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Vendor Exhibits Applied Biosystems BD - Biosciences - Industrial Microbiology - Life Sciences — Pre-Analytical Systems Invitrogen Biocision Mettler Toledo BioTek Instruments Millipore Sigma Boekel Scientific Ohaus Corporation Brady Americas Sartorius Stedim Brandtech Scientific Spencer Vernoche/Mott Labs Buchi Corporation Corning, Inc. Draeger Dynalon Eppendorf North America Fisher Scientific -Acros - Alfa Aesar - Fisher BioReagents - Fisher Chemicals -Fisherbrand -Isotemp Ge Healthcare - Life Sciences Consumables - Life Sciences Dharmacon - Life Sciences HyClone Gilson -Consumables -Evos Directions to the Wyndham Pittsburgh University Center 100 Lytton Avenue Pittsburgh PA 15213 Kimberly-Clark FISHER SCIENTIFIC Invites Laboratory Personnel to the 22nd Annual Oakland Scientific Conference KNF Neuberger Spex SamplePrep/CertiPrep Thermo Scientific - Auto Liquid Handling - Cell Culture and Centrifugation -Chromatography Consumables - Cold Storage Equipment - Dionex ICP/GC/HPLC -FT-IR - Laboratory Equipment - Pierce - Real Time PCR Instruments - Molecular Biology -Nalgene/Nunc/MLH/ Orion - Water Purification Systems Vistalab Technologies FROM THE PENNSYLVANIA TURNPIKE • Take I-376 (west) to Exit 7A (Oakland-885N), bearing right onto Bates Street for one-half mile. • Turn left onto Bouquet Street. • Follow Bouquet Street to Forbes Avenue. • Turn right on Forbes Avenue. • Turn left on S. Bellefield Avenue, continue to Fifth Avenue, turn left, then right on Tennyson Avenue. Wednesday, May 4, 2016 9:30 a.m. to 2:00 p.m. FROM I-79 • Exit I-279 Pittsburgh to I-376 Oakland. • Exit Forbes Avenue. • Turn left on S. Bellefield Avenue and follow directions above. FROM THE AIRPORT • Follow signs for I-376 East. • Take Exit 3A (Forbes Avenue/Oakland). • Follow Forbes Avenue to South Bellefield Avenue, make a left. • Turn right on Tennyson Avenue. 100 Lytton Avenue, Pittsburgh, PA 15213 Formerly the Holiday Inn — same location! Next to Alumni Hall S E M I N A R S 22nd Annual Oakland Scientific Conference Wednesday, May 4, 2016 9:30 a.m. to 2:00 p.m. 1.Real-Time PCR Training Seminar Carnegie I Part I: 10:00-10:45 AM Part II: 11:00 – 11:45 AM Speaker: Mike Troutman, Senior Scientific Liaison, Thermo Fisher Scientific The seminar will begin with a basic introduction and tutorial of Real-Time PCR to include terminology, reaction components, amplification, assay design, optimization, reference and control options. Following the introduction, an overview of qPCR-associated applications and solutions will be discussed, to include DNA (mutation detection, single nucleotide polymorphisms/SNPs and high resolution melt/HRM), RNA (gene expression and small RNAs) and protein analysis (protein expression and thermal shift applications). 2. The Science of Western Blotting - Proven Strategies to Optimize Your Western Blots Carnegie II 10:00 – 10:45 AM Formerly the Holiday Inn — same location! Next to Alumni Hall Morning—Coffee, Tea, Pastries, Bagels in Prehall Lunch—Served at Noon in Ballroom Flavored Coffee—Served All Day in Prehall Speaker: Joseph Kramer, Director of Sales, North America, Millipore Sigma Western blotting is a cornerstone of protein research and is used extensively to detect and quantify proteins in complex mixtures. Optimization of reagent concentrations, materials and technique is key to successful blotting. Rethink Western blotting to take protein detection to new dimensions. The SNAP i.d. 2.0 unique vacuum-driven technology promotes antigen binding and thorough washing, enabling you to better optimize your Western blotting conditions - in 30 minutes or less. 40+ Vendor Exhibits Schenley Ballroom No pre-registration required for vendor exhibits Technical Seminars Located in Carnegie I & II – behind Schenley Ballroom PRE-REGISTER online at: www.fishersci.com/oaklandconference 3. The Current Landscape of Biological Testing using High Content Analysis Carnegie II 11:00-11:45 AM 12:45 – 1:30 PM Speaker: Nicholas M. Radio, Ph.D., Technical Sales Specialist, Thermo Fisher Scientific High Content Analysis is an image cytometry-based methodology used to measure cellular and small organism biology. The automated nature of the imaging and quantitative analysis enables the ability to measure numerous timepoints and concentrations that are conventionally difficult to accomplish using manual methods. This technical seminar will provide an overview of applications where the technology is applied, including receptor activation, cellular signaling, viability, morphology and motility phenotypes. 4. Materials and Systems for Optimizing 3D Cell Culture Environments Carnegie I 12:45-1:30 PM Speaker: Mark Rothenberg, Manager Scientific Training and Education, Corning, Inc. Three-dimensional (3D) cell culture environments provide structural and biochemical cues for cellular differentiation and functionality. For specialized cell types such as primary cells and stem cells, a two-dimensional (2D) growth substrate may not be sufficient to support complex cellular behaviors such as cell polarity, morphology, spheroid formation, signal transduction and tissue-specific gene expression. This seminar will highlight applications that rely on the use of 3D growth substrates and specialized culture systems (i.e., permeable supports).The biological and functional properties of a variety of cell types will be discussed to demonstrate the effectiveness of these environments. Seminar pre-registration suggested: www.fishersci.com/oaklandconference Continental Breakfast at 9:30 A.M. — Lunch at Noon No charge for Seminars Questions? Email: 40+ Supplier Exhibits Janice Brophy — [email protected] Please stop by!! or Mary Ann Prignon — [email protected]