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Biochemistry IV
Unit I Microbiology
1. Nutrient media
2. Differential staining
3. Bacterial growth curve
4. One step growth curve/PFU
5.TMV
6. Endospore formation
7. Prions
8. Spirulina
9. Mycoplasma
10. Classification of microorganisms
Essays
1. Ultra structure of bacteria
2. Isolation and cultivation of bacteria
3. Structure and composition of viruses
4. Industrial applications of A.niger and S.cerevisiae
5. Lifecycle of lambda phage
6. Lifecycle of HIV
Unit II DNA Replication and Transcription
1. Semiconservative model of replication
2. DNA Ligase
3. Helicase, topoisomerase
4.Okazaki fragments
5. RNA Polymerase
6. Telomerase
7. Termination of transcription
8. Inhibitors of replication and transcription
9. Bidirectional replication
10. Klenow fragment
Essays
1. Genome organization
2. DNA Polymerases
3. Prokaryotic replication
4. Prokaryotic transcription
5. Processing of mRNA
Unit III Translation
Short Answers
1. Features of genetic code
2. Wobble hypothesis
3.Aminoacyl tRNA synthetases
4. Structure of tRNA
5. Catabolite Repression
6. Attenuation
7. Post translational modifications
8. Inhibitors of Protein Synthesis
Essays
1. Nirenberg and Khorana’s experiments
2. Prokaryotic translation
3. Lac Operon
4. Eukaryotic translation
5.Tryptophan operon
Unit IV Recombinant DNA Technology
Short Answers
1. Restriction enzymes
2. pBR 322 and pUC 18
3. Phage vectors
4. Ti plasmid
5. Southern Blotting
6. Reporter Genes
7. HRT
8. Genomics
9. BLAST
10. NCBI
Essays
1. Outline and tools of gene cloning
2. DNA Sequencing
3. PCR
4. Construction of cDNA Library
5. Screening methods
6. Applications of gene cloning in medicine
7. Applications in plant biotechnology