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Transcript 
Progress Report on
Miniaturized Liquid
Chromatography for
NeSSI
Scott Gilbert, CPAC Visiting Scholar
Crystal Vision Microsystems LLC
Atofluidic Technologies, LLC
CPAC Fall Sponsors’ Meeting
NeSSI Workshop Nov 9, 2006
Lab-on-chip approach




Simplicity of operation
Reduction of moving
parts – no
(mechanical) pumps
Robustness
Inexpensive and facile
maintenance
Dionex DX 800 Process HPLC
Confidential - Crystal Vision Microsystems LLC ©2006
2
Initial Approach: Using All Glass Chips
Inlet lines
Column
Detector
electrodes
PEEK chip mount
Confidential - Crystal Vision Microsystems LLC ©2006
3
From Discrete Component Setup…
Confidential - Crystal Vision Microsystems LLC ©2006
4
…To New NeSSI Mountable Design
Confidential - Crystal Vision Microsystems LLC ©2006
5
“Split flow” principle for stream
sampling
Liters per minute
microliters per minute
nanoliters per minute
Confidential - Crystal Vision Microsystems LLC ©2006
6
Fluid Flow Strategy
Split (secondary) flow
Main (primary) flow
Confidential - Crystal Vision Microsystems LLC ©2006
7
Quantitation of flow splitting
Secondary Flow (ml/min)
Secondary Flow vs. Main Flow
3.00
2.50
2.00
1.50
1.00
0.50
0.00
0
50
100
150
200
250
300
Main Flow (ml)min)
Measurements on water flowing passively through a 350 mm x 500
mm x 6 cm microchannel on the block, pressure drop ca. 0.1 psi.
Confidential - Crystal Vision Microsystems LLC ©2006
8
Rapid Prototyping Using Quick
Photolithographic Method
Inkjet-printed photomask on
transparency film
Finished product – glass / polymer
chip
Confidential - Crystal Vision Microsystems LLC ©2006
9
Functional Integration of m-fluidic LC
Chip for On-line Sample Pretreatment
sample in
diluent in
micromixer
column
mobile
phase in
Confidential - Crystal Vision Microsystems LLC ©2006
10
Liquid Chromatography for NeSSI™
Scott Gilbert, CPAC Visiting Scholar
Crystal Vision Microsystems LLC
Atofluidic Technologies, LLC
 Split flow approach to sampling
Liters per minute
microliters
per minute
nanoliters
per minute


sample in
diluent in
micromixer
m-fluidic LC Chip for On-line Sample
Pretreatment
Pulsed electrochemical detection
(on-chip)
column
mobile phase in
Confidential - Crystal Vision Microsystems LLC ©2006
11
Back-Mounted Valves for Flow
Control
Confidential - Crystal Vision Microsystems LLC ©2006
12
Front Mounted Microfluidic Chip
m-fluidic chip
Confidential - Crystal Vision Microsystems LLC ©2006
13
NeSSI microLC body machined in
Ultem®
Confidential - Crystal Vision Microsystems LLC ©2006
14
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