Download Experimental Tissue Resource ETR

Experimental Tissue Resource
Facility Description
October 2016
The UC Irvine Experimental Tissue Resource (ETR) provides four major types of service (outlined below). The
ETR is a Shared Resource funded in part by the Chao Family NCI-Comprehensive Cancer Center Support Grant
(P30CA062203) from the National Cancer Institute, and has facilities both on the UC Irvine campus as well as
at the UC Irvine Medical Center. ETR services are available to both on- and off-campus investigators.
i)
Tissue Procurement and Interpretive Histopathology Consultation: The ETR coordinates most
human tissue collection efforts at UCI, providing fresh, frozen or archival human tissues for research use
in a manner consistent with IRB approval. The main campus location of the ETR (Medical Sciences, room
D426D) is a specimen drop-off point for researchers. The ETR also provides human and murine
histopathology evaluation and consultation on a case-by case basis. The medical center facility is located
within the histology lab for the hospital.
ii)
Tissue Histology and IHC services: The ETR provides standard histological services, including
embedding, FFPE and frozen block sectioning, staining (including cytochemical stains) preparation of
recut slides or tissue shavings for nucleic acid extraction and IHC staining. In addition, equipment and
services are provided for immunofluorescence or FISH, automated immunohistochemical (IHC) staining,
optimization of non-standard IHC protocols, and extraction of nucleic acids from archival FFPE tissues
via the Pathology Department’s division of Diagnostic Molecular Pathology. Equipment includes tissue
processors (Sakura Tissue Tek VIP-5 and VIP6), embedding stations (Tissue Tek TEC-5), microtomes
(Sakura), tissue floatation baths, and other routine histology instruments. Automated H&E staining is
performed on a Leica autostainer, and IHC is performed on a Ventana Benchmark ULTRA stainer.
Nucleic acid extractions are prepared in the division of Diagnostic Molecular Pathology (DMP) on a
Promega Maxwell automated DNA extraction robot.
iii)
Laser Capture Microscopy (LCM): The LCM core is located in room D448 in Med Sci I on the UC Irvine
main campus. The core facility consists of a Leica AS-LMD instrument, paired with a Leica CM-3050
cryostat for specimen preparation.
iv)
Mouse Pathology: The ETR provides mouse pathology services (mouse necropsy, histopathology
consultations, consultation on tumor models and experimental design). The Director of the ETR, Dr. Rob
Edwards has over 20 years of expertise with mouse pathology and is a board-certified medical
pathologist. ETR Assistant Dr. Wang has approximately 30 years of animal tumor model experience and
is also available for consult and assistance.