Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Curriculum Vitae MARK DOUGLAS VAUGHAN Home: 415 E. 23rd Ave. Vancouver, BC V5V 1X7 [email protected] Lab: Department of Chemistry University of British Columbia Vancouver, BC V6T 1Z1 Telephone: (604) 822-9300 Fax: (604) 822-8869 EDUCATION Ph.D., Chemistry, University of Waterloo, 2003. Thesis Title, “Introduction of Novel Chemical Functionalities into Proteins Through Unnatural Amino Acid Mutagenesis,” Supervisor, Dr. J. F. Honek. B.Sc. (Honours), Biochemistry, Dalhousie University, 1998, Thesis Title, “An Assay for Mandelate Racemase Using High Performance Liquid Chromatography,” Supervisor, Dr. S. L. Bearne EXPERIENCE UNIVERSITY OF BRITISH COLUMBIA December, 2003-present Post-doctoral Research Fellow (Supervisor: Professor S. G. Withers, Dept. of Chemistry) Research in the field of biological chemistry, involving enzyme engineering for industrialscale syntheses, investigation of enzyme mechanism, and synthesis of carbohydrate and lipid molecules UNIVERSITY OF WATERLOO September, 1998-November, 2003 Graduate Student/Research Assistant (Supervisor: Professor J. F. Honek, Dept. of Chemistry) Research in biological chemistry, including the synthesis of fluorinated amino acid analogues for application as biochemical and biophysical probes of protein structure and function, mechanistic enzymology, protein purification and characterization, and 19F NMR spectroscopy Teaching Assistant (Supervisor: M. van de Lande, Dept. of Chemistry) Supervision of advanced undergraduate biochemistry laboratories DALHOUSIE UNIVERSITY September, 1998-April, 1998 Undergraduate Research Assistant (Supervisor: Professor S. L. Bearne, Dept. of Biochemistry) Research focused on the development of a novel activity assay for the enzyme mandelate racemase utilizing chiral high performance liquid chromatography to monitor the progress of the enzymatic reaction RESEARCH CONTRIBUTIONS Refereed Journal Publications: Vaughan, M. D., Sampson, P. B., Daub, E., and Honek, J. F. (2005) Investigation of Bioisosteric Effects on the Interaction of Substrates/Inhibitors with the Methionyl-tRNA Synthetase from Escherichia col. Medicinal Chemistry. 1, 227-237. Crepin, T., Schmitt, E., Mechulam, Y.., Sampson, P. B., Vaughan, M. D., Honek, J. F., and Blanquet, S. (2003) Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase. Journal of Molecular Biology. 332, 59-72. Salopek-Sondi, B., Vaughan, M. D., Skeels, M. C., Honek, J. F., and Luck, L. A. (2003) 19F NMR Studies of the Leucine-Isoleucine-Valine Binding Protein: Evidence That a Closed Conformation Exists in Solution. Journal of Biomolecular Structure and Dynamics. 21, 235246. Vaughan, M. D., Sampson, P. B., and Honek, J. F. (2002) Methionine in and out of proteins: Targets for drug design. Current Medicinal Chemistry. 9, 385-409. Vaughan, M. D., Cleve, P., Robinson, V., Duewel, H. S., and Honek, J. F. (1999) Difluoromethionine as a novel 19F NMR structural probe for internal amino acid packing in proteins. Journal of the American Chemical Society. 121, 8475-8478. Bearne, S. L., St-Maurice, M., and Vaughan, M. D. (1999) An assay for mandelate racemase using high performance liquid chromatography. Analytical Biochemistry. 269, 332-336. Patent: Johnson, K. F., DeFrees, S., Withers, S. G., and Vaughan, M. D. Amino acid mutations converting hydrolytic activity of endoglycoceramidase to a synthetic activity. PCT Int. Appl. 47 pp. Conference Presentations (name of primary presenter underlined): Vaughan, M. D., and Honek, J. F. Incorporation of a Novel Chemically Reactive Analogue of Methionine into Bacteriophage Lytic Transglycosylase. Poster presentation, 42nd IUPAC Congress and 86th Canadian Society for Chemistry (CSC) Conference and Exhibition, August 917, 2003, Ottawa, ON. Vaughan, M. D., Daub, E., and Honek, J. F. Efforts toward expanding the substrate selectivity of E. coli methionyl-tRNA synthetase. Oral presentation, 85th Canadian Society for Chemistry Conference and Exhibition, June 1 – 5, 2002, Vancouver, BC. Vaughan, M. D., Daub, E., and Honek, J. F. Evaluation of Fluorinated Methionine Analogues as Substrates for Escherichia coli Methionyl-tRNA Synthetase: Implications in Unnatural Amino Acid Incorporation into Proteins. Oral presentation, 84th Annual Canadian Society for Chemistry Conference and Exhibition, May 26 – 30, 2001, Montreal, Quebec. Vaughan, M. D., Cleve, P., Robinson, V., Daub, E., and Honek, J. F. The difluoromethyl group as a novel 19F NMR probe of protein structure and function. Poster presentation, American Society for Biochemistry and Molecular Biology Joint Meeting: Biochemistry and Molecular Biology ’99, May 16 – 20, 1999, San Francisco, California. Vaughan, M. D., Cleve, P., Robinson, V., Daub, E., and Honek, J. F. The difluoromethyl group as a novel 19F NMR probe of protein structure and function. Poster presentation, 9th Annual Quebec/Ontario Minisymposium in Bio-organic and Synthetic Organic Chemistry, October 30 – November 1, 1998, St. Catharines, Ontario. Bearne, S. L., and Vaughan, M. D. An assay for mandelate racemase using high performance liquid chromatography. Poster presentation, 81st Annual Canadian Society for Chemistry Conference and Exhibition, May 31 – June 4, 1998, Whistler, British Columbia. AWARDS W. B. Pearson Award (University of Waterloo) in recognition of creative research presented in a doctoral thesis, 2004 Nominated for 2004 CAGS/UMI Distinguished Dissertation Award MacNeil Graduate Scholarship in Natural Products Chemistry, 2003 ($1500) NSERC PGS B Graduate Scholarship, May 2001 – April 2003 ($19,100/an) David Holden Memorial Scholarship, 2002 ($750) Guelph-Waterloo Centre for Graduate Work in Chemistry Ph.D. Seminar Prize, 2002 ($100) University of Waterloo Teaching Assistant Award, 2000-2001 ($100) University of Waterloo Research Accomplishment Award, 2001 ($1000) Ontario Graduate Scholarship, May 2000 – April 2001 ($15,000) Ontario Graduate Scholarship in Science and Technology, Jan. 2000 – April 2000 ($5000) University of Waterloo Research Accomplishment Award, 1999 ($500) American Society for Biochemistry and Molecular Biology Student Travel Award, May 1999 ($400 USD) Dalhousie University Faculty of Science Dean’s Honour Roll, 1999. TECHNICAL SKILLS Enzymology – assay development, kinetic analysis, engineering of novel catalytic activities Protein expression and purification Organic synthesis, focusing on amino acids, nucleosides, carbohydrates, glycolipids, and other bioactive compounds Molecular biology techniques – cloning, site-directed mutagenesis Purification techniques for proteins and small molecules: column chromatography, HPLC, and FPLC (ion-exchange, gel filtration, normal and reversed phase, affinity chromatography) NMR spectroscopy of proteins and small molecules, with specialization in 19F NMR Electrospray and MALDI mass spectrometry of proteins and small molecules Molecular Modeling: semi-empirical and ab initio methods using Gaussian 98, PC Spartan, and Jaguar programs Experience in handling radioisotopes, including 32P and 35S Isothermal titration calorimetry, differential scanning calorimetry REFERENCES Dr. Stephen G. Withers Professor Department of Chemistry University of British Columbia Vancouver, British Columbia V6T 1Z1 (604) 822-3402 [email protected] Dr. John F. Honek Professor Department of Chemistry University of Waterloo Waterloo, Ontario N2L 3G1 (519) 888-5817 [email protected] Dr. Stephen L. Bearne Associate Professor Department of Biochemistry Dalhousie University Halifax, Nova Scotia B3H 3J5 (902) 494-1974 [email protected]