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BIOLOGY 204 GENERAL MICROBIOLOGY INSTRUCTOR: Dr A. Hemsley ANTELOPE VALLEY COLLEGE SPRING 2008 THEORY TEST 1 100 POINTS STUDENT NAME: ___________________________________ STUDENT I.D. NUMBER: _____________________________ • • • • • • • Perusal time = 10 minutes Test time = 90 minutes ANSWER ALL QUESTIONS Read ALL questions carefully during perusal time, you may write if you wish. Use the back of each sheet if you do not have enough writing space, but please label each answer. Question subheadings are NOT of equal points value. Points allocated for each subheading are shown at the end of the question. GOOD LUCK! QUESTION 1 (Total =10 points) Match entries in the left column with answers from the lettered choices in the right column. Write your letter choice to the far left of each question in the spaces provided. Use each answer only ONCE. (1 Point per correct pair) _____ 1. Physician who developed the first vaccine a. Lavoisier _____ 2. Microbiologist who discovered antibiotics from soil bacteria b. Prokaryotes _____ 3.Theory proposing that life forms could only arise from pre-existing life forms c. Edward Jenner _____ 4. Domain whose members contain a true nucleus d. Fungi _____ 5. Cells that lack a nucleus e. Bacteria _____ 6. Scientist who proved the Biogenesis theory f. Biogenesis Theory _____ 7. Kingdom whose members have a chitin cell wall g. Dubos _____8. Scholar who believed oxygen was needed for spontaneous generation h. Protists _____ 9. Domain whose members have a peptidoglycan cell wall i. Eukarya _____ 10. Kingdom whose members are all single celled eukaryotes j. Pasteur page 1 QUESTION 2 (Total =10 points) a. Name and describe at least three levels of structure found in proteins b. What type of molecule is shown below? have learned about) (1) (6) (Hint: think of the 4 classes of biological macromolecule you Explain why you think so. (3) page 2 QUESTION 3 (Total =20 points) A. Give a brief description of the following terms: Isotope (6) Biological Polymer Anabolic reaction B. Aspartic acid, an amino acid, has the following chemical formula: COOH CH2 O NH2 C H C OH Atomic weight H = 1 C = 12 O = 16 N = 14 What is the molecular weight of Aspartic acid? Show your working. (4) What is the weight of 3 moles of Aspartic acid? Show your working. (5) Aspartic acid is one of the components of Nutra Sweet . How many molecules are present in 2.5 moles of NutraSweet? (Avogadro’s number = 6.02 x 10 23) (5) Page 3 QUESTION 4 (Total =20 points) Label the diagrams on this page. Provide a function for each of the structures you have labeled. page 4 QUESTION 5 (Total =20 points) Fill in the blanks in the following sentences. (1 point per correct sentence) 1. One structure that bacteria can use for motility is the ____________________________. 2. A sticky polymer attached to outside of the bacterial cell wall is the ____________________________. 3. Appendages that bacteria use for conjugation are known as ____________________________. 4. The cell wall of Gram positive bacteria contains small amounts of the substance known as ____________________________. 5. The variable thickness of the bacterial cell wall gives a differential color staining reaction when the ____________________________ stain is applied . 6. ____________________________ is a waxy lipid found in the wall of bacteria that cause tuberculosis. 7. The bacterial electron transport chain is located in the ____________________________. 8. Some microbes (like the Penicillium mold) are able to produce ______________________ which can kill other microbes. 9. ____________________________ microscopy is the commonest method for viewing stained specimens. 10. ____________________________ microscopy views bacteria such as Treponema illuminated against a dark background. 11. ____________________________ microscopy uses ultraviolet light instead of visible light. 12. ____________________________ microscopy visualizes internal cellular components due to refractive differences. 13. _____________________________________________ uses two light beams and often computer-colorizes resultant images . 14. Two genera that form endospores are ____________________________ and ____________________________. 15. ________________________ lenses focus the X-rays used by the electron microscope 16. Bacteria that use simple inorganic substances and chemical energy to synthesize their organic requirements are called _________________________. 17. The energy producing potential of nutrient molecules is related to the number of ____________atoms that they possess. 18. Sequential oxidation/reduction reactions are known as ___________________________ reactions. 19. The difference between anaerobic and aerobic respiration lies in the type of _______________________________________. 20. In the metabolic process known as _________________________________, the terminal electron acceptor is an organic molecule. page 5 QUESTION 6 (Total =20 points) A. Give a brief description of the process of RESPIRATION. In your answer be sure to include mention of the 2 separate pathways involved, and the 2 different types of respiration used by microbes and their energy yields. (8) B. Describe 2 ways in which FERMENTATION differs from RESPIRATION? (4) C. Describe the role of NADH and FADH2 in energy extraction processes. (4) D. Explain why Lactobacillus grows slowly, even under ideal nutritional conditions. (4) page 6