Download AOI A oryzae inoculum dairy ration

In Vitro Fermentation Characteristic and
Digestibility of Dairy Ration
Supplemented with Aspergillus oryzae
inoculum
• S.I.W Rakhmani,1, Y. Widiawati1 , D.A. Kusumaningrum1 and Salfina N. Ahmad1
• Research Centre of Animal Husbandry, Research Organisastion of Agriculture and Food,
Indonesian National Research and Innovation Agency (BRIN) , Cibinong Science Center, Jl.
Raya Jakarta-Bogor, Cibinong, Kabupaten Bogor 16915
Outline
❑ Introduction
❑Material and Methods
❑ Results and Discussion
❑Conclusion
INTRODUCTION
• Missed use of antibiotics in animal feed industry, using
probiotic for Antibiotic replacement
Feeding probiotic agent →direct-fed microbials
(DFMs) .
Prebiotic feeding such as AOC
Aspergillus oryzae culture (AOC)
❖ regulate rumen microbiota
❖ improve fiber digestibility
❖ lactating cows increased milk production,
❖ feed efficiency,
❖ tolerance to heat stress
A. oryzae is non-pathogenic fungi and generally regarded as safe (GRAS)

The fungus is also known called ‘koji mold’,

Asian foods such as rice wine (sake), soy sauce (shoyu), soybean paste (miso) etc .

The aim:
The effect of A. oryzae inoculum supplementation on
the
• degradation rate of feed rations in vitro
• rumen fermentation parameters such as (NH3, VFA,
CH4)
• crude protein, fibre, dry matter and organic matter
digestibility
Material and Method

Aspergillus oryzae inoculum
production in rice substrate.
 Fungal Cultivation. Single colony of
purification streak into PDA slant
 5-day old spore was then harvested
using 10 mL saline sterile/slant.
 The spore suspension (20 mL
7
suspension / kg substrate; 9.6 × 10
±3456 spores/mL)
 sterile plastic trays size 20x30 cm
aerobically (3 days) RT, dried under
60℃, gound → AOI powder
substrate
Inoculum
spores
In vitro Experiment
• Four treatments:
• Control (C, Commercial dairy feed),
• C2 (Commercial dairy feed + 2 g AOI),
• C4 (Commercial dairy feed + 4 g AOI)
• C8 (Commercial dairy feed + 8 g AOI)
• 500 mg rations incubated at 39℃ using
rumen-buffered mixture for 48 h
• Analysis: TVFA, NH3, Digestibility (CP, NDF,
DM, OM), proximate analysis
Nutrient content Ration
AOI
DM (%)
95,7
92,04
OM (%)
91,43
87,54
CP (%)
14,25
48,36
EE (%)
3,76
1,23
CF (%)
12,4
11,86
NDF (%)
38,04
ADF (%)
21,17
Ash (%)
11,41
1,48
GE (MJ/Kg)
17,28
18,38
Ca
1,28
0,23
P
1,05
0,49
Cellulase (IU/g)
872
TPC (total plate count)/gram
1,98E+08
RESULTS –
PROXIMATE
ANALYSIS
Parameter
pH
C
6.34
TVFA , mmol/L
106.37c 122.42a 132.81a 107.11c 4.890 0.032
Acetate, %
Propionate, %
60.17
21.63
59.00
20.28
58.89
22.04
59.86
22.34
1.357 0.854
0.928 0.797
Butyrate, %
11.90
12.84
12.07
12.16
1.225 0.234
Isobutyrate, %
2.10
2.07
2.01
1.95
0.782 0.969
Valerate, %
Isovalerate, %
2.14
2.05
2.93ab
2.86a
2.26bc
2.32b
2.32c 0.215 0.012
2.71ab 0.127 0.011
NH3-N, mg/dL
13.14
10.19
11.45
12.60
1.128
Acetate/Propionate 2.82
2.69
2.56
2.76
0.350 0.946
MCP, mg/mL
1.55
1.45
1.54
0.104 0.706
1.44
C2
6.14
C4
6.65
C8
6.17
P
SEM value
0.272 0.934
0.463
RESULT→TABLE 2. VFA,
TOTAL VFA AND PH
DURING IN VITRO
FERMENTATION OF FEED
RATION SUPPLEMENTED
WITH AOI
Result→Table 3. CP, NDF, DM and OM Digestibility (%)
Crude
protein
NDF
DMD
OMD
C
C2
C4
C8
45.76 ± 2.288
47.25± 2.363
56.34± 3.482
93.56± 4.675
47.67± 3.886
49.52± 4.002
65.43± 4.982
93.65± 4.768
55.43± 3.241
54.49±3.224
66.75± 4.021
93.78± 5.001
54.50± 3.128
53.24± 4.118
64.57± 4.678
93.86± 4.239
Conclusion→
4 gram of AOI supplementation in ration
increase digestibilities and better rumen performance. Recommend for next
step → animal feeding
Acknowledgments
❖ This research activity is part of the
results of the dairy cattle nutrition
project funded by the Indonesian
Agency for Agricultural Research and
Development – Ministry of
Agriculture research grant no
1806.202.001.051C/K2/APBN/2020.
❖ Authors wishing to acknowledge Ms.
Eka for preparation of inoculum and
Mr. Helmy Hamid for laboratory
measurements.