Download Table S1

Table S1: Cryptococcus gattii proteins differentially expressed during growth with and without FLC
Fold change (compared to 3 h)2
Protein name (putative)
Accession #
Immune/stress response
Cation-transporting ATPase
Hsp70
Q55SS2
Q55UJ0
Heat shock protein 70
Chaperone
Q4P1U5
Q5KQ06
UDP-glucose dehydrogenase
Q55MH3
Signal transduction
14-3-3 protein
Q5K8Z6
Rab11 protein
Q55SK4
RAN small monomeric GTPase
Q5KGN7
Ribosomal proteins
40S ribosomal protein S13
60S ribosomal protein L20
60s ribosomal protein l30-1 (L32)
60s ribosomal protein l38 (Yml38)
Ribosomal protein s5-1
Q5KIJ0
Q55KZ4
Q5KPM8
Q5KJU6
Q5K947
Ribosomal protein S11
Ribosomal protein S17
Ribosomal protein L13
Ribosomal protein L15
Q5KNH2
Q5KIH4
Q5K7W8
Q5KJD4
Function description1
Response to stress; protein folding
Heat shock protein 70 family; involved in
pleiotropic drug resistance via sequential activation
of PDR1 and PDR5
Heat shock protein 70 family; ATP binding
Chaperone; assist in protein folding/unfolding and
assembly/disassembly
Nucleotide-sugar synthesis; essential for growth at
37°C and for capsule biosynthesis
Regulatory protein; signal transduction; play
important roles in a wide range of vital regulatory
processes
Small GTPase mediated signal transduction;
involved in the exocytic pathway; mediate intraGolgi traffic or the budding of post-Golgi vesicles
from the trans-Golgi
Small GTPase mediated signal transduction
Component of ribosome
Component of ribosome; translation
Component of ribosome; translation
Component of ribosome; translation
Component of ribosome; translation; essential for
viability
Component of ribosome; translation
Component of ribosome; translation
Component of ribosome; translation
Component of ribosome; translation
Untreated
FLC-treated
4h
6h
4h
6h
---
I
I
---
---
N
N
N
+2*
-S
-+1.5
+2.4
+2.2
--
I
S
+3*
N
+1.8
--
I
--
--
--
I
--
--
+1.5
-S
S
+3
-2*
I
N
N
-2.5*
S
---+2.3
+2
---+1.6
--S
--
I
I
+1.5
I
-----
-----
Ribosomal protein l34-b
Ribosomal protein L27
Ribosomal protein L36
Ribosomal protein of the small subunit
Small subunit ribosomal protein 14
40S ribosomal protein S4
Large subunit ribosomal protein L3
Ribosomal protein l5-b
Q5KN25
Q55YU7
Q5KEE1
Q5KGW0
Q5KFT0
Q5KNK2
Q5K9E3
Q5KJU2
Ribosomal protein l23
Ribosomal protein of the large subunit
Q5K959
Q5KL89
Sugar/lipid metabolism
6-phosphofructokinase
Q5KAN4
Acetyl-CoA carboxylase
Isocitrate dehydrogenase (NAD+)
Q55QT6
Q5KAD7
Fatty acid synthase alpha subunit
Q2HYU3
Fructose-bisphosphate aldolase
Glucose-6-phosphate isomerase
Glutamine-fructose-6-phosphate
transaminase (Isomerizing)
Glyceraldehyde-3-phosphate
dehydrogenase (Fragment)
NADPH dehydrogenase 2
Q5KMW2
Q55Y45
Q5KII0
Oxoglutarate dehydrogenase
(Succinyl-transferring)
Q5KMH0
Transketolase
Q55T38
Fatty acid synthase complex protein
Phosphoglucomutase
Q5KG98
Q5K7B5
Pyruvate kinase
Q5KKG5
Nuclear proteins
B3TGN5
Q55N61
Component of ribosome
Component of ribosome
Component of ribosome; translation
Component of ribosome; translation; rRNA binding
Component of ribosome; translation
Component of ribosome; translation; RNA binding
Component of ribosome; translation
Component of ribosome; translation; 5S rRNA
binding
Component of ribosome; translation
Component of ribosome; translation
-2
S
-I
S
+2
N
S
-2*
+3*
I
I
+2
+2
+1.5
+2
I
-----1.8
-I
--I
---2.5*
I
--
S
S
+2
+3
-S
I
-3.5*
Glycolysis; convert D-fructose-6-phosphate into Dfructose 1,6-biphosphate
Fatty acid biosynthetic process
TCA cycle; Isocitrate dehydrogenase (NAD+)
activity
Fatty acid biosynthesis; magnesium ion;
bindingtransferase and synthase activities
Glycolysis; fructose-bisphosphate aldolase activity
Glycolysis; glucose-6-phosphate isomerase activity
Carbohydrate biosynthetic process
--
I
--
I
-2.2
--
N
I
---
---
N
+3
--
--
S
---
+3*
I
--
----
I
-I
N
-1.8
--
--
--
--
--
I
--
I
--
I
--
I
--
--
N
--
+2
--
---
I
I
N
+2
--
I
Glycolysis; glyceraldehyde-3-phosphate
dehydrogenase (phosphorylating) activity
Oxidoreductase activity; may be involved in sterol
metabolism
Glycolysis; oxoglutarate dehydrogenase (succinyltransferring) activity; energy production in TCA
cycle
Catalytic activity in the pentose phosphate pathway;
needed for synthesis of aromatic amino acids
Fatty acid biosynthesis
Carbohydrate metabolic process; hexose
metabolism
Glycolysis; carbohydrate degradation
Curved DNA-binding protein homolog
Histone H4
Q5KJ40
Q5K8H5
Small nuclear ribonucleoprotein E
Q5KBD5
Protein/amino acid metabolism
Aminoadipate-semialdehyde
dehydrogenase
Aspartate carbamoyltransferase
DNA binding; hydrolase activity
Core component of nucleosome; play a central role
in transcription regulation, DNA repair, DNA
replication and chromosomal stability
Nucleic acid binding
S
N
N
+2
-S
I
-2*
--
--
S
S
Lysine biosynthesis; cofactor/phosphopantetheine
bindings
Pyrimidine base biosynthesis, glutamine
metabolism
Protein folding; unfolded protein binding; ATP
binding
Protein biosynthesis; translation; together with
other initiation factors to stimulate binding of
mRNA and methionyl-tRNAi to the 40S ribosome
Protein biosynthesis; translation; together with
other initiation factors to stimulate binding of
mRNA and methionyl-tRNAi to the 40S ribosome
Translation initiation factor activity
--
I
--
--
S
+2.5*
S
+3.6*
--
I
--
--
S
N
--
--
S
-2*
--
--
--
I
--
I
--
I
S
S
S
+1.5
--
--
S
N
--
--
---
I
I
---
I
--
S
S
S
+3
---
I
--
--
I
--
I
Q5K8B4
ATP-dependent peptidase activity; serine-type
endopeptidase activity
Protein transport; regulation of transcription
S
N
--
--
Q5KCH5
Protein transport; regulation of transcription
S
+2.5
--
--
Q5KEK6
Q5KNM2
ER-associated protein catabolismrelated protein
Eukaryotic translation initiation factor
3 subunit A
Q5KHB5
Eukaryotic translation initiation factor
3 subunit C
Q5KH72
Eukaryotic translation initiation factor
5C homolog
FK506-binding protein 1 (Peptidylprolyl cis-trans isomerase)
FK506-binding protein 4 (Peptidylprolyl cis-trans isomerase)
GrpE protein
Q5KI79
Q55K54
Homocitrate synthase
Homoisocitrate dehydrogenase
Q5KIZ5
Q55U60
Isoleucine-tRNA ligase
Mitochondrial processing peptidase
beta subunit, mitochondrial (Betampp)
MMS2
Q5KPM2
Q5KED7
Nascent polypeptide-associated
complex subunit alpha
Nascent polypeptide-associated
Q5KGK5
O94746
Q5KIJ5
Q5KA71
Protein folding; catalyse the cis-trans isomerization
of proline imidic peptide bonds in oligopeptides
Protein folding; catalyze the cis-trans isomerization
of proline imidic peptide bonds in oligopeptides
Protein folding and transport into mitochondrial
matrix
Lysine biosynthesis; transfer acyl groups
Lysine biosynthesis; act on the CH-OH group of
donors, NAD or NADP as acceptor
Isoleucyl-tRNA aminoacylation; ATP binding
Proteolysis; metalloendopeptidase activity; zinc ion
binding
complex subunit beta
Proline-tRNA ligase
Protein TIF31 homolog
Serine hydroxymethyltransferase
T-complex
Q55YK1
Q5K7G8
Q5KAU8
Q55K28
T-complex protein 1, theta subunit
(Tcp-1-theta)
Translation initiation factor
Adenosylhomocysteinase
Arginine-6 protein
Polyubiquitin
Q5KKB4
Q5KMN3
Q5KJ87
Q55QU3
O35079
Plasma membrane proteins
ATP-binding cassette transporter
A3QWE5
Cassette (ABC) transporter
Isoprenoid biosynthesis-related protein
Q5KJ82
Q5KG83
Cytoskeleton proteins
Actin lateral binding protein
Alpha tubulin
Q5KF76
Q5KM62
Beta1-tubulin
Q5KKE7
Actin
Q5KP06
Miscellaneous
Aldehyde reductase
Q55K68
C1-tetrahydrofolate synthase
Q55NZ3
Chaperone regulator
Q5KLR7
Clathrin heavy chain 1
Q5KA29
Prolyl-tRNA aminoacylation
Translation initiation factor
One-carbon metabolism; serine metabolic process
Protein folding; unfolded protein binding; ATP
binding; required for the assembly of actin and
tubulins
Protein folding in cytoplasm; chaperone; unfolded
protein binding; ATP binding
Protein synthesis; GTP binding
Homocysteine biosynthesis; 1 carbon metabolism
Arginine biosynthesis, mitochondrion
Protein modification process
S
S
S
S
+2
+1.5
-3
S
-----
-----
--
I
--
--
-S
-+1.4
I
N
-+1.6
---+1.5
-I
I
-4*
Protein transport and binding; integral into
membrane; coupled to transmembrane movement
of substances
Protein transport and binding
Isoprene biosynthesis to form lanosterol
--
I
I
--
---
I
I
---
-I
Bind to and stabilize actin cables and filaments
Microtubule-based movement; protein
polymerization; structural molecular activity
Microtubule-based movement; protein
polymerization
Cytoskeleton; involved in various types of cell
motility
---
I
I
---
-I
S
N
--
--
S
+1.5
--
I
Oxidoreductase activity; alcohol dehydrogenase
(NADP+) activity
Folic acid and derivative biosynthesis; involved in
single carbon metabolism; required for biosynthesis
of purines, thymidylate, methionine, and histidine
Regulation of the HSP90 and HSP70 functions;
protein translocation across membranes
Intracellular protein transport; vesicle-mediated
transport; clathrin coat
--
I
--
I
S
N
S
N
S
+2
--
--
--
I
--
--
Cytochrome c oxidase subunit 2
Q85SZ4
Cytochrome c oxidase subunit V
Dihydrolipoyl dehydrogenase
Q5K946
Q5Y229
Hydrogen-transporting ATP synthase
Inorganic diphosphatase
Mitochondrial C1-tetrahydrofolate
synthase
Mitochondrial import inner membrane
translocase subunit TIM8
Q5KJV2
Q5KHF9
Q55NZ3
Mitochondrial import inner membrane
translocase subunit TIM10
Q55U43
NADH dehydrogenase 10.5K chain
NADH-ubiquinone oxidoreductase
30.4 kDa subunit
Nucleoside-diphosphate kinase
Pre-mRNA splicing factor
RNA-binding G protein effector
homolog
Q5KCH4
Q5KF23
Q5KFM0
Q55KK1
Q55XU9
Q55N75
Transferase
Q5KHG0
U6 snRNA-associated Sm-like protein
LSm2 homolog
Ubiquinol-cytochrome C reductase
iron-sulfur subunit, mitochondrial
ATP synthase complex subunit H
Complex 1 protein
Q55VD7
Electron transporter, transferring
electrons within CoQH2-cytochrome c
reductase complex
NADH dehydrogenase
Q5KFT4
Q5KGU5
Q5KIZ7
Q5KNR5
Q5KN57
Respiratory electron transport chain; form the
functional core of the enzyme complex; electron
transfer
Electron transfer in respiratory chain
Cell redox homeostasis; dihydrolipoyl
dehydrogenase activity
ATP hydrolysis coupled proton transport
Phosphate metabolic process
Folic acid and derivative biosynthesis; ATP binding
--
I
N
+1.5
S
I
N
I
---
---
-S
S
I
+1.6
N
S
---
S
---
S
+2
--
--
--
I
S
S
-S
I
N
---
---
S
---
N
I
I
----
----
--
I
--
--
--
I
--
--
Electron transport; respiratory chain in
mitochondrion
ATP synthesis; hydrogen ion transport
Transfer of electrons from NADH to the respiratory
chain
Respiratory chain; electron carrier activity;
heme/iron ion bindings
--
I
--
--
S
N
S
S
S
N
S
S
S
N
I
--
Oxidoreductase activity; FAD binding
--
I
--
I
Intracellular protein transmembrane transport;
protects the hydrophobic precursors from
aggregation and guide them through the
mitochondrial intermembrane space
Intracellular protein transmembrane transport;
protects the hydrophobic precursors from
aggregation and guide them through the
mitochondrial intermembrane space
Respiratory chain complex 1
Electron transfer in respiratory chain
Nucleoside diphosphate kinase activity
mRNA processing
Mating response pathway; mainly associated with
nuclear envelope and ER; interact in mRNAdependent manner with translating ribosomes
Transferase activity; required for xanthine
utilization and for optimal utilization of guanine
mRNA processing
NADH-ubiquinone oxidoreductase
Polyadenylate-binding protein,
cytoplasmic and nuclear
Q55XC1
Q5KBW2
Rab/GTPase
Q5KK41
Succinate dehydrogenase flavoprotein
subunit
Ubiquinol-cytochrome C reductase
complex core protein 2
UTP-glucose-1-phosphate
uridylyltransferase
Q5KBA0
Unknown
Cytoplasm protein
Cytoplasm protein
Predicted protein, ATP-dependent
Uncharacterized protein
Uncharacterized proteins
Uncharacterized protein
ATP synthesis coupled electron transport
mRNA processing/transport; regulation of
translation; an important mediator of the multiple
roles of the poly(A) tail in mRNA biogenesis,
stability and translation
Protein transport; essential for exocytosis; may
regulate polarized delivery of transport vesicles to
the exocyst at the plasma membrane
Electron transport chain; FAD binding
-N
-N
---
I
I
--
I
S
S
--
--
--
I
S
N
--
I
Q5KKA5
Electron transport; metalloendopeptidase activity;
zinc ion binding
Nucleotidyltransferase activity; catalyse the
reversible formation of UDP-Glc from glucose 1phosphate and UTP
S
N
--
I
Q5KAL4
Q55ZC0
A0CAI2
Q5KJK9
Q55SN6
Q55PA2
Unknown
Unknown
Unknown
Unknown
Unknown
Unknown
S
------
N
------
--I
S
S
S
-I
-S
S
S
Q5K8U4
1
Searches based on genome data for Cryptococcus species, or for other fungi if not available.
2
Based on ratio of normalised spectrum counts. I: induced (only present at that time point and not at other time points); S: suppressed (not
present at that time point but found at other time points); N: no change; -- : protein absent at that time point. * indicates significant difference (p
< 0.05).