Download Proposal-Guidelines

*Times New Roman 12 pt, 1 inch margin, Max Length: 10 pages, 1.15 spacing*
Project Title
Group member names, alphabetic order based on last name.
*Arrange the sections in this order and here are some questions to help you with each section
(but you are not just limited to answering those questions*
Introduction
1.
2.
Important background information (Keep it brief, select the most important to talk about)
What motivates you to do this project (e.g. problem with currently existing solution, lack of
investigation into this specific area, great application, your personal experiences or
identifications, etc.)?
3.
What is your goal for the summer research?
Method of Approach
1. What organism(s) are you working with? (Include the strains used and reasoning for choosing
this strain)
2. What gene(s) are you working on? What does the gene do?
3. How are you manipulating the gene (mutagenesis, insertions, deletions, recombination,
etc.)? What does your plasmid intend to do?
4. How will you test your hypothesis (you manipulate the gene, then what? How do you test the
functions?)
5. How do you assay for your plasmid? Create a preliminary outline of the experiments (esp.
assays) required to test your hypothesis.
Please refer to http://2015.igem.org/Team:Toronto/Experiments as an example.
Software Applications (Optional but preferred)
The software team will have the resources to build a full stack web application to suit your
needs. Is there any component of this project which can benefit from a software tool? Can a
modelling software be developed in order to generate hypotheses related to the wet lab project?
Can a results analysis software be applied to the wet lab project pipeline? Iterative and ongoing
interaction between wet lab and dry lab within the same overall project is the ultimate goal here.
Please refer to http://2015.igem.org/Team:Toronto/Description as an example.
Feasibility
1. Safety: Is the organism safe to work with (must be biosafety level 1)? Easy to work with? Easy
to control? Include the biosafety level of the gene source (organism from which gene is
extracted) and the host (organism into which gene will be introduced). The laboratory team
cannot test with mammals but can work with mammalian cells. Please refer to
http://2015.igem.org/Team:Toronto/Safety as an example.
2. Resources: Does the project use specific techniques that are very hard to carry out or specific
devices that are very hard to access? Do your experiments (esp. functional assays) involve any
highly toxic or volatile chemicals?
3. Time: Is this project do-able in four months? If possible, create a rough timeline of how much
time you anticipate each experiment in the above outline to be carried out. Please refer to
http://2015.igem.org/Team:Toronto/Notebook as an example.
4. Financial Resources: is there a particular part of the project that you anticipate will cost a large
amount of money?
5. What do you expect to achieve by the end of the summer research period?
6. Areas of Intersection: Please demonstrate how this project could be applied to computational
creations for our dry lab. In addition, how can this project be used to show effects on human
populations, with either broad or focused scope? Consider the effects on different group sizes
(individual, community, culture) eg, Does the project focus on a particular group of people or a
specific area? Can it have far-reaching effects on a national or international level? What cultural
changes might accompany the application of the project? Please refer to
http://2015.igem.org/Team:Toronto/Practices as an example.
Application
1. Why and how is the project useful? Is it a novel idea? Why is it better than some already existing
solutions (if there is any)? Feel free to discuss social or economic aspects here.
2. Specifically, how do you intend on using your plasmid or its products (i.e. proteins or RNA)?
What private or public organizations (industries, government, civic organizations) might be
interested in this project? Please refer to http://2015.igem.org/Team:Toronto/Design as an
example.
3. How can the project aid human societies if it were to be implemented? In what ways can you
collaborate with human societies that your project is going to affect? Please refer to
http://2015.igem.org/Team:Toronto/Practices as an example.
Reference
1. Please include a reference list. This will be how we will see if your project is credible within
current scientific knowledge, however we will also take into account the novel nature of some
projects without a wide array of previous research to cite.
2. Do not forget to indicate them in the text WITH appropriate page numbers
3. Please include the exact link for website sources.
4. Use any of the following referencing formats: IEEE or APA.