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Enzymes and Metabolism Name: ____________________________ Much of the human digestive process takes place in the stomach and small intestine. Glands secrete enzymes that break down protein chains by chemical reactions. Two important digestive enzymes for proteins are pepsin and trypsin. The effect of the pH on the activity of these enzymes can be determined by analyzing the data below. Plot the data from the data tables on the graph below and then analyze the activity of the two enzymes to answer the questions. Use two different colors for your lines and label accordingly. Make sure that your graph has the axes appropriately labeled and has a descriptive title. Table 1: Activity of Digestive Enzymes Pepsin and Trypsin pH 0.9 1.5 3.0 5.0 5.1 ************************** pH 4.0 5.0 6.5 7.5 9.0 Activity of Pepsin-% of maximum 0 65 100 20 0 ************************** Activity of Trypsin-% of maximum 0 75 100 90 0 Graph 1: ____________________________________________________ Analysis Questions 1. At which pH is pepsin the most active? Trypsin? 2. If pepsin and trypsin had to function together, which pH would produce the highest activity? 3. What do you think would happen if you took an antacid prior to eating a hamburger? Think about this in terms of what buffers do. 4. The stomach is a highly acidic environment. Once food leaves the stomach, it enters the small intestine, where it is neutralized by sodium bicarbonate. Where do you think pepsin and trypsin are the most active individually? Explain. 5. What happens to the enzymes after they have catalyzed their respective reactions under normal environmental conditions? 6. What would happen to the enzymes if the pH or even temperature of the environment totally caused the enzyme to “stop” working? Explain in terms of the active site and what is happening to the enzymes “shape”.