chromatin structure and gene expression
 Histone acetylation and deacetylation
 Methylation of DNA and inactivation of gene
 Enhancers and tissue specific gene expression
 Motifs in proteins and Gene expression
 Helix turn helix motif –lac operon
 Zink finger motif
 Leusin zipper motif
 Helix-loop-helix motif
Eukaryote gene expression is regulated at six levels:
1. Transcription
2. RNA processing
3. mRNA transport
4. mRNA translation
5. mRNA degradation
6. Protein degradation
2. GENETIC CODE & TRANSLATION DATE: 11-2-16 BY Dr. SUNIL GUPTA
Characteristics of Genetic code, material required for protein synthesis
3. FLAME PHOTOMETRY AND FLOURIMETRY DATE: 13-2-16 BY Dr.
PRIYANKA JAIN
Introduction, Principle of the instruments, components & design of both instruments
Working procedure of the instrument, uses/ clinical applications of the instruments
Types of flourimetry & factors affecting fluorescence intensity
4. BIOCHEMISTRY OF AIDS DATE: 17-2-16 BY Dr. PRIYANKA JAIN
Epidemics of AIDS & Indian scenario
Mode of transmission, natural course of disease (window period, seropositive stage,
disease), clinical presentation, laboratory analysis of AIDS
Structure, replication, assembly & maturation of HIV virus, immunology of AIDS,
genetic resistance, genetic heterogeneity of virus
Prospects of a vaccine, anti-HIV drugs & prevention of AIDS
5. TRANSLATION CONTD. DATE: 18-2-16 BY Dr. SUNIL GUPTA
Steps of protein synthesis i.e. activation of amino acids, initiation, elongation and
termination.
6. BIOCHEMISTRY OF CANCER DATE: 20-02-2016 BY Dr. MANJU JHA




Introduction
Characteristics of cancer
Benign & Malignant tumour
Etiology of cancer
o Environmental factors/ Chemical carcinogen
o Viruses
o Lifestyle factors
o Medical - Drugs and Hormones
o Hereditary
o Industrial/Occupational
o
Coal miners, factory workers, asbestos
o Ultraviolet Light -  risk of skin cancers
o Ionizing Radiation
o X-rays, nuclear weapons or accidents
o Thyroid irradiation (for hyperthyroid)
o Chemical carcinogens
7. METABOLISM OF XENOBIOTICS DATE: 24-02-2016 BY Dr. SONALI
SHARMA
Detoxification mechanisms,cytochrome P450 enzyme systems,
phases of
detoxification processes , phase one reactions (oxidation,reduction ,hydrolysis),phase
2 reactions ( conjugation with glucuroinc acid,sulphate,methylation)
8. TRANSLATION CONTD. DATE: 25-2-16 BY Dr. SUNIL GUPTA
 Energy requirements in protein synthesis
 Inhibitors of protein synthesis
9. BIOCHEMISTRY OF CANCER CONTD. DATE: 27-02-2016 BY Dr. MANJU
JHA









Oncogenes
DNA Repair genes
Proteins encoded by proto-oncogenes
Mechanism of activation of oncogenes
o Promoter insertion
o Enhancer insertion
o Chromosomal translocation
o Gene amplification
o Point mutation
Growth factors
Oncosupressor genes/ Tumour suppressor genes
Rb 1 gene
p-53 gene
Tumour markers (Common tumour markers)
BIOCHEMISTRY DEPARTMENT
PRACTICAL SUMMARY FOR THE MONTH OF FEBURARY
EXERCISE1. ANALYSIS OF BLOOD- Introduction of blood. Perform physical
examination of blood i.e. appearance, colour, odour, specific gravity & reaction.
Perform tests for the presence of blood by Benzidine & O-Toluidine methods.
EXERCISE2. STUDY OF BILE JUICE- Introduction, composition & functions of
bile. Perform physical examination of bile i.e. volume, appearance, colour, odour,
specific gravity & reaction. Perform tests for the presence of bile salts & bile pigments
by Hay’s surface tension & Petten kopfer’s test and Gmellin & Van den Bergh’s test
respectively.
EXERCISE3. PATHOLOGICAL EXAMINATION OF URINE-Introduction &
composition. Perform physical examination of pathological urine i.e. volume,
appearance, colour, odour, specific gravity (measurement of specific gravity of urine
by using urinometer with temperature correction) & reaction. Perform tests for the
presence of proteins, carbohydrate, ketone bodies, bile salts, bile pigments & blood in
pathological urine.