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Force Microscopy of Nonadherent Cells: A Comparison of Leukemia Cell Deformability by Rosenbluth, Lam & Fletcher Sangjin Ryu Department of Mechanical Engineering Background Better knowledge of mechanical property changes in leukemia cells → Improved understanding of leukemia Atomic force microscopy (AFM) to measure the stiffness of cells → Lab module 2: Elastic modulus Nonadhesive cells tend to slip from the cantilever tip under an applied load How to Fix Leukemia Cells? Sticky surface Trapping within Millipore filters Micropipette aspiration Microfabricated well Rosenbuth, Lam & Fletcher 2006 Biophys. J. Experiments Cells for investigation Acute myeloid leukemia cell: HL60 cell Acute lymphoid leukemia cell: Jurkat cell Normal leukocyte: Human neutrophil Tips for investigation Pyramid AFM tip Sphere indenter Mechanical Models Hertzian model: Elastic modulus 2 4 F 1 2 3E tan Liquid droplet model: Membrane tension 8TS 2 1 cos d k Rc S Results Hertzian model is better Stiffness: HL60>Jurkat>Neutrophil