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Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells by Shannon L. McKinney-Freeman, Olaia Naveiras, Frank Yates, Sabine Loewer, Marsha Philitas, Matthew Curran, Peter J. Park, and George Q. Daley Blood Volume 114(2):268-278 July 9, 2009 ©2009 by American Society of Hematology E11.5 AGM-derived repopulating cells do not express CD150 or CD48. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology E12.5 Placenta-derived repopulating cells do not express CD150 or CD48. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology CD150 and CD48 expressing cells are not detectable in E9 YS. Representative analysis of E9 YSderived cells analyzed by flow cytometry for the cell surface expression of c-Kit, CD34, CD41, CD150, and CD48. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology EPOCH cells are phenotypically heterogeneous. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology ESC-HSC are CD41highCD34− and heterogeneous with respect to CD45 cell-surface expression. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology ESC-HSC are CD150+ and heterogeneous with respect to CD48 cell-surface expression. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology Cell surface phenotype of embryonic and ESC-derived HSCs. Our data further defines the cellsurface phenotype of both murine ESC-HSCs and in vivo HSCs during ontogeny, especially with regard to the expression of the SLAM family of cell surface molecules. Shannon L. McKinney-Freeman et al. Blood 2009;114:268278 ©2009 by American Society of Hematology