Download Biochemistry_II

Biochemistry II
Unit I Bioenergetics and Biological Oxidation
Short Answers
1. Exergonic and endergonic reactions
2. Second law of thermodynamics – Delta G
3. Redox reactions and reduction potential
4. Structure of Mitochondria
5. Ultrastructure of Chloroplast
6. Uncouplers
7. Substrate level phosphorylation
8. Cyclic Phosphorylation
9. ATP synthase
10. Chemiosmotic theory
Essays
1. High energy compounds
2. Mitochondrial electron transport chain
3. Theories of oxidative phosphorylation
4. Noncyclic and cyclic photophosphorylation
5. Freeradical induced damage to biomolecules
6. Antioxidants
7. Enzymes involced in biological oxidation
Unit II Carbohydrate and lipid metabolism
Short answers
1. Anaplerotic reactions
2. PDH complex
3. C4 pathway
4. Gluconeogenesis
5. Significance of HMP shunt
6. Pasteur effect
7. Synthesis of triglycerides and phospholipids
8. Fatty acid synthase
9. Glycogen synthesis
10. Ketogenesis
Essays
1. Krebs cycle
2. HMP Shunt
3. Glycogen Metabolism
4. Calvin cycle C3 Pathway
5. Beta oxidation of fatty acids
6. Synthesis of fatty acids
7. Biosynthesis of cholesterol
Unit III Nitrogen compounds Metabolism
Short answers
1. Transamination
2. Deamination
3. Glucogenic and ketogenic aminoacids
4. Metabolism of glycine
5. Denovo and Salvage pathways
6. Pyrimidine biosynthesis
7. Phenylketonuria
8. Synthesis of deoxyribonucleotides
9. Thymidylate synthase
10. Gout and Lesch Nyhan syndrome.
Essays
1. General reactions of Aminoacid Metabolism
2. Urea cycle
3. Metabolism of phenylalanine and tyrosine. Inborn errors
associated with it.
4. Purine biosynthesis
5. Catabolism of purines
6. Biosynthesis of phenylalanine and tyrosine
7. Diseases associated with nucleic acid metabolism
8. Biosynthesis and degradation of heme.
Unit IV Biochemical Techniques
Short Answers
1. TLC
2. Ion exchange resins
3. Beer Lamberts Law
4. Half life
5. Agarose gel electrophoresis
6. Homogenisation techniques
7. Principle of gel filtration
8.Differential Centrifugation
9. Spectrofluorimetry
10. Beta emitters
Essays
1. Principle instrumentation and applications of
spectrophotometry
2. Gel filtration
3. SDS PAGE
4. Steps in enzyme purification
5. Applications of Radioisotopes
6. Density gradient centrifugation
7. Affinity Chromatography
8. HPLC