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SUPPLEMENTARY INFORMATION
Targeting human embryonic stem cells with quantum dots conjugated phages
Wenxiu Zhao1, Lei Jin1, Hang Yuan1, Zhiyang Tan1, 3, Changhua Zhou2, *Lin Song Li2
and *Lan Ma1
Division of Life Science & Health, Graduate School at Shenzhen, Tsinghua University,
Shenzhen, China.
1
Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004,
P. R. China.
2
3
School of Life Sciences, Tsinghua University, Beijing, China.
*Corresponding author
Correspondence
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Supplemental Figure S1. ESCs culture and identification. (A) Human embryonic cell
(hESC) culture and AKP stains: (a) human ESCs, (b) differentiated human ESCs, (c)
AKP stain for hESCs, and (d) AKP stain for differentiated hESCs. (B)
Immunocytochemical staining For ESC markers. (a,b)Nanog; (c,d)Oct4; (e,f)SSEA-4;
(g,h)TRA-1-60. (C) Monkey ESCs rs366.4 (a) mouse ESC line R1 (b), and mouse ESC
line E14 (c); (d-f): AKP stains of Monkey ESCs rs366.4, mouse ESC line R1 and
mouse ESC line E14. Scale bar, 50 μm. (D) RT-PCR analyses for ES cell marker
genes. All ESC were cultured on mouse embryo fibroblast (MEF) feeder cells.
Supplemental Figure S2. Fluorescence signal channels of immunocytochemistry of
phage clone with cells. (A) Green channel shows fluorescence signals of phages; (B)
Blue channel shows fluorescence signals of nucleus.
Supplemental Table S1. RT-PCR primers for ESCs marker genes detection.
Gene
Forward primer (5’- 3’)
Reverse primer (5’ – 3’)
Klf4 (mouse)
TGCCTTGCTGATTGTCTATT
GCAGTGTCTTCTCCCTTCC
Oct4 (mouse)
AAAGCGAACTAGCATTGAGA
AGCAGTGACGGGAACAGA
Nanog (mouse)
GATTCTTCTACCAGTCCCAAAC
ATGCGTTCACCAGATAGCC
Sox2 (mouse)
TCTTCCTCCCACTCCAGG
TAGTCGGCATCACGGTTT
Sox2 (macaca)
CCCTGTGGTTACCTCTTCC
CTCCCATTTCCCTCGTTT
Nanog (macaca)
TTATTGTGGCGGTGACTC
TTGCCTTTGGGACTGGT
Oct-4 (macaca)
GACAACAATGAGAACCTTCA
CACATCCTTCTCTAGCCCAA
Sox2 (human)
CGCCCCCAGCAGACTTCACA
CTCCTCTTTTGCACCCCTCCCATTT
Nanog (human)
TGAACCTCAGCTACAAACAGGTA
AACTGCATGCAGGACTGCAGAG
Oct-4 (human)
AGAAGGATGGTCCGAGTGTG
CCACCCTTTGTGTTCCCAATTCC
Supplemental Table S2. Homological sequences of H166 peptide.
Genbank
Name
accession
BAC86301.1
Unnamed protein
product [Homo
sapiens]
AAN78464.1
T cell receptor beta
chain CDR3 region
[Homo sapiens]
CAB56534.1/22791
Fibrillin [Homo
6
sapiens]
EAW94640.1
Sterile alpha motif
domain containing
14, isoform CRA_a
Homolgy sequences
AAO85044.1
G protein-coupled
receptor PGR6
AAH05830.2
Annexin A9 [Homo
sapiens]
BAC85535.1
Unnamed protein
product [Homo
sapiens]
CAC33425.1
Unnamed protein
product [Homo
sapiens]
EAW88662.1
CD163 antigen,
isoform CRA_a
[Homo sapiens]
NP_001003682.1
Tran membrane
protein 200B
[Homo sapiens]
NP_004235.4
Scavenger receptor
cysteine-rich type 1
protein M130
isoform
a precursor [Homo
sapiens]
XP_003403681.1
Deleted in
malignant brain
tumors 1 protein
isoform
BAD18710.1
Scavenger receptor
protein family
member
EAW90667.1
hCG2040041
[Homo sapiens]
EAW52262.1
hCG1791667
[Homo sapiens]
CAE91957.1
Unnamed protein
product [Homo
sapiens]
EAW89811.1
WDR45-like,
isoform CRA_d
[Homo sapiens]
EAX02399.1
EF-hand calcium
binding domain 4A
[Homo sapiens]
AAR13893.1
MLL5 [Homo
sapiens]
EAX06196.1
Nephronectin,
isoform CRA_c
[Homo sapiens]
CAA80544.1
M130 antigen
extracellular variant
[Homo sapiens]
EAW98118.1
WD repeat and
SOCS box
containing 2 [Homo
sapiens]
A6NCK2.3
Full=Tripartite
motif-containing
protein 43B
CAC33425.1
Unnamed protein
product [Homo
sapiens] Secreted
polypeptides
encoded thereby
Supplemental Table S3. Homological sequences of H178 peptide.
Genbank
Name
accession
EAW89544.1
Lectin, galactosidebinding, soluble, 3
binding protein,
isoform CRA_b
[Homo sapiens]
ACK56072.1
Leukocyte
immunoglobulinlike receptor [Homo
sapiens]
AAF65226.1
E2F transcription
factor 4[Homo
sapiens]
AAH30029.1
Inhibin, beta B
[Homo sapiens]
AAY57157.1
Immunoglobulin
heavy chain
variable region
AAC97255.1
T-cell receptor beta
chain
Homolgy sequences
NP_005558.1
Galectin-3-binding
protein precursor
[Homo sapiens]
NP_001138930.1
Natural cytotoxicity
triggering receptor
1
[Homo sapiens]
NP_001229510.1
ras and Rab
interactor 2 isoform
NP_001240286.1
Tyrosine-protein
kinase receptor Tie1 isoform 2 [Homo
sapiens]
NP_005415.1
Tyrosine kinase
with
immunoglobulinlike and EGF-like
NP_659403.4
FRAS1 related
extracellular matrix
1 [Homo sapiens]
NP_001035148.1
Macrosialin
isoform B precursor
[Homo sapiens]
CD68
NP_722577.2
Probable G-protein
coupled receptor
113 isoform 3
NP_110447.2
Collagen alpha1(XXI) chain
precursor [Homo
sapiens]
NP_001001396.1
Plasma membrane
calciumtransporting
ATPase 4 isoform
4a [Homo
sapiens]
NP_005121.1
Ibroblast growth
factor-binding
protein 1 precursor
[Homo sapiens]
NP_000564.2
Complement
receptor type 1
isoform F precursor
[Homo sapiens]
NP_001002264.1
Epithelial-stromal
interaction protein 1
isoform 1 [Homo
sapiens]
NP_001035194.1
Mucin-17 precursor
[Homo sapiens]
NP_612356.1
Zinc finger protein
551 [Homo sapiens]
NP_005374.2
Sialidase-2 [Homo
sapiens]
NP_001018056.1
SPS1/STE20related protein
kinase YSK4
isoform 2 [Homo
sapiens]
NP_005069.2
Transducin-like
enhancer protein 3
isoform a [Homo
sapiens]
NP_001164164.1
Cancer-associated
gene 1 protein
isoform 2 [Homo
sapiens]
NP_055858.2
TBC1 domain
family member 9B
isoform b [Homo
sapiens]
NP_958849.1
Transcriptional
enhancer factor
TEF-3 isoform 2
[Homo sapiens]
NP_003350.1
UDP-glucose 6dehydrogenase
isoform 1 [Homo
sapiens]
NP_062547.1
Sushi domaincontaining protein 2
precursor [Homo
sapiens]
NP_001129288.1
LIM domain and
actin-binding
protein 1 isoform 4
[Homo sapiens]
NP_001186851.1
Sialate Oacetylesterase
isoform 2 [Homo
sapiens]
NP_005655.1
Probable E3
ubiquitin-protein
ligase makorin-3
[Homo sapiens]
NP_056040.2
Protein prune
homolog 2 [Homo
sapiens]
NP_002770.3
PH and SEC7
domain-containing
protein 1 [Homo
sapiens]
NP_001129288.1
Protein PCOTH
isoform 2 [Homo
sapiens]
NP_066288.2
Deformed
epidermal
autoregulatory
factor 1 homolog
[Homo sapiens]
NP_078920.2
Lysine-specific
demethylase NO66
[Homo sapiens]
NP_001171696.1
BEN domaincontaining protein 2
isoform 2 [Homo
sapiens]
NP_115806.1
Serine/threonineprotein kinase
BRSK1 [Homo
sapiens]
XP_001715329.2
Hypothetical
protein LOC338667
[Homo sapiens]
NP_001001396.1
plasma membrane
calciumtransporting
ATPase 4 isoform
4a [Homo
sapiens]
NP_663780.2
synemin isoform A
[Homo sapiens]
NP_001241872.1
girdin isoform 3
[Homo sapiens]
NP_000419.1
Latent-transforming
growth factor betabinding protein 2
precursor [Homo
sapiens]
NP_001089.1
aconitate hydratase,
mitochondrial
precursor [Homo
sapiens]
NP_006369.3
Semaphorin-4D
isoform 1 precursor
[Homo sapiens]
NP_996803.2
Apical endosomal
glycoprotein
precursor [Homo
sapiens]
NP_060142.3
Transient receptor
potential cation
channel subfamily
M member [Homo
sapiens]
NP_057058.2
Lambda-crystallin
homolog [Homo
sapiens]