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Qualifying Questionnaire for Liquid Handling Workstation Updated March 11, 2013 Qualifying Questions A B C D E F Question What application is the LH meant for? 1. Nucleic Acid Isolation (Magnetic bead based or Vacuum based or centrifugation based) 2. Recombinant protein isolation / purification 3. PCR or RT-PCR set up (name the thermocycler you have) 4. Sequencing set up 5. Nucleic acid digestion 6. Oligo-based gene synthesis 7. Other similar protocols eg adaptor ligation, reverse transcription, end polishing etc 8. Next Generation Library Prep 9. ELISA 10. SPE (Solid Phase Extraction) a. Positive pressure b. Negative pressure 11. LLE (Liquid:Liquid Extraction) 12. Microarraying/spotting a. Contact or contactless 13. Peptide synthesis 14. Generic functions a. Cherry pick samples b. Normalization c. Dilution (serial/generic) d. Plate reformatting e. Import/export plate map f. Data security/users protocols password protected 15. Any other application: Sample types you plan on working with (i.e. blood, tissue, cells, human, plant, animal, etc..). What is the expected pipeting range or volume you will be working with (ex: 10 uL to 250 uL, 50 uL to 1 mL, etc...) Do you have time limitations on how quickly you need to prep your samples on the liquid handler (ex: 2 hours of liquid handling time to complete sample processing) Will you require an enclosure around your LH (ex: for cell culture work or viral work.....HEPA or standard plexi glass or just a safety bar around the moving arm) Can you send us your protocol or any special protocol steps? Document1 Customer Response G H I J K What throughput (How many samples to be processed in 8 hours/day)? What plates/filter plates/tubes/vials and formats are involved in your protocol: 96 or 384-well Tubes or vials etc Dimensions of non-conventional formats or tubes etc Would you like to go over the webinar to know how your protocol will be automated on the VERSA systems? Preferred date Previous experience working with liquid handlers or automated equipment: 1. Company 2. Model. Question What is the approximate timeline to finalize purchase decision? L Has budget been allocated to the purchase or is this a budget/grant proposal? M Purchasing method? (Tender process, 3 Quotes required, Free to purchase?) Try to understand the decision making process. Is the person you are speaking an influencer, decision maker or information researcher? What are the 3 most important criteria for your automated liquid handling needs? N O Customer Response We welcome any questions you may have. North American Sales: Aurora Biomed Inc. 1001 East Pender Street Vancouver BCCanada V6A 1W2 Phone: 1.800.883.2918 • 604-215-8700 Email: [email protected] Website: www.aurorabiomed.com Document1 International Sales: Aurora Instruments Ltd. 1001 East Pender Street Vancouver BCCanada V6A 1W2 Phone: 604-215-8700 • Fax: 604-215-9700 Email: [email protected] Website: www.aurora-instr.com