Download Polyclonal Antibody to GABA B receptor GB1b subunit Purified

11-550-C100
Polyclonal Antibody to GABA B receptor GB1b subunit
Purified Antibody (0.1 mg)
Clone:
Polyclonal
Isotype:
Rabbit None
Specificity:
The polyclonal antibody recognizes N-terminus of mouse gamma-aminobutyric
acid (GABA) B receptor 1b. GB1b apparent MW ~100 kDa.
Regulatory Status:
RUO
Immunogen:
Synthetic peptide (coupled with THG) derived from amino acid sequence 30-47 of
mouse GABA B receptor 1b. Only 1 amino acid is different from immunogen
derived from human GB1b.
Species Reactivity:
Mouse, Rat, Other species Not tested
Application:
Western Blotting
Recommended dilution: 2 µg/ml
Immunocytochemistry
Purity:
> 95% (by SDS-PAGE)
Purification:
Purified from rabbit serum by affinity chromatography
Concentration:
1 mg/ml
Storage Buffer:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability:
Store at 2-8oC. Do not freeze. Do not use after expiration date stamped on vial
label.
Expiration:
See vial label
Lot Number:
See vial label
Background:
GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric
acid (GABA), and has important functions in brain by inhibition of adenylyl cyclase
and modulation of G-protein-gated Ca2+ and K+ channels. GABA B receptor is
comprised of two subunits, GB1 and GB2 with N-terminal extracellular and
C-terminal intracellular domains. The GB1 subunit plays a critical role in ligand
binding, whereas the GB2 subunit contains the determinants required for G-protein
signaling. Multiple allosteric interactions between the two subunits are required for
correct functioning of the receptor. There are two N-terminal splice variants of GB1
subunit, termed GB1a and GB1b; their expression in the central nervous system
changes during the ontogenesis and differs between various regions of the brain.
For laboratory research only, not for drug, diagnostic or other use.
EXBIO Praha | Nad Safinou II 341 | 252 50 Vestec u Prahy | Czech Republic
Tel: +420 261 090 666 | Fax: +420 261 090 660 | [email protected] | www.exbio.cz
References:
*Fritschy JM, Meskenaite V, Weinmann O, Honer M, Benke D, Mohler H:
GABAB-receptor splice variants GB1a and GB1b in rat brain: developmental
regulation, cellular distribution and extrasynaptic localization. Eur J Neurosci. 1999
Mar;11(3):761-8.
*Galvez T, Duthey B, Kniazeff J, Blahos J, Rovelli G, Bettler B, Prézeau L, Pin JP:
Allosteric interactions between GB1 and GB2 subunits are required for optimal
GABA(B) receptor function. EMBO J. 2001 May 1;20(9):2152-9.
*Ng GY, Bertrand S, Sullivan R, Ethier N, Wang J, Yergey J, Belley M, Trimble L,
Bateman K, Alder L, Smith A, McKernan R, Metters K, O'Neill GP, Lacaille JC,
Hébert TE: Gamma-aminobutyric acid type B receptors with specific heterodimer
composition and postsynaptic actions in hippocampal neurons are targets of
anticonvulsant gabapentin action. Mol Pharmacol. 2001 Jan;59(1):144-52.
*Margeta-Mitrovic M, Jan YN, Jan LY: Ligand-induced signal transduction within
heterodimeric GABA(B) receptor. Proc Natl Acad Sci U S A. 2001 Dec
4;98(25):14643-8.
*Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G
protein coupling of GABA(B) receptors. Proc Natl Acad Sci U S A. 2001 Dec
4;98(25):14649-54.
*Kniazeff J, Galvez T, Labesse G, Pin JP: No ligand binding in the GB2 subunit of
the GABA(B) receptor is required for activation and allosteric interaction between
the subunits. J Neurosci. 2002 Sep 1;22(17):7352-61.
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer
either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held
responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to
EXBIO´s term and conditions which are available at www.exbio.cz.
For laboratory research only, not for drug, diagnostic or other use.
EXBIO Praha | Nad Safinou II 341 | 252 50 Vestec u Prahy | Czech Republic
Tel: +420 261 090 666 | Fax: +420 261 090 660 | [email protected] | www.exbio.cz