Download New Assay: Respiratory Virus Panel by PCR

Excellence in Pathology and Laboratory Services
New Assay: Respiratory Virus Panel by PCR
Overview
October, 2009
PathGroup Labs is pleased to announce the
immediate availability the FDA-cleared
Luminex Respiratory Virus Panel by PCR
assay. This assay detects the following 10
different common respiratory viruses from a
single nasopharyngeal specimen: Influenza A
(subtype H1), Influenza A (subtype H3),
Influenza A (other subtype), Influenza B,
Respiratory Syncytial Virus subtype A,
Respiratory Syncytial Virus subtype B,
Parainfluenza 1, Parainfluenza 2, and
Parainfluenza
3
virus,
Human
Metapneumovirus,
Rhinovirus,
and
Adenovirus. This assay will be performed 5
days a week, Tuesday through Saturday, so that
a 24-48 hour turn-around time is achieved. This
quick detection and identification of specific
viral nucleic acids from individuals presenting
with signs and symptoms of respiratory
infection will aid in the diagnosis and treatment
of respiratory viral infection.
Influenza A and Novel H1N1 (swine) Influenza
A: This panel will detect all known Influenza A
viruses and subtype the common seasonal H1
and H3 viruses. It is important to note that this
assay does not directly subtype the novel H1N1
(swine) Influenza A virus that has been in
circulation since Spring, 2009. A specimen that
is positive for the novel H1N1 (swine) Influenza
A will have a result of Influenza A (other
subtype) Detected. Current recommendations
from the CDC and most state health
departments are that specific identification of
the novel H1N1 (swine) Influenza A is not
necessary to begin treatment; the identification
of Influenza A is necessary and sufficient for
treatment.
Clinical Utility
 Detection and identification of common respiratory viruses
Methodology: Reverse-transcription Polymerase Chain Reaction and xMAP Detection
Test Codes: RVP
CPT Codes: 87798 (10 units)
Specimen Collection: Collect a nasopharyngeal specimen using the supplied flocked swabs
and M4 media. Place the swab in the M4 container and break the swab shaft at the scored
mark. Leave the swab head in the media. Do not use wooden shaft swabs or cotton tip swabs.
Dacron head nylon shaft swabs are acceptable but not preferred. Store specimens frozen.
Informative videos on collecting nasopharyngeal specimens can be found at
http://copanusa.com/videos/education/index.php
Shipping and Handling: Transport specimens frozen.
Associated Pathologists, PLC and PathGroup Labs
658 Grassmere Park  Suite 101  Nashville TN  37211  615-562-9300  888-474-5227
Excellence in Pathology and Laboratory Services
Reference Ranges: The reference range for each virus is Not Detected.
Turnaround Time: 1-2 days
References
1.
Anzueto, A. and M. S. Niederman (2003).
"Diagnosis and treatment of rhinovirus
respiratory infections." Chest 123(5): 1664-72.
2. Arruda, E., A. Pitkaranta, et al. (1997).
"Frequency and natural history of rhinovirus
infections in adults during autumn." J Clin
Microbiol 35(11): 2864-8.
3. Brandt, C. D., H. W. Kim, et al. (1969).
"Infections in 18,000 infants and children in a
controlled study of respiratory tract disease. I.
Adenovirus pathogenicity in relation to serologic
type and illness syndrome." Am J Epidemiol
90(6): 484-500.
4. Chidgey, S. M. and K. J. Broadley (2005).
"Respiratory syncytial virus infections:
characteristics and treatment." J Pharm
Pharmacol 57(11): 1371-81.
5. Edwards, K. M., J. Thompson, et al. (1985).
"Adenovirus infections in young children."
Pediatrics 76(3): 420-4.
6. Hayden, F. G. and M. G. Ison (2005). Respiratory
Viruses. ACP Medicine. 2006.
7. Heirholzer J.C. (1992). “Adenoviruses in the
immunocompromised host.” Clin Microbiol Rev.
5(3): 262-74.
8. Kuiken, T., R. A. Fouchier, et al. (2003). "Newly
discovered coronavirus as the primary cause of
severe acute respiratory syndrome." Lancet
362(9380): 263-70.
9. Makela, M. J., T. Puhakka, et al. (1998). "Viruses
and bacteria in the etiology of the common cold."
J Clin Microbiol 36(2): 539-42.
10. MMWR (2007). “Acute respiratory disease
associated with adenovirus serotype 14 – Four
States, 2006-2007.” 56(45); 1181-1184.
11. MMWR (2007). “Update – Influenza Activity –
United States and Worldwide, 2006-07 flu
season, and composition of the 2007-08 Influenza
vaccine”. 56(31):789-794.
12. MMWR (2006). “Brief report – Respiratory
Syncytial virus activity – United States 2005-06”.
55(47):1277-1279.
13. Monto, A. S. (1994). "Studies of the community
and family: acute respiratory illness and
infection." Epidemiol Rev 16(2): 351-73.
14. Mullins, J. A., D. D. Erdman, et al. (2004).
"Human metapneumovirus infection among
children hospitalized with acute respiratory
illness." Emerg Infect Dis 10(4): 700-5.
15. Pitkaranta, A. and F. G. Hayden (1998).
"Rhinoviruses: important respiratory pathogens."
Ann Med 30(6): 529-37.
16. Rota, P. A., M. S. Oberste, et al. (2003).
"Characterization of a novel coronavirus
associated with severe acute respiratory
syndrome." Science 300(5624): 1394-9.
17. Rotbart, H. A. and F. G. Hayden (2000).
"Picornavirus infections: a primer for the
practitioner." Arch Fam Med 9(9): 913-20.
18. Ryan M.a., Gray G.C., et al. (2002). “Large
epidemic of respiratory illness due to adenovirus
types 7 and 3 in healthy young adults.” Clin
Infect Dis. 34(5): 577-82.
19. Stalkup, J. R. and S. Chilukuri (2002).
"Enterovirus infections: a review of clinical
presentation, diagnosis, and treatment." Dermatol
Clin 20(2): 217-23.
20. Turner, R. B. (1998). "The common cold."
Pediatr Ann 27(12): 790-5.
21. van den Hoogen, B. G., J. C. de Jong, et al.
(2001). "A newly discovered human
pneumovirus isolated from young children with
respiratory tract disease." Nat Med 7(6): 719-24.
22. van der Hoek, L., K. Pyrc, et al. (2004).
"Identification of a new human coronavirus." Nat
Med 10(4): 368-73.
23. Williams, J. V., P. A. Harris, et al. (2004).
"Human metapneumovirus and lower respiratory
tract disease in otherwise healthy infants and
children." N Engl J Med 350(5): 443-50.
24. Yarush, L. I. and R. W. Steele (2000). "Diagnosis
and prospective treatment of enteroviral
infections in children." Clin Pediatr (Phila) 39(4):
209-11.
Associated Pathologists, PLC and PathGroup Labs
658 Grassmere Park  Suite 101  Nashville TN  37211  615-562-9300  888-474-5227