Download What is Bio-technology?

What is Bio-technology?
Why is it important to understand
your genetic make up?
How does this information affect
your life? Society?
Advancement of technology for research associated with life
science. Especially Genetic Engineering (changing genes and
recombinant DNA (changing DNA sequences)
http://www.komonews.com/news/consu
mer/17071161.html
Stolen IPOD? Cell Phone?
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We are going to begin EHS CSI training!
Eliminating crime at EHS by
incorporating electrophoresis into our
discipline policy. Would you like to
help?
Tools you need to learn to use…
Micro pipettes…
 Electrophoresis…
 Before we can use these tools we will need
training on how they work…
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What is the metric system?
.
Standard measurement
system
used through out the world
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How big is a Liter?
How big is a mili Liter?
mL(1/1000 L) mili means one thousanth
How big is a micro liter compared to a L and a
mL?
(1/1000 of a mL) (1/1,000,000 of a L)
Which of these units do you think would be the
best for looking at pieces of DNA?
Why?
The MICRO LITER!!
DNA IS SO SMALL THAT IT NEEDS TO
BE LOOKED AT IN VERY TINY PIECES
 What tool should we use to measure DNA?
 Micro-pipettes allow us to take samples as
small as .5 µL and as large as 1000 µL (1
mL)
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Are you a metric system master?
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When I convert from L to mL will the number get
smaller or larger?
Larger, I will move my decimal place 3 spaces to
the right because it is a thousand times smaller.
When I convert from mL to µL will the number
get smaller or larger?
Larger, I will move my decimal place 3 places to
the right.
Metric System Master?
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When I convert from µL to mL will the number
get smaller or larger?
Smaller, I will move my decimal place 3 places to
the left. For example 2 µL = .002 mL
When I convert from mL to L will the number get
smaller or larger?
Smaller. I will move my decimal place 3 spaces to
the left.
2.0 mL = .002 L
Factor Label Method
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A great way to set up conversions
Start the conversion with what you know and
make a relationship that relates to what you want
to know
For example when determining how many
µL are in 20 L set up this conversion
20L
1,000,000 µL
1 L
This means 20 X 1,000,000 =20,000,000 µL
Metric System Master???
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If I have L and want µL what should I do?
Move the decimal place 6 places to the right
Or set a conversion using factor label method
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I will give you a measurement and you must tell me how
many µL I have… Use Factor Label method
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2 µL = how many L?
2 µL 1 L
1,000,000 µL
2µL X 1L /1,000,000µL
=
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.000002L
87 mL = how many µL?
87mL
1,000 µL
1 mL
87mLx 1000 µL/ 1mL
Try these on your own….
100 µL = How many L
 2,300,000 L = How many mL
 45 L = How many µL?
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1. .0001
2. 2,300,000,000mL
3. 45,000,000 µL
EQUIPMENT!!!
You must show competentence before you
can join the EHS crime fighting team!!
 P-10
range of .5-10 µL
 P-20
range of 2- 20 µL
 P-200
range of 20-200 µL
 P 1000
range of 200-1000 µL
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IF YOU USE THE WRONG PIPETTE IT
WILL MAKE THEM LESS ACCURATE
AND MAY DESTROY THEM..AND THEY
MIGHT EXPLODE!
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If I want to measure 2 µL which pipette would I
choose?
P-10
If I want to measure 178 µL which pipette would
I choose?
P-200
If I want to measure 778 µL which pipette would
I choose?
Try these on your own.
Tell me the pipet for the specific
measurement.
 600 µL
25 µL
 225 µL
90 µL
 1 µL
18 µL
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1. P-1000
4. p-200
2. P-1000
5. P-200
3. P-10
6. P-20
Use Factor label method to
determine
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How many mL are in 2,000 чL?
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How many чL are in 3 L?
How to use pipets
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Lets look at our Fabulous handout your
wonderful teacher gave you….
Tommorrow, I’ll be back to…
Make sure you are measuring the correct
amounts of DNA?
 In order to participate in the lab and gain
your CSI Terrace crime fighting skills…
 Finish Metric system/Pipet worksheet
 We’ll practice using pipets tommorrow
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We will continue our CSI EHS crime
fighter training tommorrow…
 Practice your conversions and pipette
choice worksheet tonight!
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