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Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies, LLC CPAC Fall Sponsors’ Meeting NeSSI Workshop Nov 9, 2006 Lab-on-chip approach Simplicity of operation Reduction of moving parts – no (mechanical) pumps Robustness Inexpensive and facile maintenance Dionex DX 800 Process HPLC Confidential - Crystal Vision Microsystems LLC ©2006 2 Initial Approach: Using All Glass Chips Inlet lines Column Detector electrodes PEEK chip mount Confidential - Crystal Vision Microsystems LLC ©2006 3 From Discrete Component Setup… Confidential - Crystal Vision Microsystems LLC ©2006 4 …To New NeSSI Mountable Design Confidential - Crystal Vision Microsystems LLC ©2006 5 “Split flow” principle for stream sampling Liters per minute microliters per minute nanoliters per minute Confidential - Crystal Vision Microsystems LLC ©2006 6 Fluid Flow Strategy Split (secondary) flow Main (primary) flow Confidential - Crystal Vision Microsystems LLC ©2006 7 Quantitation of flow splitting Secondary Flow (ml/min) Secondary Flow vs. Main Flow 3.00 2.50 2.00 1.50 1.00 0.50 0.00 0 50 100 150 200 250 300 Main Flow (ml)min) Measurements on water flowing passively through a 350 mm x 500 mm x 6 cm microchannel on the block, pressure drop ca. 0.1 psi. Confidential - Crystal Vision Microsystems LLC ©2006 8 Rapid Prototyping Using Quick Photolithographic Method Inkjet-printed photomask on transparency film Finished product – glass / polymer chip Confidential - Crystal Vision Microsystems LLC ©2006 9 Functional Integration of m-fluidic LC Chip for On-line Sample Pretreatment sample in diluent in micromixer column mobile phase in Confidential - Crystal Vision Microsystems LLC ©2006 10 Liquid Chromatography for NeSSI™ Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies, LLC Split flow approach to sampling Liters per minute microliters per minute nanoliters per minute sample in diluent in micromixer m-fluidic LC Chip for On-line Sample Pretreatment Pulsed electrochemical detection (on-chip) column mobile phase in Confidential - Crystal Vision Microsystems LLC ©2006 11 Back-Mounted Valves for Flow Control Confidential - Crystal Vision Microsystems LLC ©2006 12 Front Mounted Microfluidic Chip m-fluidic chip Confidential - Crystal Vision Microsystems LLC ©2006 13 NeSSI microLC body machined in Ultem® Confidential - Crystal Vision Microsystems LLC ©2006 14